Anti phospho ser563 hsl

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-phospho-Ser563 HSL is a primary antibody that specifically recognizes the phosphorylated form of Hormone-Sensitive Lipase (HSL) at serine 563. HSL is an enzyme that plays a key role in the regulation of lipolysis, the breakdown of stored fat. This antibody can be used in various applications to detect and study the phosphorylation state of HSL at the Ser563 site.

Automatically generated - may contain errors

Lab products found in correlation

Anti phospho hsl ser660, by Cell Signaling Technology (2 mentions) Anti atgl, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Halt protease phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Anti atp5a, by Abcam (1 mentions) Anti uqcrb, by Abcam (1 mentions) Anti cox7b, by Abcam (1 mentions) Anti β actin, by Abcam (1 mentions) Anti pgc 1α, by Proteintech (1 mentions)

Spelling variants of this product

Anti-HSL (21 citations) Phospho-HSL (Ser563) (12 citations) Phospho-HSL (9 citations) Anti-phospho-HSL (4 citations)

2 protocols using anti phospho ser563 hsl

Western Blot Analysis of Lipid Metabolism

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blots were performed as described [35 (link)] with anti-β-actin(#3700), anti-ATGL(#2138), anti-phospho-Ser563 HSL(#4139) and anti-phospho-Ser660 HSL(#4126) from Cell Signaling (Danvers, MA, USA), and anti-GRP78 (ab21685) and anti-DGAT2 (ab237613) from abcam (Shanghai, China). The polyclonal antibody to mouse HSL was prepared as described [37 (link)]. Levels of individual proteins were estimated from Western blots using Image J software (ImageJ 1.52a, Wayne Rasband, National Institutes of Health, Bethesda, MD, USA).

Zhang X., Zhang C.C., Yang H., Soni K.G., Wang S.P., Mitchell G.A, & Wu J.W. (2019). An Epistatic Interaction between Pnpla2 and Lipe Reveals New Pathways of Adipose Tissue Lipolysis. Cells, 8(5), 395.

+ Open protocol

+ Expand

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues and cells were lysed with RIPA buffer containing Halt Protease/Phosphatase inhibitors (Thermo Fisher Scientifics, Pittsburgh, PA). The primary antibodies used in this study were as follows: anti-phospho (Ser563)-HSL (#4139), anti-phospho (Ser660)-HSL (#4126), anti-HSL (#4107), anti-pPKA Substrate (#9621), and anti-phospho (Ser133)-CREB (#9198) (all from Cell Signaling Technology); anti-SLC22A3 antibody (#ab191446), anti-ATP5A (#ab176569), anti-COX7B (#ab140629), anti-UQCRH (#ab134949), anti-UQCRB (#ab190360), and anti-beta Actin (ab8226) (all from Abcam); anti-UCP1 (#GTX112784) (Genetex); and anti-PGC1α (#20658) (Proteintech Group Inc).

Song W., Luo Q., Zhang Y., Zhou L., Liu Y., Ma Z., Guo J., Huang Y., Cheng L., Meng Z., Li Z., Zhang B., Li S., Yee S.W., Fan H., Li P., Giacomini K.M, & Chen L. (2019). Organic cation transporter 3 (Oct3) is a distinct catecholamines clearance route in adipocytes mediating the beiging of white adipose tissue. PLoS Biology, 17(1), e2006571.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!