The extract weighing 100 mg was transferred into a 10 mL volumetric flask and dissolved in methanol. The solution was then adjusted to the desired volume. Subsequently, it was filtered, and the contents of three curcuminoids were analyzed using their respective calibration curves. The combined value of the curcuminoid contents was reported as the total curcuminoid content.
The analysis was performed using the Agilent 1260 Infinity HPLC instrument (Agilent Technologies, USA). For the separation, an ACE Generix column with dimensions of 150 mm in length, 4.6 mm in internal diameter, and 5 μm in particle size was utilized. An isocratic system consisting of a mixture of acetonitrile (55% v/v) and a 1% acetic acid aqueous solution (45% v/v) was employed at a flow rate of 1 mL/min. The column temperature was maintained at 30°C. A 10 μL injection volume was used. Detection of the analysis response was conducted at a wavelength of 425 nm using a photodiode array detector [27 (link)].
The analysis was performed using the Agilent 1260 Infinity HPLC instrument (Agilent Technologies, USA). For the separation, an ACE Generix column with dimensions of 150 mm in length, 4.6 mm in internal diameter, and 5 μm in particle size was utilized. An isocratic system consisting of a mixture of acetonitrile (55% v/v) and a 1% acetic acid aqueous solution (45% v/v) was employed at a flow rate of 1 mL/min. The column temperature was maintained at 30°C. A 10 μL injection volume was used. Detection of the analysis response was conducted at a wavelength of 425 nm using a photodiode array detector [27 (link)].