Phospho p65 s536

Manufactured by Abcam

Sourced in Germany

Phospho-p65 (S536) is a laboratory reagent used for the detection and quantification of phosphorylated p65 subunit of the NF-kappa-B transcription factor. It is designed for use in various research applications, such as Western blotting, ELISA, and immunohistochemistry, to study the activation and regulation of the NF-kappa-B signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

Anti rabbit ab conjugated to horseradish peroxidase, by GE Healthcare (1 mentions) Supersignal enhanced chemiluminescence system, by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Phospho iκbα ser32 36, by Cell Signaling Technology (1 mentions) Recombinant il 1β, by R&D Systems (1 mentions) Gapdh 0411, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Ml120b, by Merck Group (1 mentions) Iκbα c 21, by Santa Cruz Biotechnology (1 mentions) Col2a1 m2139, by Santa Cruz Biotechnology (1 mentions) Aggrecan 6 b 4, by Abcam (1 mentions) Bufalin, by Merck Group (1 mentions) 5 azacytidine, by Merck Group (1 mentions) Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (1 mentions)

2 protocols using phospho p65 s536

Western Blot Analysis of Cell Signaling

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Cells were lysed in Laemmli buffer and the protein extracts were tested by Ab specific for Sig-1R/OPRS1, HIF-1α, ATF6, p65, phospho-p65 (S536) (all from Abcam), and β-actin (Sigma, Schnelldorf, Germany) diluted at 1:500 and 1:000, respectively. Anti-rabbit Ab conjugated to horseradish peroxidase (GE Healthcare, Little Chalfont Buckinghamshire, UK) was used as the secondary Ab at a dilution of 1:5000. The SuperSignal enhanced chemiluminescence system was used for probing target proteins (Thermo Scientific, Rockford, IL). After the membranes had been probed for Sig-1R/OPRS1 or HIF-1α, they were stripped and re-probed for β-actin.

Szabo A., Kovacs A., Riba J., Djurovic S., Rajnavolgyi E, & Frecska E. (2016). The Endogenous Hallucinogen and Trace Amine N,N-Dimethyltryptamine (DMT) Displays Potent Protective Effects against Hypoxia via Sigma-1 Receptor Activation in Human Primary iPSC-Derived Cortical Neurons and Microglia-Like Immune Cells. Frontiers in Neuroscience, 10, 423.

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Protein Expression and Phosphorylation Analysis

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The antibodies and reagents were purchased from the following sources: NCOA3 (F-2) (Santa Cruz, sc5305), GAPDH (0411) (Santa Cruz, sc47724), COL2A1 (M2139) (Santa Cruz, sc52658), Aggrecan (6-B-4) (abcam, ab3778), COL1A1 (3G3) (Santa Cruz, sc293182), p65 (abcam, ab16502), phospho-p65 (S536) (abcam, ab28856), IκBα (C-21) (Santa Cruz, sc371), phospho-IκBα (Ser32/36) (Cell Signaling, #9246), Goat anti-rabbit IgG-HRP (Santa Cruz, sc-2004), Goat anti-mouse IgG-HRP (Santa Cruz, sc-2302). 5-Azacytidine (Sigma, A2385), Bufalin (Sigma, B0261), BrdU (5-Bromo-2'-deoxyuridine) (Millipore, #19-160), ML120B (Sigma, SML1174), recombinant IL-1β (R&D System, NP-000567).

Zhang B., Li Z., Wang W., Guo J., Kang S., Liu S., Li H., Wang D, & Qi X. (2018). NCOA3 Loss Disrupts Molecular Signature of Chondrocytes and Promotes Posttraumatic Osteoarthritis Progression. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 49(6).

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