Mid output reagent kit

Manufactured by Illumina

The Mid-output reagent kit is a laboratory equipment product designed for use with Illumina's sequencing systems. It provides the necessary reagents and consumables to perform mid-throughput DNA or RNA sequencing experiments.

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Lab products found in correlation

Nextera xt indices, by Illumina (2 mentions) Sp reagent kit, by Illumina (2 mentions) Nextera flex, by Illumina (2 mentions) Nextera xt kit, by Illumina (1 mentions) Nextseq 500, by Illumina (1 mentions)

3 protocols using mid output reagent kit

Nextera Flex Library Preparation and Sequencing

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Libraries were prepared using Nextera Flex (Illumina, San Diego, CA) kits with the Nextera XT indices (Illumina). Barcoded sample libraries were pooled to a concentration of 17.6 ng/μl measured with a high-sensitivity DNA assay on a Qubit (Thermo Fisher Scientific, Waltham, MA) platform. A mid-output reagent kit (Illumina) was used to sequence on the MiniSeq, while an SP reagent kit (Illumina) was used on the NovaSeq platform, both in 2 by 150-bp mode.

Cho M.Y., Oliva M., Spreafico A., Chen B., Wei X., Choi Y., Kaul R., Siu L.L., Coburn B, & Schneeberger P.H. (2021). Two-Target Quantitative PCR To Predict Library Composition for Shallow Shotgun Sequencing. mSystems, 6(4), e00552-21.

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Nextera Flex Library Preparation

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Libraries were prepared using Nextera Flex (Illumina, San Diego, CA) kits with the Nextera XT indices (Illumina, San Diego, CA). Barcoded sample libraries were pooled together to a concentration of 17.6 ng/ul which measured with a high-sensitivity DNA assay on a Qubit (Thermo Fisher Scientific, Waltham, MA) platform. A Mid-output reagent kit (Illumina, San Diego, CA) was used to sequence on the Miniseq, while a SP reagent kit (Illumina, San Diego, CA) was used on the Novaseq platform, both in 2x150bp mode.

Cho M.Y., Oliva M., Spreafico A., Chen B., Xu W., Choi Y., Kaul R., Siu L.L., Coburn B., & Schneeberger P.H.H. (2020). Two-target quantitative PCR to predict library composition for shallow shotgun sequencing.

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Genomic Analysis of Bacteriophages C203 and P206

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Genomic DNA sequencing libraries were prepared using the Nextera XT kit (Illumina, Eindhoven, NL). Sequencing was performed using Nextseq Mid-output reagent kit v2 (2×150 bp) on an Illumina NextSeq 500. Assembly was performed with CLC Genomic Workbench 9.0. The genome was annotated using the RAST server (Overbeek et al., 2014) (link).
Homology searches were conducted with the BLAST tools available at the NCBI website, with PSI-BLAST results supplementing the annotation.
The genome sequences of phage C203 and P206 were deposited in GenBank under the accession nos. MG022439 and MG022440, respectively.

Sváb D., Falgenhauer L., Rohde M., Chakraborty T, & Tóth I. (2018). Identification and characterization of new broad host-range rV5-like coliphages C203 and P206 directed against enterobacteria. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, 64.

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