Recombinant adm

For papain-induced type 2 inflammation in the lung, mice were anesthetized with isoflurane, and then 25 μg of papain or PBS in a total volume of 40 μl was given i.n. on days 1, 2, and 3. BAL fluid and lungs were harvested and analyzed on day 4. For animal model of recombinant ADM treatment, mice were anesthetized with isoflurane, and 25 μg of recombinant ADM (Anaspec), or 100 μg of ADM_22–52 (Anaspec) in a total volume of 40 μl was administered i.n. at indicated time points. Inflammatory cells in the lung were assessed on day 4. For helminth infection, third-stage larvae (L3) of N.b., which was obtained from Dr. D. Artis (Weill Cornell Medicine, New York, NY), were purified with a Baermann technique (Camberis et al., 2003 (link)). After washing three times in PBS, live worms were counted. On day 0, 300 or 500 purified worms were injected subcutaneously in 250 μl PBS. Mice were euthanized at the indicated time points to collect tissues for analysis or to count worm burden. For induction of bleomycin-induced pulmonary inflammation, mice were anesthetized with isoflurane and treated with 0.1 U bleomycin (Sigma-Aldrich) or PBS in a volume of 40 μl intratracheally, followed by analysis on day 7 to assess pulmonary inflammation.