One-cell stage zebrafish embryos were injected with 10 pg of a cDNA construct containing a fluorescent protein attached to a PIP-binding protein domain [Bodipy-PI (Echelon Biosciences Inc)]; PLC-δ-PH-GFP (Tobias Meyer Lab, Stanford University, CA) using a Picopump (World Precision Instruments). At 1 dpf, injected zebrafish were incubated in 0.2 mM phenylthiourea to prevent pigment formation. To image, zebrafish were screened for fluorescent myofibers on a macroscope (Zeiss Axio Zoom) and mounted in 1.5% low-melt agarose on a 3 cm glass-bottom petri dish. All confocal images were taken with a Nikon Eclipse Ti confocal microscope using a 40x oil-immersion lens.
Bodipy pi
Manufactured by Echelon Biosciences
Bodipy-PI is a fluorescent phosphoinositide (PI) analog developed by Echelon Biosciences. It is a derivative of the Bodipy dye family and is designed to serve as a probe for the detection and analysis of PIs in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bodipy pi
1
In Vivo Visualization of PIP Dynamics
2
Visualizing Zebrafish Muscle Development
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One-cell stage zebrafish embryos were injected with 10 pg of a cDNA construct containing a fluorescent protein attached to a PIP-binding protein domain [Bodipy-PI (Echelon Biosciences Inc)]; PLC--PH-GFP (Tobias Meyer Lab, Stanford University, CA) using a Picopump (World Precision Instruments). At 1 dpf, injected zebrafish were incubated in 0.2 mM phenylthiourea to prevent pigment formation. To image, zebrafish were screened for fluorescent myofibers on a macroscope (Zeiss Axio Zoom) and mounted in 1.5% low-melt agarose on a 3 cm glass-bottom petri dish. All confocal images were taken with a Nikon Eclipse Ti confocal microscope using a 40x oilimmersion lens.
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