Total proteins were collected using RIPA buffer (Thermo Scientific, CA, USA) and then quantified for protein quantification by using Pierce™ BCA Protein Quantification Kit (Thermo Scientific) [15 (link)]. Protein (20 μg) was used for electrophoresis loading SDS-PAGE and transferred onto PVDF membranes (Millipore, Billerica, MA, USA). The membranes were blocked with 5% skimmed milk and incubated with primary antibodies, including anti-KIAA1429 (Cell Signaling Technology, 1:1000, #88,358), anti-ROCK2 (Cell Signaling Technology, 1:1000, #47,012), and beta-actin (CWBio, Beijing, China). After incubation by primary antibodies or their corresponding secondary antibodies, blots were developed using SuperSignal West Dura Persistence Substrate (Thermo Scientific).
Anti kiaa1429
Manufactured by Cell Signaling Technology
Sourced in China
Anti-KIAA1429 is a primary antibody product offered by Cell Signaling Technology. It is designed to detect the KIAA1429 protein, which is involved in cellular processes. The antibody can be used for various research applications, such as Western blotting and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Anti rock2, by Cell Signaling Technology (1 mentions)
Beta actin, by CWBIO (1 mentions)
Supersignal west dura persistence substrate, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Pierce bca protein quantification kit, by Thermo Fisher Scientific (1 mentions)
Proteinase inhibitor, by Merck Group (1 mentions)
Ripa reagent, by Merck Group (1 mentions)
Ecl detection system, by Bio-Rad (1 mentions)
Anti pgk1, by Cell Signaling Technology (1 mentions)
2 protocols using anti kiaa1429
1
Protein Quantification and Western Blotting
2
Protein Isolation and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells with the indicated treatment were subjected to protein isolation by RIPA reagents supplemented with 1% proteinase inhibitor (Sigma, USA) and quantified by BCA Protein Assay Kit (Thermo Fisher Scientific). Proteins (∼30 μg) were electrophoresed to 12% SDS-PAGE gels and then transferred to PVDF membranes. Membranes were blocked in BSA (5%) for 2 h and then incubated with primary antibodies (anti-KIAA1429, Cell Signaling Technology, #88,358, 1:1000; anti-PGK1, Cell Signaling Technology, #63,536, 1:1000) overnight at 4 °C. Then, proteins were carefully incubated with secondary antibody for 1 h. Membranes were washed with TBST solution three times. Lastly, signals were detected by ECL detection system (Bio-Rad, California, USA).
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