For determination of protein molecular weights after incubation in media of various pH values, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) using Novex 10% Tris-Glycine mini protein gel (Invitrogen) was applied following manufacturer's instructions. Gels were stained with Coomassie blue (Invitrogen) and de-stained before imaging.
Novex 10 tris glycine mini protein gel
Manufactured by Thermo Fisher Scientific
The Novex 10% Tris-Glycine mini protein gel is a precast polyacrylamide gel designed for the separation and analysis of proteins. The gel has a 10% acrylamide concentration and uses a Tris-glycine buffer system. It is offered in a mini gel format, suitable for small-scale protein electrophoresis applications.
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Lab products found in correlation
2 protocols using novex 10 tris glycine mini protein gel
1
Protein Molecular Weight Determination
2
Protein Size and Molecular Weight Analysis
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Protein size (hydrodynamic radius) of the variable test proteins was determined using the Zetasizer Nano ZS (Malvern instrument) applying the principle of dynamic light scattering. Measurements were done on protein solutions in HEPES buffer pH 7.2 at 37ºC. For determination of protein molecular weights, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) using Novex ® 10% Tris-Glycine mini protein gel (Invitrogen) was applied following manufacturer's instructions. Gels were stained with Coomassie blue (Invitrogen) and de-stained before imaging.
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