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Annexin 5 apc apc

Manufactured by Keygen Biotech
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Sourced in China

Annexin V-APC (APC) is a fluorescently-labeled protein that binds to phosphatidylserine, a cellular component that is exposed on the surface of apoptotic cells. It is commonly used in flow cytometry and other cell-based assays to detect and quantify apoptosis.

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Lab products found in correlation

Propidium iodide, by Keygen Biotech (2 mentions)

Close spelling variants of this product

Annexin V-APC Annexin V-APC/propidium iodide (PI) apoptosis detection kit Annexin V-APC/7-AAD double staining Annexin V-allophycocyanin (APC)/7-aminoactinomycin D (7-AAD) apoptosis detection kit Annexin V-APC/7-AAD apoptosis kit Annexin V-APC/PI Annexin V-APC Apoptosis Detection Kit Annexin V-APC/7-amino-actinomycin D (7-AAD) apoptosis detection kit Annexin V-APC/PI Apoptosis Kit Annexin V-APC/7-AAD Apoptosis Assays Kit

2 protocols using annexin 5 apc apc

1

Apoptosis Detection by Flow Cytometry

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Cells transfected with control shRNA or XRCC1 shRNA were cultured in a 6-well plate. After 48 h, cells were harvested by trypsinization, washed twice with PBS, and then stained with annexin V-APC (APC) (NanJing KeyGen Biotech, Co., Ltd., China) and propidium iodide (PI) (NanJing KeyGen Biotech, Co., Ltd., China) to detect cell apoptosis. Samples were immediately detected in the flow cytometer. This method can distinguish the cells in early (APC+/PI-) and late (APC+/PI+) apoptosis.
Wu Z., Miao X., Zhang Y., Li D., Zou Q., Yuan Y., Liu R, & Yang Z. (2020). XRCC1 Is a Promising Predictive Biomarker and Facilitates Chemo-Resistance in Gallbladder Cancer. Frontiers in Molecular Biosciences, 7, 70.
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2

Apoptosis Quantification by Flow Cytometry

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Cells were cultured in a 6-well plate for 48h, were then collected by trypsinization, and washed twice with PBS. Then, these cells were stained with annexin V-APC (APC) (NanJing KeyGen Biotech, Co., Ltd., China) and propidium iodide (PI) (NanJing KeyGen Biotech, Co., Ltd., China) following the protocol to measure cell apoptosis. At last, these samples were tested in the flow cytometer. This mean was able to identify the cells in early (APC+/PI-) and late (APC+/PI+) apoptosis.
Wu Z., Liu Z., Sun Y., Yuan Y., Zou Q., Wen Y., Luo J, & Liu R. (2023). APEX1 predicts poor prognosis of gallbladder cancer and affects biological properties of CD133+ GBC-SD cells via upregulating Jagged1. Journal of Cancer, 14(8), 1443-1457.
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