1 2 dioleoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl rhod pe

Manufactured by Avanti Polar Lipids

Sourced in United States

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) is a fluorescent lipid probe. It consists of a phosphatidylethanolamine headgroup with a lissamine rhodamine B sulfonyl fluorophore attached to the ethanolamine nitrogen. Rhod-PE can be used to label and visualize lipid membranes and vesicles.

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Lab products found in correlation

8 amino naphtalene 1 3 6 trisulfonic acid sodium salt ants, by Thermo Fisher Scientific (2 mentions) 15n labelled amino acids, by Cambridge Isotopes (2 mentions) Fmoc protected amino acids, by Iris Biotech (1 mentions) P xylene bis pyridinium bromide dpx, by Thermo Fisher Scientific (1 mentions) Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions) Irdye 800cw nhs ester, by LI COR (1 mentions) Peg pbd, by Polymer Source (1 mentions) Peg ppo, by Polymer Source (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (1 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphatidylglycerol, by Avanti Polar Lipids (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Fmoc protected amino acids, by Merck Group (1 mentions) Thioetheramide pc, by Cayman Chemical (1 mentions) Dotap, by Avanti Polar Lipids (1 mentions) Dspe peg2000, by Avanti Polar Lipids (1 mentions) Nbd pc, by Avanti Polar Lipids (1 mentions)

5 protocols using 1 2 dioleoyl sn glycero 3 phosphoethanolamine n lissamine rhodamine b sulfonyl rhod pe

Fluorescent Lipid Labeling Protocol

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Peptide synthesis reagents and Fmoc-protected amino acids were purchased from Iris Biotech (Marktredwitz, Germany) or Merck Biosciences (Darmstadt, Germany). Chemicals for peptide synthesis were obtained from Merck (Darmstadt, Germany) or Biosolve (Valkenswaard, Netherlands), and solvents for HPLC purification were purchased from Fischer Scientific (Geel, Belgium). 4-Chloro-7-nitrobenzofurazan (NBD-Cl) was purchased from VWR (Bruchsal, Germany). The fluorescent probes 8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) and p-xylene-bis-pyridinium bromide (DPX) were obtained from Invitrogen-Molecular Probes (Karlsruhe, Germany). Fluorescently labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) was obtained from Avanti Polar Lipids (Alabaster, AL, USA). The lipids 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), and 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPG) were purchased from NOF (Grobbendonk, Belgium).

Strandberg E., Schweigardt F., Wadhwani P., Bürck J., Reichert J., Cravo H.L., Burger L, & Ulrich A.S. (2020). Phosphate-dependent aggregation of [KL]n peptides affects their membranolytic activity. Scientific Reports, 10, 12300.

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Synthesis and Labeling of Lipids

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¹⁵N-labelled amino acids were purchased from Cambridge Isotope Laboratories (Andover, MA, USA) and were Fmoc-protected using Fmoc-Cl as described previously [33 (link)]. The lipids 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), were purchased from NOF Europe (Grobbendonk, Belgium). The fluorescent lipid 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) were obtained from Avanti Polar Lipids. The fluorescent probes 8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) and p-xylene-bis(pyridinium)bromide (DPX) were obtained from Invitrogen-Molecular Probes (Karlsruhe, Germany).

Wadhwani P., Sekaran S., Strandberg E., Bürck J., Chugh A, & Ulrich A.S. (2021). Membrane Interactions of Latarcins: Antimicrobial Peptides from Spider Venom. International Journal of Molecular Sciences, 22(18), 10156.

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PEG-PBD and PEG-PPO Copolymer Synthesis

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Poly(ethylene glycol) (900)-polybutadiene (1800) copolymer (denoted PEG-PBD, MW 2700 Da) and Poly(ethylene glycol) (450)-poly(propylene oxide) (1400) (denoted PEG-PPO, MW 1850 Da) were purchased from Polymer Source (Dorval, Quebec, Canada). 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(Lissamine rhodamine B sulfonyl) (Rhod-PE) was purchased from Avanti Polar Lipids, Inc. Cu/Zn Superoxide dismutase (SOD; MW 32500) from bovine erythrocytes was from Calbiochem (EMD Millipore, Billerica, MA). Fluorescein isothiocyanate (FITC) was purchased from Thermo Fisher Scientific. IRDye 800CW NHS Ester was purchased from LI-COR, Inc. All other chemicals were used as received. All of the buffer solutions were prepared with deionized water.

Gui T., Luo L., Chhay B., Zhong L., Wei Y., Yao L., Yu W., Li J., Nelson C.L., Tsourkas A., Qin L, & Cheng Z. (2022). Superoxide dismutase-loaded porous polymersomes as highly efficient antioxidant nanoparticles targeting synovium for osteoarthritis therapy. Biomaterials, 283, 121437.

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Peptide Synthesis and Lipid Preparation

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Peptide synthesis reagents and Fmoc-protected amino acids were purchased from Merck Biosciences (Darmstadt, Germany) and/or Iris Biotech (Marktretwitz, Germany). ¹⁵N-labelled amino acids were purchased from Cambridge Isotope Laboratories (Andover, MA, USA) and were Fmoc-protected using Fmoc-Cl as described previously69 . Solvents for peptide synthesis were purchased from Merck (Darmstadt, Germany) or from Biosolve (Valkenswaard, Netherlands) and solvents for HPLC purification were obtained from Fischer Scientific (Geel, Belgium). The lipids 1,2-dierucoyl-sn-glycero-3-phosphatidylcholine (DErPC), 1,2-dierucoyl-sn-glycero-3-phosphatidylglycerol (DErPG), 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine (DMPC), 1,2-dimyristoyl-sn-glycero-3-phosphatidylglycerol (DMPC), 1,2-dimyristoleoyl-sn-glycero-3-phosphatidylcholine (DMoPC), 1,2-dioleoyl-sn-glycero-3-phosphatidylglycerol (DOPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylglycerol (POPG), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rhod-PE) were obtained from Avanti Polar Lipids (Alabaster, USA). The fluorescent probes 8-amino-naphtalene-1,3,6-trisulfonic acid sodium salt (ANTS) and pxylenebis(pyridinium)bromide (DPX) were obtained from Invitrogen - Molecular Probes (Karlsruhe, Germany).

Grau-Campistany A., Strandberg E., Wadhwani P., Reichert J., Bürck J., Rabanal F, & Ulrich A.S. (2015). Hydrophobic mismatch demonstrated for membranolytic peptides, and their use as molecular rulers to measure bilayer thickness in native cells. Scientific Reports, 5, 9388.

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PLA2-Mediated Liposomal Delivery

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Thioetheramide-PC was purchased from Cayman Chemical (Ann Arbor, MI). HSPC, DSPE-PEG2000, DOTAP, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(Lissamine rhodamine B sulfonyl) (Rhod-PE), DSPE-PEG2000-Cy7, and NBD-PC were purchased from Avanti Polar Lipids Inc. sPLA₂ enzyme from Naja mossambica was purchased from Sigma-Aldrich Co. Rabbit polyclonal antibody to sPLA₂-IIa, p-P65, and p-P100 antibodies were purchased from Abcam. Bovine synovial fluid was obtained from Lampire Biological Laboratories Inc. All other chemicals were used as received. All of the buffer solutions were prepared with deionized water.

Wei Y., Yan L., Luo L., Gui T., Jang B., Amirshaghaghi A., You T., Tsourkas A., Qin L, & Cheng Z. (2021). Phospholipase A2 inhibitor–loaded micellar nanoparticles attenuate inflammation and mitigate osteoarthritis progression. Science Advances, 7(15), eabe6374.

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