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Arachidonic acid

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Arachidonic acid is a long-chain polyunsaturated fatty acid. It is a core component of cellular membranes and serves as a precursor for the synthesis of various bioactive lipid mediators.

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Lab products found in correlation

Arachidonic acid, by Merck Group (3 mentions) Clariostar plate reader, by BMG Labtech (3 mentions)

3 protocols using arachidonic acid

1

Bacterial growth modulation by WTA and AA

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Overnight cultures of JE2, tcaA, and lcpA mutants were pelleted, and the supernatant saved. Growth inhibition assays were subsequently performed by adding 20 µl of OD600nm 0.1 bacterial suspension to 180 µl 10% overnight supernatant diluted in fresh MHB++ containing 400 μM of arachidonic acid (Sigma). Purified WTA extracts were also added to the growth media at a final concentration of 2%. The ability of the bacteria to survive the arachidonic acid was determined by quantifying bacterial growth (OD600nm) following 24 hr at 37°C using a CLARIOstar plate reader (BMG Labtech).
Douglas E.J., Palk N., Brignoli T., Altwiley D., Boura M., Laabei M., Recker M., Cheung G.Y., Liu R., Hsieh R.C., Otto M., O'Brien E., McLoughlin R.M, & Massey R.C. (2023). Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia. eLife, 12, RP87026.
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2

Bacterial growth modulation by WTA and AA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Overnight cultures of JE2, tcaA, and lcpA mutants were pelleted, and the supernatant saved. Growth inhibition assays were subsequently performed by adding 20 µl of OD600nm 0.1 bacterial suspension to 180 µl 10% overnight supernatant diluted in fresh MHB++ containing 400 μM of arachidonic acid (Sigma). Purified WTA extracts were also added to the growth media at a final concentration of 2%. The ability of the bacteria to survive the arachidonic acid was determined by quantifying bacterial growth (OD600nm) following 24 hr at 37°C using a CLARIOstar plate reader (BMG Labtech).
Douglas E.J., Palk N., Brignoli T., Altwiley D., Boura M., Laabei M., Recker M., Cheung G.Y., Liu R., Hsieh R.C., Otto M., O'Brien E., McLoughlin R.M, & Massey R.C. (2023). Extensive remodelling of the cell wall during the development of Staphylococcus aureus bacteraemia. eLife, 12, RP87026.
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3

Arachidonic Acid Growth Inhibition Assay

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Overnight cultures of JE2, tcaA, and lcpA mutants were pelleted, and the supernatant saved. Growth inhibition assays were subsequently performed by adding 20 μl of OD600nm 0.1 bacterial suspension to 180 μl 10% overnight supernatant diluted in fresh MHB++ containing 400μM of arachidonic acid (Sigma). Purified WTA extracts were also added to the growth media at a final concentration of 2%. The ability of the bacteria to survive the arachidonic acid was determined by quantifying bacterial growth (OD600nm) following 24 h at 37°C using a CLARIOstar plate reader (BMG Labtech).
Douglas E.J., Palk N., Brignoli T., Altwiley D., Boura M., Laabei M., Recker M., Cheung G.Y., Liu R., Hsieh R.C., Otto M., O’Brien E., McLoughlin R.M, & Massey R.C. (2023). Extensive re-modelling of the cell wall during the development of Staphylococcus aureus bacteraemia. bioRxiv.
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