Sixth instar larvae of the greater wax-moth G. mellonella (Livefoods Direct Ltd, Sheffield, England), were stored in the dark at 15 °C to prevent pupation. Larvae weighing 0.22 ± 0.03 g were selected and used within two weeks of receipt. Ten healthy larvae per treatment and controls (n = 3) were placed in sterile nine cm Petri dishes lined with Whatman filter paper and containing some wood shavings. Larvae were inoculated with yeast cells or β - glucan through the last left pro-leg into the hemocoel with a Myjector U-100 insulin syringe (Terumo Europe N.V., Belgium). Larvae were acclimatized to 30 °C for 1 hour prior to all experiments and incubated at 30 °C for all studies. All experiments were performed independently on three separate occasions.
Myjector u 100 insulin syringe
Manufactured by Terumo
Sourced in Belgium
The Myjector U-100 insulin syringe is a medical device designed for the administration of U-100 insulin. It features a pre-attached needle and is calibrated in 1/100 increments for precise dosing.
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Lab products found in correlation
9 protocols using myjector u 100 insulin syringe
1
Assessing Immune Response in Wax Moth Larvae
2
Insect Infection Model with S. aureus
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Ten larvae of G. mellonella were stored in the dark at 15°C. Larvae of the same age and weighing around 0.2 g were inoculated with 20 µL of water containing 5 10 7 S. aureus cells through the last pro-leg using a Myjector U100 insulin syringe (Terumo Europe, Leuven, Belgium).
3
Wax Moth Larvae Infection Model
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Sixth instar larvae of the greater wax-moth G. mellonella (Livefoods Direct Ltd., Sheffield, England), were stored in the dark at 15°C and maintained on wood chippings. 21, 37 Larvae weighing 0.22 -0.03 g were selected and used within 2 weeks of receipt. Ten healthy larvae per treatment and controls were placed in sterile 9-cm Petri dishes lined with Whatman filter paper and containing some wood shavings. Larvae were inoculated with drug and/or bacterial pathogen through the last left pro-leg into the hemocoel with a Myjector U-100 insulin syringe (Terumo Europe N.V., Belgium). Larvae were acclimatized to 37°C for 1 hour before all experiments and incubated at 37°C for all studies. All experiments were performed independently on three separate occasions.
4
Liver Radioembolization Tracer Protocol
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An embolization setup in the liver was performed to mimic the clinical setup for liver radioembolization.24 For this purpose, animals were anesthetized by intraperitoneal injection of a mixture containing Hypnorm (Vetapharma, Leeds, United Kingdom), dormicum (Roche, Basel, Switzerland), and water (1:1:2). After shaving and cleaning with ethanol (70%), the abdominal cavity was incised for 0.5 cm and the spleen was exposed. Of the 99mTc-MAA-Ad solution (2 mg/mL), 100 μL was injected into the spleen using a Myjector U-100 insulin syringe (29G x ½” 0.33 x12 mm, Terumo Europe, Leuven, Belgium) and after 5 s the needle was removed and the spleen was repositioned in the peritoneal cavity. The incision was sutured by 2-4 stitches and the animals were placed under a heating lamp to maintain the body temperature at 37 oC. At 2 h after injection, the organ distribution of the tracer in mice was imaged using SPECT and quantitated with biodistribution studies as described above (Figure 3 C, D).
5
Haemocoel Injection of Biomolecules in G. mellonella
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The G. mellonella haemocoel was injected with 20 μl of selected biomolecular solution using a 0.3 ml Terumo® Myjector® U-100 insulin syringe through the base of the last proleg. Three control groups of ten larvae were injected with sterile deionised H2O. Larvae were incubated at 30°C for 24 h. Larvae were assessed visually for viability 24 h after injection with plasma treated biomolecule solutions and percentage survival was noted. Larvae were considered dead if they were unmoving, failing to reorient themselves if placed on their backs or failed to respond to stimuli [59 (link)].
6
Pathogenicity Assay of Pseudomonas Isolates
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In the pathogenicity studies, ATCC 27853, PAO1, CF1, CF2, and CF3 were investigated by preparing a dilution series (3 × 100 to 3 × 105 CFU/mL) of each isolate, and ten larvae were inoculated through the last left pro-leg into the hemocoel using a Myjector U-100 insulin syringe (Terumo Europe NV, Leuven, Belgium) with 20 μL of washed cultures. Undisturbed larvae and larvae inoculated with PBS were utilized as controls. The injected larvae were placed in petri dishes containing wood shavings and were incubated at 37 °C. Mortality, cuticle discoloration, and response to touch were recorded 96 h post-injection. All experiments were performed independently on three separate occasions.
7
Insect Infection Model with S. aureus
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Ten larvae of G. mellonella were stored in the dark at 15°C. Larvae of the same age and weighing around 0.2 g were inoculated with 20 µL of water containing 5 10 7 S. aureus cells through the last pro-leg using a Myjector U100 insulin syringe (Terumo Europe, Leuven, Belgium).
8
Galleria mellonella Infection Model
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Sixth instar larvae of the greater wax-moth G. mellonella (Livefoods Direct Ltd., Sheffield, England), were stored in the dark at 15 °C to prevent pupation. Larvae weighing 0.22 ± 0.03 g were selected and used within 2 weeks of receipt. Ten healthy larvae per treatment and controls (20 μl PBS for appropriate incubation time); (n = 3) were placed in sterile 9 cm Petri dishes lined with Whatman filter paper and containing wood shavings. Larvae were inoculated with viable or non-viable (heat-killed) conidia through the last left pro-leg into the hemocoel with a Myjector U-100 insulin syringe (Terumo Europe N.V., Belgium). Larvae were acclimatized to 37 °C for 1 h prior to all experiments and incubated at 37 °C for all studies. All experiments were performed independently on three separate occasions.
9
Galleria mellonella Larval Infection Model
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Sixth instar larvae of G. mellonella (Mealworm Company, Sheffield, England) were stored in the dark at 15 °C in wood shavings prior to use. Larvae were stored immediately upon receipt from the supplier. Larvae that had been stored for 1-3 weeks and weighing 0.24-0.28 g were used. Larvae were inoculated with 20 µl of culture filtrate through the last pro-leg using a Myjector U100 insulin syringe (Terumo Europe, Leuven, Belgium). Control larvae were inoculated with 20 µl of Sabouraud dextrose liquid medium. Larvae were incubated at 20 °C.
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