White star phacoemulsification system

The crystalline lenses of rabbits were extracted by means of a standard phacoemulsification technique using the White Star phacoemulsification system (Abbott Medical Optics, Santa Ana, CA, USA) [37 (link)]. Surgeries were performed through 2.8-mm clear corneal incisions. To achieve mydriasis, tropicamide 1% (Alcon Laboratories, Geneva, Switzerland) and phenylephrine hydrochloride 2.5% (Alcon Laboratories, Geneva, Switzerland) eye drops were administered approximately 30 min before lens extraction surgery. Corneal incisions were closed with 10/0 nylon sutures, and the rabbits were left aphakic with an intact posterior capsule for at least one week before the experimental cell-injection procedures.

The crystalline lenses of rabbits were extracted using a standard phacoemulsification technique as previously described^{17 (link)}. Mydriasis was achieved by the administration of tropicamide 1% (Alcon Laboratories, Texas, USA) and phenylephrine hydrochloride 2.5% (Alcon Laboratories) eye drops approximately 30 minutes before surgery. A clear corneal incision was made with a 2.8 mm disposable keratome. A 5.0 mm diameter continuous curvilinear capsulotomy of the anterior capsule was created under viscoelastic material (Viscoat; Alcon Laboratories) instilled into the anterior chamber. Hydro-dissection was performed using a 27-gauge cannula. The lens was then aspirated and removed with a standard phacoemulsification procedure using the White Star phacoemulsification system (Abbott Medical Optics, California, USA). Subsequently, the corneal incision was sutured with 10/0 nylon suture and the rabbits were left aphakic with an intact posterior capsule for at least one week before the CE-CI procedures. Cataract extraction surgery of the rabbits were performed by either HSO or FMW.

The crystalline lenses of rabbits were extracted using a standard phacoemulsification technique as described^{53 (link)}. To achieve mydriasis, tropicamide 1% (Alcon Laboratories, Texas, USA) and phenylephrine hydrochloride 2.5% (Alcon Laboratories) eye drops were administered approximately 30 minutes before surgery. A clear corneal incision was made with a 2.8 mm disposable keratome. A 5.0 mm diameter continuous curvilinear capsulotomy of the anterior capsule was created under viscoelastic material (Viscoat; Alcon Laboratories) instilled into the anterior chamber. Hydro-dissection was performed using a 27-gauge cannula. The lens was then aspirated and removed with a standard phacoemulsification procedure using the White Star phacoemulsification system (Abbott Medical Optics, California, USA). Subsequently, the corneal incision was sutured with 10/0 nylon suture and the rabbits were left aphakic with an intact posterior capsule for at least one week before the cell-based procedures.