Human MM cell lines NCI-H929, LP-1, RPMI-8226, and U266 were used in this study. NCI-H929 was obtained from Dr. Margaret HL Ng (Prince of Wales Hospital, Chinese University of Hong Kong). LP-1-secreting IgG l light chain was kindly provided by Dr. Hallek (Laboratorium für Molekulare Biologie, Genzentrum, Ludwig-Maximilians-Universität München, Germany). RPMI-8226 (CCL-155) and U266 (TIB-196) were obtained from American Type Culture Collection (ATCC). The cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 2 mM of L-glutamine (Sigma, St. Louis, MO, USA), 100 U/ml of penicillin G, and 100 mg/ml of streptomycin (Sigma). For depletion of URI, plasmids containing shURI and lentivirus-shURI and control virus were purchased from Genechem Co. (Shanghai, China). MM cells were seeded into wells. After incubating for 24 h, these cells were transfected with plasmid or lentivirus according to the manufacturer's protocol.
Rpmi 8226 ccl 155
Manufactured by American Type Culture Collection
RPMI-8226 (CCL-155) is a cell line derived from a human myeloma patient. It is a suspension cell line that can be used for research purposes.
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2 protocols using rpmi 8226 ccl 155
1
Depletion of URI in MM Cell Lines
2
Myeloma Cell Lines and Patient Samples
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Myeloma cell lines ARP-1, H929 and U266 were provided by Dr. Zhiqiang Liu's lab of Tianjin Medical University. The MCF7 (#HTB-22), HEK293T (#ACS-4500), RPMI8226 (CCL-155), and MM.1S (CRL-2974) cells were purchased from the American Type Culture Collection. Primary myeloma cells were isolated from bone marrow aspirates of myeloma patients by using anti-CD138 antibody-coated magnetic beads (Miltenyi Biotec). Myeloma cells were maintained in RPMI1640 medium with 10% fetal bovine serum (FBS). MCF7 and HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% FBS. Patient samples were obtained from the Qingdao Municipal Hospital of Qingdao University and The First Affiliated Hospital of Xiamen University. Bone lesions in the study participants were characterized by radiologists at Qingdao Municipal Hospital. This study was approved by the Ethics Committee of Xiamen University, and all protocols conformed to the Ethical Guidelines of the World Medical Association Declaration of Helsinki.
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