Loading buffer

The LAMP reagent containing Bst 2.0 WarmStart DNA polymerase, 10 × isothermal amplification buffer, 100 mM MgSO₄ and dNTPs, was purchased from New England BioLabs (Ipswich, MA, USA). Primers, plasmid DNA (pDNA) of E. coli O157:H7, Salmonella spp. and S. aureus, and agarose powder were obtained from Sangon (Shanghai, China). MnCl₂ and Calcein (CAS No.: 154071–48-4, C₃₀H₂₆N₂O₁₃, 2,2′,2″,2″′-[(3′,6′-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9′-xanthene]-2′,7′-diyl)bis (methanediylnitrilo)] tetraacetic acid) were purchased from Macklin (Shanghai, China) and Sigma-Aldrich (St. Louis, MO, USA), respectively. PDMS prepolymer (Sylgard 184) and curing agent were obtained from Dow Corning. Whatman filter paper grade 1 and Gel-Red was purchased from Beyotime Biotechnology (Shanghai, China). The Wizard genomic DNA purification kit was obtained from Promega (Madison, WI, USA). S. aureus real-time PCR kit was purchased from Zeye Biotechnology (Shanghai, China). A 100 bp DNA size marker was obtained from Sparkjade Biotechnology (Shandong, China). Loading buffer was purchased from Dingguo Biotechnology (Beijing, China). 50 × TAE buffer was purchased from YuanYe Biotechnology (Shanghai, China). Clinx Gel imaging system (Shanghai, China) was used to detect the target bands.