MCF-7 and MDA-MB-231 cells were transfected with miR-320d mimics for 24 h. After wash with ice-cold PBS, cells were resuspended in annexin V binding buffer and incubated with FITC-conjugated annexin V/PI (ThermoFisher, USA) for 15 min at room temperature. The cells were then analyzed with CytoFLEX system (Beckman, USA).
Fitc conjugated annexin 5 pi
Manufactured by Thermo Fisher Scientific
Sourced in United States
FITC-conjugated annexin V/PI is a fluorescent labeling reagent used in flow cytometry applications. Annexin V has a high affinity for phosphatidylserine, a lipid that translocates to the outer cell membrane during apoptosis. FITC (fluorescein isothiocyanate) is covalently conjugated to annexin V, allowing for the detection of apoptotic cells. Propidium iodide (PI) is a DNA-binding dye that is used to identify necrotic or late-stage apoptotic cells. The combined use of FITC-annexin V and PI enables the discrimination of viable, early apoptotic, and late apoptotic/necrotic cell populations.
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2 protocols using fitc conjugated annexin 5 pi
1
Annexin V/PI Apoptosis Assay
2
Evaluating Apoptosis in A549 and H460 Cells
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A549 and H460 cells were transfected with pcDNA3.1-MIER3 for 24 hours. The cells were washed with ice-cold PBS before being resuspended in Annexin V to facilitate binding and being incubated at room temperature for 15 minutes using FITC-conjugated Annexin V/PI (ThermoFisher, USA). An Attune NxT Flow Cytometer (ThermoFisher Scientific, USA) was then used to analyze the cells.
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