Modular d2400

Following a 12-hour overnight fast, blood samples were obtained from the antecubital vein of each subject by venipuncture and were immediately centrifuged, aliquoted, and frozen at -20℃. The frozen serum and plasma samples were stored at -80℃ until analysis. Fasting serum glucose, total cholesterol, triglyceride, and HDL-cholesterol (HDL-C) levels were measured by enzymatic methods using a Hitachi Modular D2400 automated chemistry analyzer (Hitachi, Tokyo, Japan). Levels of low-density lipoprotein-cholesterol (LDL-C) were calculated using the following formula: LDL-C=total cholesterol-HDL-C-(triglyceride/5). Serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured. Fasting insulin levels were measured using a chemiluminescent microparticle immunoassay (Abbott Architect system, Irving, TX, USA). Insulin resistance was estimated using the homeostatic model assessment of insulin resistance (HOMA-IR) and calculated using the following formula: HOMA-IR=[fasting insulin (µIU/mL)×fasting glucose (mg/dL)/18]/22.522) (link).

After a 12-hour overnight fast, blood specimens were attained from the antecubital vein of the participants by venipuncture and were promptly centrifuged, aliquoted, and frozen at -20°C. The frozen serum and plasma samples were accumulated at -80°C until final analysis. Fasting plasma glucose, total cholesterol, triglyceride, and high-density lipoprotein-cholesterol (HDL-C) levels were quantified by enzymatic methods using a Hitachi Modular D2400 automated chemistry analyzer (Hitachi, Tokyo, Japan). Levels of low-density lipoprotein-cholesterol (LDL-C) were determined using the following equation: LDL-C=total cholesterol − HDL-C − (triglyceride/5). Fasting insulin concentrations were assessed using a chemiluminescent microparticle immunoassay (Abbott Architect System, Irving, TX, USA). We also subjected the fasting data to various transformations and ultimately defined quantitative insulin sensitivity check index (QUICKI=1/[log(I₀)+log(G₀)]), where I₀ is the fasting insulin, and G₀ is the fasting glucose. Insulin resistance was computed using the homeostatic model assessment of insulin resistance (HOMA-IR) and assessed using the following formula: HOMA-IR=(fasting insulin [μIU/mL]×fasting glucose [mg/dL]/18)/22.5 [16 (link)].