Eg1160 embedding station

Manufactured by Leica camera

Sourced in Germany

The EG1160 is an embedding station designed for use in histological and cytological laboratories. It facilitates the embedding of biological samples in paraffin blocks, a crucial step in the preparation of tissue samples for microscopic analysis. The equipment allows for the controlled heating and cooling of paraffin to ensure proper embedding of the specimen.

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Lab products found in correlation

Leica tp1020, by Leica Microsystems (1 mentions) Leica hi1210, by Leica Biosystems (1 mentions) Rm2135, by Leica camera (1 mentions) Ventana benchmark xt machine, by Roche (1 mentions) Asp300s tissue processor, by Leica camera (1 mentions)

4 protocols using eg1160 embedding station

Tissue Fixation and Sectioning Protocol for Histological Analysis

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Embryos and reproductive tissues from neonates or adults were dissected and fixed in 4% PFA at 4°C overnight. Fixed tissues were further processed for cryo- or paraffin sectioning. For cryosectioning, tissues were washed 3 times in PBS and immersed in 30% sucrose/PBS solution overnight at 4°C. Tissues were then incubated in 30% sucrose/O.C.T. (Tissue-Tek) at room temperature for 1 hour and frozen blocks were made with the same sucrose/O.C.T. mixture. Frozen sections were cut at 10 μm and stored at −80°C for further histological analysis and immunofluorescence.
For paraffin sectioning, fixed tissues were rinsed 3 times in PBS and stored in 70% ethanol. Serial dehydration and paraffin infiltration of fixed tissues were performed using a tissue processor, Leica TP1020 (Leica Microsystems NusslochGmbh, Heidelberger). Paraffin blocks were made using a Leica EG1160 embedding station. Paraffin sections were cut at 4 μm and store at 4°C for histological analysis and TUNEL assay.

Huang C.C., Orvis G.D., Kwan K.M, & Behringer R.R. (2014). Lhx1 is required in Müllerian duct epithelium for uterine development. Developmental biology, 389(2), 124-136.

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Placental Tissue Processing and Preservation

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In the primary study, a segment from the central region of each placenta was cautiously excised, avoiding areas with macroscopic infarction and immediately fixed in 10% buffered formaldehyde solution. The placental tissue was dehydrated using an ascending ethanol series, cleared with xylene, and infiltrated with paraffin wax using an automated tissue processor (Sakura 5, Torrance, California, USA). The tissue was then embedded in an embedding station (Leica EG 1160 embedding station, Germany) and stored until required. Prior to the immunostaining procedure, the paraffin wax-embedded blocks were trimmed and cut into 3 μm sections using a rotatory microtome (Leica RM2135, UK). Sections were then floated on a 50 °C water bath (Leica HI1210, Leica Biosystems, UK), collected onto X-tra adhesive coated slides (Leica Biosystems, UK), and heat fixed on a 60 °C hot plate (Sakura, USA) overnight.

Naidoo N., Abel T., Moodley J, & Naicker T. (2023). Immunoexpression of neuropilin-1 in the chorionic villi of HIV-infected preeclamptic South African women of African ancestry. Histochemistry and Cell Biology.

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Histological Analysis of Skin Samples

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The explants were fixed for 24 h in buffered formalin, dehydrated and then impregnated in paraffin using a Leica PEARL dehydration automat. The samples were embedded using a Leica EG 1160 embedding station. Next, 5‐μm‐thick sections were made using a Leica RM 2125 Minot‐type microtome, and the sections were mounted on Superfrost^® histological glass slides. The tissue morphology of the epidermal and dermal structures was assessed through microscopic observation of formalin‐fixed paraffin‐embedded (FFPE) skin sections after Masson's trichrome staining, Goldner variant. The degree of staining was assessed by microscopic observation.

Majewski G.P., Singh S, & Bojanowski K. (2021). Olive leaf‐derived PPAR agonist complex induces collagen IV synthesis in human skin models. International Journal of Cosmetic Science, 43(6), 662-676.

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Collagen I Immunohistochemistry in Paraffin Sections

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AEHTs were fixed with 4% buffered formalin and processed for paraffin embedding. After embedding in paraffin using a Leica ASP300S tissue processor with a Leica EG1160 embedding station, sections (4 µm) were processed for immunohistochemistry as follows: After dewaxing and inactivation of endogenous peroxidases (PBS/3% hydrogen peroxide), antibody-specific antigen retrieval was performed using the Ventana Benchmark XT machine (Ventana, Tuscon, Arizona). Sections were blocked and afterward incubated with the primary antibody against Collagen I (Col1A1; sc-8783; Santa Cruz). Sections were incubated with primary antibody for 1 hour, then, the antigoat Histofine Simlpe Stain MAX PO immune-enzyme polymer (#414161F, medac GmbH, Wedel, Germany) was used as secondary antibody. Detection of secondary antibodies and counter staining was performed with an ultraview universal 3,3′-Diaminobenzidine detection kit from (Ventana, Tuscon, Arizona). Staining was evaluated in a blinded fashion. Representative pictures were taken with a digital Leica DMD109 microscope.

Lemoine M.D., Lemme M., Ulmer B.M., Braren I., Krasemann S., Hansen A., Kirchhof P., Meyer C., Eschenhagen T, & Christ T. (2020). Intermittent Optogenetic Tachypacing of Atrial Engineered Heart Tissue Induces Only Limited Electrical Remodelling. Journal of cardiovascular pharmacology, 77(3).

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