Cephalotin

Out of 360 ATMs at four locations in Hamadan (number provided by the central bank of the islamic republic of Iran) 96 ATMs were randomly selected. Using a sterile swab soaked in saline, samples were taken from the surfaces of the ATM keyboards. The swab was put into nutrient broth media and transferred to the microbiology laboratory of the University of Medical Sciences, Hamadan and incubated for 30 min. In order to differentiate microorganisms, swabs were cultured on blood agar and MacConkey agar plates and incubated at 37°C for 18–24 hours. Identification of the isolated bacteria was performed using standard microbiological methods [16 ]. For all isolated strains, antibacterial susceptibility was tested using the standard Kirby-Bauer disk agar diffusion (DAD) method on Mueller Hinton agar (Merk Co., Germany) according to the clinical and laboratory standards institute guidelines (CLSI; 2015, M100-S25) [17 (link)] using gentamicin (10 µg), vancomycin (30 µg), trimethoprim/sulfamethoxazole (25 µg), amikacin (30 µg), tobramycin (10 µg), cephalotin (30 µg), norfloxacin (5 µg), and ceftizoxim (30 µg) disks (Mast Co.UK).
All statistical analyses were performed using the SPSS software package, version 21.