Male Crl:WI (Han) rats and Crl:CD1 (ICR) mice (6–8 weeks) from Charles River Laboratories (Lyon, France) were acclimatised for 1 week. Six animals were housed per cage, on a 12 h light/dark cycle, at constant temperature (22 ± 2 °C). Standard food and tap water were provided ad libitum. Care of animals was undertaken in compliance with the European Community Directive 86/609/CEE for the use of laboratory animals and with the Autonomous Catalan law (Decret 214/1997). All experimental procedures were approved by the Almirall Ethics Committee.
Crl wi han rats
Manufactured by Charles River Laboratories
Sourced in France
Crl:WI (Han) rats are a commonly used laboratory animal model. They are genetically defined and well-characterized inbred strain of Wistar rats developed by Charles River Laboratories. These rats are widely used in biomedical research due to their well-established physiological and behavioral characteristics.
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3 protocols using crl wi han rats
1
Rodent Acclimatization and Ethical Procedures
2
Timed-Pregnant Rats for In Utero Exposures
3
Wistar Rat Catheterization Protocol
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One hundred Wistar (aged 9 weeks): Crl:WI (Han) rats were obtained from Charles River Laboratories (Domain des Oncins, France). The rats had an acclimatization period of at least 14 days before start of the experiments.
During the acclimatization period, a polyurethane catheter was implanted into the posterior vena cava via the left femoral 1 Fresenius Kabi Deutschland GmbH, Bad Homburg, Germany 2 Charles River SAS, Saint-Germain-Nuelles, France vein. Following implantation, the animals were maintained on continuous infusion with physiological saline prior to the start of treatment. All animals were housed singly in a temperature-controlled room with a 12-hour light/dark cycle. The room temperature and relative humidity were maintained in the ranges of 22 C + 3 C and >35% humidity. All animals had access to food (diet reference A04C-10, obtained from Safe diets, Augy, France) and water ad libitum. The body weights of the animals at the start of infusion ranged from 164 to 294 g. The study was approved by the ethical committee of Charles River and authorized by the French authorities (Ministère de l'Enseignement supérieur, de la Recherche et de l'Innovation).
During the acclimatization period, a polyurethane catheter was implanted into the posterior vena cava via the left femoral 1 Fresenius Kabi Deutschland GmbH, Bad Homburg, Germany 2 Charles River SAS, Saint-Germain-Nuelles, France vein. Following implantation, the animals were maintained on continuous infusion with physiological saline prior to the start of treatment. All animals were housed singly in a temperature-controlled room with a 12-hour light/dark cycle. The room temperature and relative humidity were maintained in the ranges of 22 C + 3 C and >35% humidity. All animals had access to food (diet reference A04C-10, obtained from Safe diets, Augy, France) and water ad libitum. The body weights of the animals at the start of infusion ranged from 164 to 294 g. The study was approved by the ethical committee of Charles River and authorized by the French authorities (Ministère de l'Enseignement supérieur, de la Recherche et de l'Innovation).
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