The activity of β-hexosaminidase A and cathepsin D was measured in 4-5 μg of protein cell lysate from the cortex and hippocampus at 8 wk and 20 wk of age (n> 5 for each group) in a 96-well plate. To measure β-hexosaminidase A activity, 3.2 mM 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide (MUGS) potassium salt (Santa Cruz Biotechnology, Santa Cruz, CA) was added to the cell lysate and incubated for 1 hr at 37°C. Next, 2-amino-2-methyl-1-propanol (0.1 M) was added and fluorescence was read immediately with a 355 nm excitation filter and 460 nm emission filter using a Fluoroskan Ascent II plate reader (LabSystems, Helsinki). Cathepsin D activity was assessed using the cathepsin D activity assay kit (BioVision, Mountain View, CA) according to the manufacturer's instructions. Fluorescence was read with a 320 nm excitation filter and 460 nm emission filter.
4 methylumbelliferyl 6 sulfo n acetyl β d glucosaminide mugs potassium salt
Manufactured by Santa Cruz Biotechnology
4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide (MUGS) potassium salt is a fluorogenic substrate used in the detection and quantification of N-acetyl-β-D-glucosaminidase (NAG) activity. NAG is an enzyme that catalyzes the hydrolysis of N-acetyl-β-D-glucosaminides. The cleavage of the MUGS substrate by NAG results in the release of a fluorescent 4-methylumbelliferone product, which can be measured to determine the NAG activity in a sample.
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Lab products found in correlation
2 protocols using 4 methylumbelliferyl 6 sulfo n acetyl β d glucosaminide mugs potassium salt
1
Enzymatic Activities in Cortex and Hippocampus
2
Enzymatic Activity Profiling in Brain Regions
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The activity of β-hexosaminidase A and cathepsin D was measured in 4–5 μg of protein cell lysate from the cortex and hippocampus at 8 and 20 weeks of age (n ≥ 5 for each group) in a 96-well plate. To measure β-hexosaminidase A activity, 3.2 mM 4-methylumbelliferyl-6-sulfo-N-acetyl-β-D-glucosaminide (MUGS) potassium salt (Santa Cruz Biotechnology, Santa Cruz, CA) was added to the cell lysate and incubated for 1 h at 37 °C. Next, 2-amino-2-methyl-1-propanol (0.1 M) was added, and fluorescence was read immediately with a 355-nm excitation filter and 460-nm emission filter using a Fluoroskan Ascent II plate reader (LabSystems, Helsinki). Cathepsin D activity was assessed using the cathepsin D activity assay kit (BioVision, Mountain View, CA) according to the manufacturer’s instructions. Fluorescence was read with a 320-nm excitation filter and 460-nm emission filter.
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