Anti c myc

All constructs were confirmed by DNA sequencing. pTKN101 (pVenus-GBF1) has been described previously^{30 (link)}. pCMS-EGFP-Arf1-T31N (pArf1-T31N-GFP) and pCMS-EGFP-Arf1-Q71L (pArf1-Q71L-GFP) were constructed by insertion of Arf1 mutant cDNAs into pCMS-EGFP (Clontech, Invitrogen) using BamHI and AgeI restriction enzyme sites, placing the mutant Arf proteins under control of the SV40 promoter. pRK5-myc-Miro1 (Plasmid 47888), pRK5-myc-Miro2 (Plasmid 47891) and pAc-GFP-Sec61b (Plasmid 15108) were purchased from Addgene (Cambridge, MA, USA). GFP-3x-Nup160 was a gift from Valérie Doye, Institut Jacques Monod - UMR7592 CNRS Université Paris-Diderot, Paris, France, and has been described^{44 (link)}. Cricetulus griseus (hamster) GBF1 was a gift of Paul Melançon (University of Alberta, Edmonton, Canada)^{45 (link)}. The following mouse monoclonal antibodies were used: anti-GBF1 (BD Biosciences, Franklin Lakes, NJ, USA, Cat. No 612116), anti-Miro1 (Sigma-Aldrich, St Louis, MO, USA, Cat. No SAB1407648), anti-α-tubulin (GeneTex, Irvine, CA, USA, Cat. No GTX628802), anti-γ-tubulin (Sigma-Aldrich, Cat. No T6557), anti-c-myc (Biolegend, San Diego, CA, USA, Cat. No 626802) and anti-GFP (Roche Diagnostics, Indianapolis, IN, USA, Cat. No 11814460001). Rabbit polyclonal antibodies against Miro2 (Cat. No HAP012624) and PTPIP51/RMDM3 (Cat. No HPA009975) were purchased from Sigma-Aldrich.