The following antibodies were used: mouse Anti-p53 (Ab6) (Calbiochem OP43); rabbit anti-phospho-p53 (Ser15) (Cell Signaling 9284); mouse anti-phospho-Histone H2A.X (Ser139) (Millipore 05-636); rabbit anti-Histone H2A.X (Millipore 07-627); rabbit anti-acetyl-Histone H3 (Lys9) (Millipore 07-352); rabbit anti-Histone H3 (K4me1) (abcam ab8895); rabbit anti-Histone H3 (K27Ac) (abcam ab4729); rabbit anti-Histone H3, CT, pan (Millipore 07-690); rabbit anti-GAPDH (14C10) (Cell Signaling 2118).
Rabbit anti acetyl histone h3 lys9
Manufactured by Merck Group
Rabbit anti-acetyl-Histone H3 (Lys9) is a laboratory reagent used for the detection of acetylated histone H3 at lysine 9 (H3K9ac) in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry. It is a polyclonal antibody raised in rabbits against a synthetic peptide corresponding to the acetylated lysine 9 residue of histone H3.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti phospho p53 ser15, by Cell Signaling Technology (1 mentions)
Rabbit anti gapdh 14c10, by Cell Signaling Technology (1 mentions)
Mouse anti phospho histone h2a x ser139, by Merck Group (1 mentions)
Rabbit anti histone h3 k27ac, by Abcam (1 mentions)
Rabbit anti vip, by Immunostar (1 mentions)
Rabbit anti p300, by Santa Cruz Biotechnology (1 mentions)
Mouse anti neun, by Merck Group (1 mentions)
2 protocols using rabbit anti acetyl histone h3 lys9
1
Antibody Characterization for Research
2
Epigenetic Regulation of Neural Circuits
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The mice were euthanized, and the brain tissue was harvested as indicated by the conditions. Immunofluorescence staining, western blotting and chromatin immunoprecipitation were performed as previously described (Lee et al., 2015 (link); Shi et al., 2013 (link); Xu et al., 2007 (link)). The antibodies used were the following: mouse anti-ZBTB20 9A10 (1:1,000, home made), mouse anti-BMAL1 (1:1,000, home made), goat anti-PROKR2 (Santa Cruz Biotechnology, 1:400, Cat# sc-54317 RRID:AB_2253158 ), rabbit anti-AVP (Millipore,1:4000, Cat# AB1565 RRID:AB_11212336 ); rabbit anti-VIP (Immunostar, 1:2000, Cat# 20077 RRID:AB_572270 ); rabbit anti-P300 (Santa Cruz Biotechnology, 1:400, Cat# sc-584 RRID:AB_2293429 ); mouse anti-NeuN (Millipore, 1:100, Cat# MAB377 RRID:AB_2298772 ); goat anti-GAD65/67 (Santa Cruz Biotechnology, 1:400, Cat# sc-7513 RRID:AB_2107745 ); rabbit anti-acetyl-histone H3 (Lys9) (Millipore, 1:500, Cat# 07–352 RRID:AB_310544 ); and rabbit anti-dimethyl-histone H3 (Lys9) (Upstate Biotechnology, 1:500, Cat# 07–441 RRID:AB_310619 ). The NIH Image J (RRID:SCR_003070 ) software was used for the quantification of the band from Western blots. The primer sequences for CHIP are provided in Figure 5—source data 1 .
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