A total of 25 males and females mice were used from wild-type (C57BL/6J, n = 15), PV-Cre (B6;129P2-Pvalbtm1(cre)Arbr/J; Jackson Labs, n = 9), Calb1-Cre (Calb1-2A-dgCre-D; Jackson Labs, n = 3), and Thy1-ChR2-YFP (B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J, Jackson Labs; n = 2) lines. We also used 28 wild-type males and females Wistar rats. Animals were maintained in a 12 h light–dark cycle (7 a.m. to 7 p.m.) with access to food and drink ad libitum.
Thy1 chr2 yfp b6 cg tg thy1 cop4 eyfp 18gfng j
Thy1-ChR2-YFP (B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J) is a transgenic mouse line that expresses channelrhodopsin-2 (ChR2) fused to yellow fluorescent protein (YFP) under the control of the Thy1 promoter. This allows for the optical activation of neurons expressing Thy1 in the mouse brain.
2 protocols using thy1 chr2 yfp b6 cg tg thy1 cop4 eyfp 18gfng j
Neuronal activity monitoring in mice and rats
A total of 25 males and females mice were used from wild-type (C57BL/6J, n = 15), PV-Cre (B6;129P2-Pvalbtm1(cre)Arbr/J; Jackson Labs, n = 9), Calb1-Cre (Calb1-2A-dgCre-D; Jackson Labs, n = 3), and Thy1-ChR2-YFP (B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J, Jackson Labs; n = 2) lines. We also used 28 wild-type males and females Wistar rats. Animals were maintained in a 12 h light–dark cycle (7 a.m. to 7 p.m.) with access to food and drink ad libitum.
Optogenetic Manipulation of Mouse Neurons
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