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Thy1 chr2 yfp b6 cg tg thy1 cop4 eyfp 18gfng j

Manufactured by Jackson ImmunoResearch

Thy1-ChR2-YFP (B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J) is a transgenic mouse line that expresses channelrhodopsin-2 (ChR2) fused to yellow fluorescent protein (YFP) under the control of the Thy1 promoter. This allows for the optical activation of neurons expressing Thy1 in the mouse brain.

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2 protocols using thy1 chr2 yfp b6 cg tg thy1 cop4 eyfp 18gfng j

1

Neuronal activity monitoring in mice and rats

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All protocols and procedures were performed according to the Spanish legislation (R.D. 1201/2005 and L.32/2007) and the European Communities Council Directive 2003 (2003/65/CE) for animal research. Experiments were approved by the Ethics Committee of the Instituto Cajal and the Spanish Research Council. A number of recordings in freely moving rats were obtained at the University of Szeged, Hungary, and were approved by the Animal Care Committee of the University of Szeged.
A total of 25 males and females mice were used from wild-type (C57BL/6J, n = 15), PV-Cre (B6;129P2-Pvalbtm1(cre)Arbr/J; Jackson Labs, n = 9), Calb1-Cre (Calb1-2A-dgCre-D; Jackson Labs, n = 3), and Thy1-ChR2-YFP (B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J, Jackson Labs; n = 2) lines. We also used 28 wild-type males and females Wistar rats. Animals were maintained in a 12 h light–dark cycle (7 a.m. to 7 p.m.) with access to food and drink ad libitum.
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2

Optogenetic Manipulation of Mouse Neurons

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All animal use was approved by the Purdue University animal care and use committee. All mice were housed in a 12/12 h light/dark cycle with full access to food and water. The mice used were: C57BL/6 (The Jackson Laboratory), Thy1-ChR2-YFP (B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J, The Jackson Laboratory), Vglut2-ChR2-YFP(C57BL/6-Tg(Slc17a6-COP4*H134R/EYFP)2Oki/J, The Jackson Laboratory). All mice used in experiments were at postnatal days 58–62. A total of 76 mice were used, including 36 male and 40 female mice.
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