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Anti nf κb p65 subunit antibody

Manufactured by Abcam
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Anti-NF-κB-p65 subunit antibody is a lab equipment product that binds to the p65 subunit of the NF-κB transcription factor. This antibody can be used to detect and quantify the p65 subunit in various experimental applications.

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Lab products found in correlation

Anti e cadherin, by R&D Systems (2 mentions) Anti α smooth muscle actin, by R&D Systems (2 mentions) Anti mcp 1, by Abcam (2 mentions)

Close spelling variants of this product

NF-κB p65 (166 citations) NF-κB (121 citations) Anti-NF-κB p65 (89 citations) Anti-NF-κB (71 citations) Anti-p65 (63 citations) Anti-NF-κB p65 antibody (23 citations) Anti-p65 antibody (20 citations) Anti-NF-κB antibody (17 citations) NF-κB p65 antibody (14 citations) NF-κB antibody (12 citations)

2 protocols using anti nf κb p65 subunit antibody

1

Protein Expression Analysis in Cell Types

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell protein and nuclear protein preparation, as well as western blotting, were performed as we described previously [24 (link), 25 (link)]. For whole protein, the membrane was probed with primary antibodies of anti-E-cadherin (rabbit polyclonal, R&D System, 1:1000), anti-α-smooth muscle actin (SMA) (rabbit polyclonal, R&D System, 1:1000), anti-fibroblast specific protein (FSP)-1 (rabbit polyclonal, Abcam, 1:1000), anti-collagen I (rabbit polyclonal, Calbiochem, 1:1000) and anti-MCP-1 (rabbit polyclonal, Abcam, 1:500); for nuclear protein, the membrane was probed with anti-NF-κB-p65 subunit antibody (rabbit polyclonal, Abcam, 1:1000). The intensities of the blots were determined using an imaging analysis program (Image J, free download from http://rsbweb.nih.gov/ij/). The β-actin was used as internal control. The normalized values in different groups were averaged and expressed as fold change with the mean value of control group as 1.
Hu J., Zhu Q., Li P.L., Wang W., Yi F, & Li N. (2015). Stem Cell Conditioned Culture Media Attenuated Albumin-Induced Epithelial– Mesenchymal Transition in Renal Tubular Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 35(5), 1719-1728.
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2

Protein Expression Analysis in Cell Types

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell protein and nuclear protein preparation, as well as western blotting, were performed as we described previously [24 (link), 25 (link)]. For whole protein, the membrane was probed with primary antibodies of anti-E-cadherin (rabbit polyclonal, R&D System, 1:1000), anti-α-smooth muscle actin (SMA) (rabbit polyclonal, R&D System, 1:1000), anti-fibroblast specific protein (FSP)-1 (rabbit polyclonal, Abcam, 1:1000), anti-collagen I (rabbit polyclonal, Calbiochem, 1:1000) and anti-MCP-1 (rabbit polyclonal, Abcam, 1:500); for nuclear protein, the membrane was probed with anti-NF-κB-p65 subunit antibody (rabbit polyclonal, Abcam, 1:1000). The intensities of the blots were determined using an imaging analysis program (Image J, free download from http://rsbweb.nih.gov/ij/). The β-actin was used as internal control. The normalized values in different groups were averaged and expressed as fold change with the mean value of control group as 1.
Hu J., Zhu Q., Li P.L., Wang W., Yi F, & Li N. (2015). Stem Cell Conditioned Culture Media Attenuated Albumin-Induced Epithelial– Mesenchymal Transition in Renal Tubular Cells. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 35(5), 1719-1728.
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