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4 protocols using lactose

1

Synthesis of Fluorescent Nanoparticles

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FeCl3·6H2O, trisodium
citrate dehydrate, ethylene glycol, urea, resorcinol, ammonia, formaldehyde,
silane coupling agent KH550, maleic anhydride, CuCl2 NaOH,
NH2OH·HCl, glucose, K2HPO4,
KH2PO4, NaCl, NH4Cl, MgSO4·7H2O, and CaCl2 were purchased from Sinopharm
Chemical Reagent Co., Ltd. Lactose was purchased from Shanghai Macklin
Biochemical Co., Ltd. All chemicals were analytically pure and used
without further purification. Deionized water was used in all synthesis
procedures.
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2

Graphite-Based Electrochemical Sensor Fabrication

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Natural graphite flakes, sulfuric acid (H2SO4) and hydrogen peroxide (H2O2) were purchased from Aladdin, Reagent Co., Ltd. (Shanghai, China). Potassium chloride (KCl), copper sulfate pentahydrate (CuSO4·5H2O), glucose, uric acid (UA), dopamine hydrochloride (DA), sodium chloride (NaCl), acetaminophenol (AP), L-ascorbic acid (AA), and sodium hydroxide (NaOH) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). mannitol, lactose, and maltose were purchased from Macklin Biochemical Technology Co., Ltd. (Shanghai, China).
The apparatus involves scanning electron microscopy (SEM, LEO1530) (Carl Zeiss Microscopy GmbH, Oberkochen, Germany) for characterizing the microstructures of RTEG-F and Cu/RTEG-F, X-ray Diffraction (XRD, Bruker D8 ADVANCE) (Bruker AXS GmbH, Karlsruhe, Germany) for studying the material composition. Electrochemical deposition, amperometric measurements and cyclic voltammetry (CV) were performed in VSP-300 electrochemical interface (BioLogic China, Beijing, China).
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3

Characterization of Carbon Fiber Materials

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All the chemicals were of analytical grade and used as purchased without further purification. CF (thickness of 1 mm) were supplied by Suzhou Taili New Energy Materials Ltd. Co (P.R. China). Sodium hydroxide, sodium chloride, glucose, dopamine, ascorbic acid, uric acid, cysteine, sucrose, lactose, and maltose were supplied by Macklin Inc, Shanghai, China.
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4

Blueberry Juice Anthocyanin Production

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Concentrated blueberry juice was provided by Kweichow Moutai Ecological Agriculture Industry Development Co., Ltd. (Guizhou, China). It was made from rabbit-eye blueberries from Kaili (Guizhou, China) through pulping, enzymolysis, centrifugation, concentration, and sterilization on an industrial scale. The anthocyanin content in blueberry juice detected by Key Laboratory of Carbohydrate Chemistry and Biotechnology is shown in Table S1 (Zhou et al., 2024 (link)). The cloning host Escherichia coli JM109, expression strain P. pastoris GS115, and expression vector pPIC9K were stored in Key Laboratory of Carbohydrate Chemistry and Biotechnology. The expression strain S. cerevisiae EBY100 and expression vector pYD1 were generously provided by South China Agricultural University. The gene used in this experiment was the PAD from B. amyloliquefaciens (GenBank accession number YP_003921894.1). Anti-DDDDK Tag Mouse Monoclonal Antibody was purchased from Abbkine (California, USA), FITC AffiniPure Goat Anti-Mouse IgG from Earthox (California, USA), Yeast Competent Cell Preps Kit from Sango Biotech (Shanghai, China), lactose from Macklin Biochemical (Shanghai, China), acid hydrolyzed casein from Macklin Biochemical (Shanghai, China), and yeast nitrogen base from Sango Biotech (Shanghai, China).
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