Terminal urodynamic recordings were performed similar to Cheng et al, 200433 (link). Briefly, rats were anaesthetized at 14 w post SCI with 0.8 g/kg urethane delivered subcutaneously. A polyethylene-50 catheter was carefully inserted through the urethra into the bladder for delivery of saline. Fine wire electrodes (0.003” diameter Teflon-insulated silver wire, A-M Systems) were inserted percutaneously via the vagina on both sides of the urethra to monitor the EUS electromyography (EMG) activity. The electrodes were connected to a preamplifier (HZP; Grass-Technologies), which was connected to an amplifier (Grass-Technologies) with low and high-pass frequency filters at 30Hz and 3kHz respectively, and signal was sampled at a rate of 10 kHz (Power 1401, Spike2; Cambridge Electronic Design). Continuous cystometrograms (CMGs) were collected using constant infusion (6 ml/h) of room temperature saline (Aladdin-1000 single syringe infusion pump; World Precision Instruments) through the catheter into the bladder to elicit repetitive voids. The bladder pressure was recorded via the same catheter used for saline infusion, using a pressure transducer (GrassTechnologies) connected to the recording system and sampled at a frequency of 2kHz. Animals that received methysergide did not receive urodynamic analysis. n=11 vehicle, 15 ISP, 6 ILP.
Pressure transducer
Manufactured by Natus
Sourced in United States
A pressure transducer is a device that converts pressure into an electrical signal, allowing the measurement and monitoring of various pressure-related parameters. It functions by detecting changes in pressure and converting them into a corresponding electrical output, which can then be processed and interpreted by other systems or devices.
Automatically generated - may contain errors
5 protocols using pressure transducer
1
Urodynamic Recordings Post Spinal Cord Injury
2
Bladder Pressure and Voiding Evaluation
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Two days before the CMG test, a bladder catheter (PE-50 tubing with a flared tip) was inserted into the PFD rat. The catheter was connected to a syringe pump (KD Scientific, New Hope, PA, USA) and a pressure transducer (Grass Instruments, West Warwick, RI, USA). Each bladder was filled with saline via the catheter at 5 ml/h. A voiding contraction was defined as an increase of bladder pressure that resulted in urine loss as detected by a force transducer (Grass Instruments) that was calibrated to measure volume. Three fills and voids were recorded on each rat. Mean bladder baseline pressure, mean voided volume, mean peak voiding pressure, and mean increase in bladder pressure for voiding (peak voiding pressure minus bladder baseline pressure) were calculated for each animal with a chart recorder.
3
Bladder Function Assessment Protocol
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Two d prior to the conscious CMG test, the bladder catheter (PE-50 tubing with a flitted tip) was inserted into the PFD rats. The catheter was connected to a syringe pump (KD Scientific, New Hope, PA, USA) and a pressure transducer (Grass Instruments, West Warwick, RI, USA). Each bladder was filled with saline solution through the catheter at a speed of 5 mL/h. A voiding contraction was defined as the increase in bladder pressure, which resulted in a loss of urine volume, as detected by a calibrated force transducer (Grass Instruments). Three filling and voidings were recorded in each rat. The mean values of bladder baseline pressure, urinary volume, peak voiding pressure, and urinary pressure increase (peak urination pressure minus the bladder baseline pressure) was calculated for each animal, according to chart recorder results [9 (link)].
4
Urodynamic Recordings Post Spinal Cord Injury
5
Bladder Function Measurement in Rats
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Two days before CMG, a bladder catheter (PE-50 tubing with a flared tip) was inserted into the PFD rat. The catheter was connected to a syringe pump (KD Scientific, New Hope, PA, USA) and a pressure transducer (Grass Instruments, West Warwick, RI, USA). Each bladder was filled with saline via the catheter at 5 ml/hour. A voiding contraction was defined as an increase of bladder pressure that resulted in urine loss as detected by a force transducer (Grass Instruments) that was calibrated to measure volume. Three fills and voids were recorded for each rat. Mean bladder baseline pressure, mean voided volume, mean peak voiding pressure, and mean increase in bladder pressure for voiding (peak voiding pressure minus bladder baseline pressure) were calculated for each animal with a chart recorder.
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