Hepatic cell lines HepG2, Huh-7, QSG-7701, and L-02 were maintained in complete DMEM (Invitrogen, Carlsbad, CA, USA). Cells were treated with 100 IU/mL of IFN-α2b (Sangon, Shanghai, China) for 2 days and collected for analysis. The HepAD38 cell line (kindly provided by Prof. Christoph Seeger from Fox Chase Cancer Center, Philadelphia), which supports HBV replication under tetracycline control, was routinely maintained in complete DMEM supplemented with 380 μg/mL G418 antibiotic and 1 μg/mL tetracycline (Sigma, St. Louis, MO, USA). To support the stable production of HBV, tetracycline was removed from the culture medium at least 2 weeks prior.
Ifn α2b
Manufactured by Sangon
Sourced in China
IFN-α2b is a recombinant human interferon alpha-2b protein. It is used as a research tool in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
G418 antibiotic, by Merck Group (1 mentions)
Complete dmem, by Thermo Fisher Scientific (1 mentions)
Tetracycline, by Merck Group (1 mentions)
Complete dmem, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Ribavirin, by Merck Group (1 mentions)
Marc 145 cells, by American Type Culture Collection (1 mentions)
Bhk 21 cells, by American Type Culture Collection (1 mentions)
Chloroquine, by MedChemExpress (1 mentions)
5 fluorouracil, by MedChemExpress (1 mentions)
2 protocols using ifn α2b
1
HBV Replication Regulation in Hepatic Cells
2
Cultivating Cell Lines and Characterizing PRRSV
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MARC-145 cells (ATCC) were cultured in Modified Eagle Medium (MEM; Sigma-Aldrich, St. Louis, MO, United States) containing 10% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, United States) and 1% penicillin–streptomycin (Thermo Fisher Scientific, Waltham, MA, United States). BHK-21 cells purchased from ATCC were maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% FBS (Gemini Bio, West Sacramento, CA, United States) and 1% penicillin–streptomycin (Thermo Fisher Scientific, Waltham, MA, United States). The HP-PRRSV TA-12 strain (GenBank Accession No. HQ416720.1) was purified by plaque assay in MARC-145 cells, and the complete genome of one plaque was determined by the Sanger sequencing method and deposited to GenBank under accession No. MZ399801. A monoclonal antibody (clone 4A5) against PRRSV N protein was purchased from MEDIAN Diagnostics, Korea. IFN-α2b (Sangon Biotech, Shanghai, China), ribavirin (Sigma-Aldrich, St. Louis, MO, United States), 5-Fluorouracil (MedChemExpress, Shanghai, China), and Chloroquine (MedChemExpress, Shanghai, China) were used in this study. Coelenterazine h (Maokang Biotechnology, Shanghai, China) was dissolved in acidified methanol to a concentration of 5 mg/ml, and aliquots were stored at −80°C.
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