Anti phospho t172 ampkα

Anti-LC3B (ab51520), anti-S6K1 (ab32359), anti-phospho-T389 S6K1 (ab2571) and horseradish peroxidase (HRP) -labelled anti-horse IgG secondary antibodies (ab6802) were purchased from Abcam. Anti-GST (sc-459) was purchased from Santa Cruz Biotechnology. Anti-p62 (CST 5114), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (CST 2118), anti-phospho-S65 4E-BP1 (CST 9451), anti-phospho-S757 ULK1 (CST 6888), anti-phospho-S473 Akt (CST 4058), anti-phospho-T172 AMPKα (CST 2531) and HRP-labelled anti-rabbit IgG secondary antibodies (CST 7074) were purchased from Cell Signaling Technology. His-tagged LRS and GST-tagged RagD were in laboratory stocks. mCherry-GFP-LC3 plasmid (pBabe vector) was from Dr Heesun Cheong, Division of Chemical Biology, Research Institute, National Cancer Center, Korea.

Cells were lysed in RIPA-SDS buffer, after which the proteins were resolved by
SDS-PAGE, transferred onto PVDF membranes, and probed with the relevant
antibodies as previously described9 (link). The following antibodies
were used for the experiments: anti-actin (Sigma); anti-SESN2 (Proteintech),
anti-NRF2, anti-CHOP and anti-p53 (Santa Cruz); and anti-phospho(T389)-p70S6K,
anti-phospho(S235/236)-S6, anti-phospho(S65)-4E-BP1, anti-phospho
IF2α(S51), anti-phospho(T172)-AMPKα,
anti-phospho(S79)-acetyl-CoA carboxylase, anti-phospho(S9)-GSK3β,
anti-phospho(T1462), anti-4E-BP1, anti-S6, anti-TSC2, anti-AMPKα,
anti-ATF4, anti-Bip, anti-p62, anti-LC3, anti-PARP, anti-PARP(cleaved),
anti-caspase3, and anti-caspase3(cleaved) (Cell Signaling Inc). Sesn1 antibodies
were previously described11 (link).