NIH3T3 cells were purchased from American Type Culture Collection (ATCC). Ba/F3, WEHI, and BOSC23 cells were kindly provided by Dr. Daniel G. Tenen (Harvard Medical School). Crizotinib, ceritinib, alectinib, brigatinib, lorlatinib, entrectinib, gilteritinib, and repotrectinib were purchased from Selleck. NIH3T3 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 100 mg/ml streptomycin (P/S). Parental Ba/F3 cells were maintained in RPMI1640 supplemented with 5% WEHI (as a source of IL-3), 10% FBS, and P/S. Ba/F3 cells expressing CLIP1-LTK mutants were maintained in RPMI1640 supplemented with 10% FBS and P/S. All cell lines were routinely tested for mycoplasma infection and negative for mycoplasma infection.
Repotrectinib
Manufactured by Selleck Chemicals
Repotrectinib is a laboratory equipment product used for scientific research purposes. It is a multi-targeted tyrosine kinase inhibitor. The core function of Repotrectinib is to inhibit the activity of certain enzymes involved in cellular signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Entrectinib, by Selleck Chemicals (2 mentions)
Nih3t3 cells, by American Type Culture Collection (1 mentions)
Alectinib, by Selleck Chemicals (1 mentions)
Lorlatinib, by Selleck Chemicals (1 mentions)
Brigatinib, by Selleck Chemicals (1 mentions)
Gilteritinib, by Selleck Chemicals (1 mentions)
Crizotinib, by Selleck Chemicals (1 mentions)
Ceritinib, by Selleck Chemicals (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Gefitinib, by Selleck Chemicals (1 mentions)
Trametinib, by Selleck Chemicals (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
Gdc 0941, by Selleck Chemicals (1 mentions)
Pc9 del e746 a750, by RIKEN Cell Bank (1 mentions)
2 protocols using repotrectinib
1
Cell Line Maintenance and Compound Screening
2
Characterization of metastatic colon cancer cell line KM12SM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human colon cancer cell line KM12SM was kindly gifted by Dr. I. J. Fidler (MD Anderson Cancer Center) to our group in 1999. KM12SM cells are a highly metastatic variant of KM12C cells, which present the TPM3‐NTRK1 gene rearrangement.8 , 9 KM12SM cells were maintained and cultured in RPMI‐1640 medium (Thermo Fischer Scientific) with 10% FBS, penicillin (100 units/ml), and streptomycin (50 μg/ml), in a humidified CO2 incubator at 37°C. Ba/F3 transfected with wild‐type TPM3‐NTRK1 (Ba/F3_WT) and Ba/F3 transfected with TPM3‐NTRK1 G595R (Ba/F3_G595R) cells were gifted by Dr. R. Katayama (Japanese Foundation for Cancer Research). Ba/F3_WT, Ba/F3_G595R cells were cultured in DMEM containing 10% FBS. The EGFR‐mutant human lung adenocarcinoma cell line PC9 (del E746_A750) was purchased from RIKEN Cell Bank. Cells were regularly screened for mycoplasma using a MycoAlert mycoplasma detection kit (Lonza). Cell‐line authentication was performed by a short tandem repeat analysis in a laboratory at the National Institute of Biomedical Innovation (Osaka, Japan) in May 2015. Entrectinib, repotrectinib, GDC‐0941, trametinib and gefitinib were purchased from Selleck Chemicals.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!