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2 oxoadipic acid

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2-oxoadipic acid is a chemical compound that is used as a laboratory reagent. It is a dicarboxylic acid with the molecular formula C6H8O4. The primary function of 2-oxoadipic acid is as a chemical intermediate in various organic synthesis reactions.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Merck Group (2 mentions) Quinolinic acid, by Merck Group (2 mentions) Glycerol, by Thermo Fisher Scientific (1 mentions) Micrococcal nuclease, by Thermo Fisher Scientific (1 mentions) 1 1 carbonyldiimidazole cdi, by Merck Group (1 mentions) 2 oxoglutaric acid, by Merck Group (1 mentions) Ni sepharose high performance affinity media, by GE Healthcare (1 mentions) Benzamidine hcl, by Merck Group (1 mentions) Dnase 1, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Iodoacetamide, by Merck Group (1 mentions) Glutaric acid, by Merck Group (1 mentions) Riboflavin, by Merck Group (1 mentions) Xanthurenic acid, by Merck Group (1 mentions) Chlorpropamide, by Merck Group (1 mentions) Pantothenic acid, by Merck Group (1 mentions) Debrisoquine sulfate, by Merck Group (1 mentions) Taurine, by Merck Group (1 mentions) Uric acid, by Merck Group (1 mentions) D ribose, by Merck Group (1 mentions)

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Oxoadipic acid (2 citations)

4 protocols using 2 oxoadipic acid

1

Affinity-based Protein Purification Methods

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ThDP, NAD+, CoA, DTT, isopropyl 1-thio-β-D-galactopyranoside, imidazole, thiamin-HCl, glycerol, DNase I, and Micrococcal nuclease were from Affymetrix (Thermo Fisher Scientific, Waltham, MA, USA); 2-oxoglutaric acid, 2-oxoadipic acid, iodoacetamide, benzamidine HCl, disuccinimidyl dibutyric urea (DSBU or BuUrBu), 1.1′-carbonyl-diimidazole (CDI), and dimethyl sulfoxide were from Sigma-Aldrich (St. Louis, MO, USA). Formic acid solution was obtained from Fluka Analytical (Buchs, Switzerland); MS grade trypsin protease was from Pierce; Ni Sepharose® high-performance affinity media was from GE Healthcare (Chicago, IL, USA).
Ozohanics O., Zhang X., Nemeria N.S., Ambrus A, & Jordan F. (2023). Probing the E1o-E2o and E1a-E2o Interactions in Binary Subcomplexes of the Human 2-Oxoglutarate Dehydrogenase and 2-Oxoadipate Dehydrogenase Complexes by Chemical Cross-Linking Mass Spectrometry and Molecular Dynamics Simulation. International Journal of Molecular Sciences, 24(5), 4555.
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2

Metabolite Profiling of Biological Samples

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All chemicals and solvents utilized were of the highest purity available. Water and acetonitrile were Optima LC/MS grade (Fisher Scientific Inc). Debrisoquine sulfate, 4-nitrobenzoic acid, and chlorpropamide were utilized as internal standards (Sigma-Aldrich®,St. Louis, MO). Riboflavin, kynurenic acid, pantothenic acid, uric acid, 2-oxoadipic acid, D-ribose, D-glucose, hippuric acid, glutaric acid, xanthurenic acid, and taurine were also purchased from Sigma-Aldrich® (St. Louis, MO). 5′-deoxy-5′-methylthioadenosine was purchased from Santa Cruz Biotechnology Inc (Dallas, TX).
Laiakis E.C., Mak T.D., Strawn S.J., Wang Y.W., Moon B.H., Ake P, & Fornace AJ J.r. (2018). Global metabolomic responses in urine from Atm deficient mice in response to LD50/30 gamma irradiation doses. Environmental and molecular mutagenesis, 59(7), 576-585.
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3

Mitochondrial Respiratory Chain Analysis

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Age-matched control and patient fibroblasts from the Newcastle Biobank were grown in high glucose Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Poole, UK) supplemented with 10% FBS and 1% penicillin/streptomycin. SH-SY5Y cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, 2 mM glutamine, and 1% penicillin/streptomycin. Blue native polyacrylamide gel electrophoresis (BN-PAGE) of the mitochondrial respiratory chain complexes was performed as described previously14 (link). For drug toxicity studies growth medium was supplemented with 5 and 50 µM 2-oxoadipic acid (Sigma 75447) and quinolinic acid (Sigma) respectively for 4 days before analysis. Experiments were done in triplicates.
Boczonadi V., King M.S., Smith A.C., Olahova M., Bansagi B., Roos A., Eyassu F., Borchers C., Ramesh V., Lochmüller H., Polvikoski T., Whittaker R.G., Pyle A., Griffin H., Taylor R.W., Chinnery P.F., Robinson A.J., Kunji E.R, & Horvath R. (2018). Mitochondrial oxodicarboxylate carrier deficiency is associated with mitochondrial DNA depletion and spinal muscular atrophy-like disease. Genetics in medicine : official journal of the American College of Medical Genetics, 20(10), 1224-1235.
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4

Mitochondrial Respiratory Chain Analysis

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Age-matched control and patient fibroblasts from the Newcastle Biobank were grown in high glucose Dulbecco’s modified Eagle’s medium (DMEM, Sigma, Poole, UK) supplemented with 10% FBS and 1% penicillin/streptomycin. SH-SY5Y cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum, 2 mM glutamine, and 1% penicillin/streptomycin. Blue native polyacrylamide gel electrophoresis (BN-PAGE) of the mitochondrial respiratory chain complexes was performed as described previously14 (link). For drug toxicity studies growth medium was supplemented with 5 and 50 µM 2-oxoadipic acid (Sigma 75447) and quinolinic acid (Sigma) respectively for 4 days before analysis. Experiments were done in triplicates.
Boczonadi V., King M.S., Smith A.C., Olahova M., Bansagi B., Roos A., Eyassu F., Borchers C., Ramesh V., Lochmüller H., Polvikoski T., Whittaker R.G., Pyle A., Griffin H., Taylor R.W., Chinnery P.F., Robinson A.J., Kunji E.R, & Horvath R. (2018). Mitochondrial oxodicarboxylate carrier deficiency is associated with mitochondrial DNA depletion and spinal muscular atrophy-like disease. Genetics in medicine : official journal of the American College of Medical Genetics, 20(10), 1224-1235.
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