4 μL of 0.1 mg/mL ADDoCoV protein sample dialyzed into 25 mM HEPES pH 7.5, 150 mM NaCl, 2 mM EDTA was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu grid (Electron Microscopy Sciences), incubated for 1 min, and manually blotted. 4 μL of 3% Uranyl Acetate was applied onto the same grid and incubated for 1 min before the solution was blotted off. Images were acquired at a nominal magnification of 49 000× on a FEI Tecnai 12 120 kV BioTwin Spirit microscope equipped with an Eagle 4 k × 4 k CCD camera.
Tecnai 12 120 kv biotwin spirit microscope
Manufactured by Thermo Fisher Scientific
The Tecnai 12 120 kV BioTwin Spirit microscope is a transmission electron microscope (TEM) designed for biological sample analysis. It operates at an accelerating voltage of 120 kV and features the BioTwin optical configuration. The microscope is capable of high-resolution imaging and analysis of biological specimens.
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Lab products found in correlation
2 protocols using tecnai 12 120 kv biotwin spirit microscope
1
Negative Stain Imaging of ADDoCoV
2
SARS-CoV-2 Spike Protein Visualization
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Four microliters of SARS-CoV S protein (0.05 mg/ml) was applied onto a freshly glow discharged (1 min at 10 mA) CF300-Cu-50 grid (Electron Microscopy Sciences), incubated for 1 min, and manually blotted. Four microliters of 3% uranyl acetate was applied onto the same grid and incubated for 1 min before the solution was blotted off. Images were acquired at a nominal magnification of ×49,000 on a FEI Tecnai 12 120-kV BioTwin Spirit microscope.
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