Human sicam 1 elisa kits

Manufactured by Thermo Fisher Scientific

The Human sICAM‐1 ELISA kit is a quantitative sandwich enzyme immunoassay designed for the measurement of human soluble intercellular adhesion molecule 1 (sICAM‐1) in serum, plasma, and cell culture supernatants.

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Lab products found in correlation

Non specific mouse igg, by Jackson ImmunoResearch (2 mentions) Monoclonal mouse anti human icam 1 clone r6.5, by American Type Culture Collection (2 mentions) Phycoerythrin labeled monoclonal mouse anti human icam 1 clone lb 2, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 350 labeled goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) Green fluoresbrite polystyrene particles, by Polysciences (2 mentions) Porous transwell inserts, by Thermo Fisher Scientific (2 mentions) 125iodine 125i, by PerkinElmer (2 mentions) Iodogen pre coated tubes, by PerkinElmer (2 mentions) Mmp 9 inhibitor 1, by Merck Group (2 mentions) Mmp 2 inhibitor 1, by Merck Group (2 mentions)

2 protocols using human sicam 1 elisa kits

Quantifying Leukocyte-Endothelial Interactions

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Monoclonal mouse anti‐human ICAM‐1 (clone R6.5) was from ATCC (Manassas, VA) and phycoerythrin‐labeled monoclonal mouse anti‐human ICAM‐1 (clone LB‐2) was from Santa Cruz Biotechnology (Dallas, TX). Alexa Fluor 350‐labeled goat anti‐mouse IgG was from Invitrogen (Carlsbad, CA). Nonspecific mouse IgG was from Jackson ImmunoResearch (West Grove, PA). Green Fluoresbrite® polystyrene particles (100 nm in diameter) were from Polysciences (Warrington, PA). Porous transwell inserts (1.0 µm‐pore size) were from Thermo Fisher Scientific (Waltham, MA). Human sICAM‐1 ELISA kits were from Invitrogen (Carlsbad, CA). ¹²⁵Iodine (¹²⁵I) and Iodogen pre‐coated tubes were from PerkinElmer (Waltham, MA) and Thermo Fisher Scientific (Waltham, MA), respectively. MMP‐9 Inhibitor I and MMP‐2 Inhibitor I were from EMD Millipore (Billerica, MA). Unless specified, all other reagents were from Sigma‐Aldrich (St. Louis, MO).

Manthe R.L, & Muro S. (2017). ICAM‐1‐targeted nanocarriers attenuate endothelial release of soluble ICAM‐1, an inflammatory regulator. Bioengineering & Translational Medicine, 2(1), 109-119.

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ICAM-1 Immunolabeling Protocol

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Monoclonal mouse anti-human ICAM-1 (clone R6.5) was from ATCC (Manassas, VA) and phycoerythrin-labeled monoclonal mouse anti-human ICAM-1 (clone LB-2) was from Santa Cruz Biotechnology (Dallas, TX). Alexa Fluor 350-labeled goat anti-mouse IgG was from Invitrogen (Carlsbad, CA). Non-specific mouse IgG was from Jackson ImmunoResearch (West Grove, PA). Green Fluoresbrite® polystyrene particles (100 nm in diameter) were from Polysciences (Warrington, PA). Porous transwell inserts (1.0 µm-pore size) were from Thermo Fisher Scientific (Waltham, MA). Human sICAM-1 ELISA kits were from Invitrogen (Carlsbad, CA). ¹²⁵Iodine (¹²⁵I) and Iodogen pre-coated tubes were from PerkinElmer (Waltham, MA) and Thermo Fisher Scientific (Waltham, MA), respectively. MMP-9 Inhibitor I and MMP-2 Inhibitor I were from EMD Millipore (Billerica, MA). Unless specified, all other reagents were from Sigma-Aldrich (St. Louis, MO).

Manthe R.L, & Muro S. (2017). ICAM‐1‐targeted nanocarriers attenuate endothelial release of soluble ICAM‐1, an inflammatory regulator. Bioengineering & Translational Medicine, 2(1), 109-119.

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