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Anti cd34 percp efluor 710

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The Anti-CD34-PerCP-Efluor 710 is a fluorochrome-conjugated monoclonal antibody that binds to the CD34 antigen, which is expressed on hematopoietic stem and progenitor cells. This product is intended for use in flow cytometry applications.

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Lab products found in correlation

Anti cd19 pe, by Thermo Fisher Scientific (2 mentions) Anti cd33 pe cy5, by Beckman Coulter (2 mentions) Anti cd135 biotin, by BD (2 mentions) Anti cd10 alexa 700, by BD (2 mentions) Anti cd7 pacific blue, by BD (2 mentions) Anti cd34 apc cy7, by BD (2 mentions) Anti cd56 alexafluor 647, by Thermo Fisher Scientific (2 mentions) Streptavidin qd605, by Thermo Fisher Scientific (2 mentions) Anti cd3 fitc, by Thermo Fisher Scientific (2 mentions) Anti cd45 v500, by BD (2 mentions) Anti cd38 pe cy7, by BD (2 mentions) Anti cd45ra fitc, by BD (2 mentions) Cd34 microbead kit, by Miltenyi Biotec (2 mentions) Anti cd90 apc, by BD (2 mentions) Facs aria 3 system, by BD (1 mentions)

Spelling variants of this product

PerCP-eFluor 710 (33 citations) Anti-CD34 (23 citations) CD34-PerCP (3 citations)

3 protocols using anti cd34 percp efluor 710

1

Cord Blood Mononuclear Cell Phenotyping

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Mononuclear cells (1 × 106 per 100 μl) from cord blood were stained with the following antibodies (all from BD Biosciences, unless stated otherwise; the dilution used and catalogue numbers are in parentheses): anti-CD45RA–FITC (1:25; 555488), anti-CD90–APC (1:50; 561971), anti-CD135–Biotin (1:10; 624008), anti-CD38–PE-Cy7 (1:200; 335790), anti-CD10–Alexa Fluor 700 (1:10; 624040), anti-CD7–Pacific Blue (1:50; 642916), anti-CD45–V500 (1:200; 560777), anti-CD34–APC-Cy7 (1:100), anti-CD34–PerCP-Efluor 710 (1:100; eBioscience, 46-0344-42), anti-CD33–PE-Cy5 (1:100; Beckman Coulter, PNIM2647U), anti-CD19–PE (1:200), anti-CD3–FITC (1:100; 349201), anti-CD56–Alexa Fluor 647 (1:100; 557711) and Streptavidin–QD605 (1:200; Invitrogen, Q10101MP). Cells were sorted on a FACS Aria III system (BD Bioscience).
Thomas G.E., Egan G., García-Prat L., Botham A., Voisin V., Patel P.S., Hoff F.W., Chin J., Nachmias B., Kaufmann K.B., Khan D.H., Hurren R., Wang X., Gronda M., MacLean N., O’Brien C., Singh R.P., Jones C.L., Harding S.M., Raught B., Arruda A., Minden M.D., Bader G.D., Hakem R., Kornblau S., Dick J.E, & Schimmer A.D. (2022). The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness. Nature Cell Biology, 24(6), 872-884.
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2

AML Immune Profiling Protocol

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Mononuclear cells (1×106/100 μl) from peripheral blood or bone marrow of AML patients were stained with the following antibodies (all from BD unless stated otherwise, dilution used and catalogue number in parentheses): anti-CD45RA-FITC (1:25, 555488), anti-CD90-APC (1:50, 561971), anti-CD135-Biotin (1:10, 624008), anti-CD38-PE-Cy7 (1:200, 335790), anti-CD10-Alexa-700 (1:10, 624040), anti-CD7-Pacific Blue (1:50, 642916), anti-CD45-V500 (1:200, 560777), anti-CD34-APC-Cy7 (1:100, custom made by BD, CD34 clone 581), anti-CD34-PerCP-Efluor 710 (1:100, e-Bioscience 46-0344-42), anti-CD33-PE-Cy5 (1:100, Beckman Coulter PNIM2647U), anti-CD19-PE (1:200, 349204), anti-CD3-FITC (1:100, 349201), anti-CD56-Alexafluor 647 (1:100, 557711), and Streptavidin-QD605 (1:200, Invitrogen Q10101MP). Samples from Patients #1, 10, 11 (remission sample only), 32, 35, and 55 were enriched for CD34+ cells using a Miltenyi CD34 MicroBead kit according to the manufacturer’s protocol prior to antibody staining. Cells were sorted on a FACS AriaIII to a post-sort purity of >95%.
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
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3

AML Immune Profiling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mononuclear cells (1×106/100 μl) from peripheral blood or bone marrow of AML patients were stained with the following antibodies (all from BD unless stated otherwise, dilution used and catalogue number in parentheses): anti-CD45RA-FITC (1:25, 555488), anti-CD90-APC (1:50, 561971), anti-CD135-Biotin (1:10, 624008), anti-CD38-PE-Cy7 (1:200, 335790), anti-CD10-Alexa-700 (1:10, 624040), anti-CD7-Pacific Blue (1:50, 642916), anti-CD45-V500 (1:200, 560777), anti-CD34-APC-Cy7 (1:100, custom made by BD, CD34 clone 581), anti-CD34-PerCP-Efluor 710 (1:100, e-Bioscience 46-0344-42), anti-CD33-PE-Cy5 (1:100, Beckman Coulter PNIM2647U), anti-CD19-PE (1:200, 349204), anti-CD3-FITC (1:100, 349201), anti-CD56-Alexafluor 647 (1:100, 557711), and Streptavidin-QD605 (1:200, Invitrogen Q10101MP). Samples from Patients #1, 10, 11 (remission sample only), 32, 35, and 55 were enriched for CD34+ cells using a Miltenyi CD34 MicroBead kit according to the manufacturer’s protocol prior to antibody staining. Cells were sorted on a FACS AriaIII to a post-sort purity of >95%.
Shlush L.I., Zandi S., Mitchell A., Chen W.C., Brandwein J.M., Gupta V., Kennedy J.A., Schimmer A.D., Schuh A.C., Yee K.W., McLeod J.L., Doedens M., Medeiros J.J., Marke R., Kim H.J., Lee K., McPherson J.D., Hudson T.J., Brown A.M., Trinh Q.M., Stein L.D., Minden M.D., Wang J.C, & Dick J.E. (2014). Identification of pre-leukemic hematopoietic stem cells in acute leukemia. Nature, 506(7488), 328-333.
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