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Anti dna rna damage

Manufactured by Abcam
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The Anti-DNA/RNA damage product is a lab equipment designed to detect and quantify DNA and RNA damage in biological samples. It provides a reliable and sensitive method for measuring various types of DNA and RNA lesions without interpretation or extrapolation on intended use.

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Lab products found in correlation

Summit 4, by Beckman Coulter (1 mentions) Cyan adp instrument, by Beckman Coulter (1 mentions) Anti cd31, by BioLegend (1 mentions) Zombie dye, by BioLegend (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) F4 80, by Bio-Rad (1 mentions) Sca1 apc, by Miltenyi Biotec (1 mentions) Keratin 14, by BioLegend (1 mentions) Itga6, by BD (1 mentions) Cd34 fitc, by Thermo Fisher Scientific (1 mentions) Nf κb, by Santa Cruz Biotechnology (1 mentions) Col17a1, by Abcam (1 mentions) Tom20, by Thermo Fisher Scientific (1 mentions) Prb 435p, by Thermo Fisher Scientific (1 mentions) Cellsens standard v1, by Olympus (1 mentions) Keratin 1, by Abcam (1 mentions) Casp3, by Cell Signaling Technology (1 mentions) Cd45 apc cy7, by BioLegend (1 mentions) Cd11b, by BD (1 mentions)

2 protocols using anti dna rna damage

1

Quantifying Endothelial and Cardiomyocyte Stress

Cited 1 time
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Flow cytometric analysis of endothelial and cardiomyocyte oxidative stress and IL-36R was performed as previously described (15 (link)). Briefly, hearts were collagenase-digested to obtain single-cell suspensions. Cells were incubated with antibodies: anti-DNA/RNA damage (1:100 dilution, Abcam), anti-IL-36R (1:100 dilution, R & D Systems; Alexa-647 secondary, 1:100 dilution, Biolegend), anti-CD31 (1:100 dilution, Biolegend), anti-cTnT (1:100 dilution, Miltenyi Biotec), and Zombie dye (1:500 dilution, Biolegend), along with appropriate IgG controls. Flow cytometry using a CyAn™ ADP instrument (Beckman Coulter, USA) captured 250,000 events per sample. Data analysis was performed with Summit 4.3 software (Beckman Coulter, USA).
El-Awaisi J., Mitchell J.L., Ranasinghe A, & Kalia N. (2023). Interleukin-36 is vasculoprotective in both sexes despite sex-specific changes in the coronary microcirculation response to IR injury. Frontiers in Cardiovascular Medicine, 10, 1227499.
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2

Multicolor Immunofluorescence Staining Protocol

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Immunofluorescence was performed as described previously2 (link). The following antibodies or chemical compounds were used: COL17A1 (Abcam, ab186415, 1:400), Keratin 14 (Biolegend, 906004, 1:500), ITGA6 (BD, 555734, 1:200), Keratin 15 (Biolegend, 833904, 1:300), NFκB (Santa Cruz, sc-101749, 1:50), CD11b (BD, 557686, 1:100), F4/80 (Bio-Rad, MCA497A488, 1:100), KI67 (Invitrogen, 14-5698-82, 1:300), CD3 (Biolegend, 100235, 1:100), MHC2 (Biolegend, 107605, 1:100), CD31(BD Biosciences, 557355, 1:200), anti-DNA/RNA Damage (Abcam, ab62623, 1:2000), Tom20 (Thermo Fisher, 11802-1-AP, 1:30), Keratin 1 (Abcam, ab185628, 1:300), survivin (Cell signaling, 2808, 1:100), casp3 (Cell signaling, 9661, 1:200), Tuj1 (Covance, PRB-435P, 1:500), CD34-FITC (Invitrogen, 11-0341-85, 1:100), ITGA6-PE (BD, 555736, 1:100), Sca1-APC (Miltenyi Biotec, 130-102-343, 1:100), CD45-APC-cy7 (BioLegend, 103116, 1:100). FV10-ASW4.2 was used for capturing confocal images. CellSens Standard v1.13 (Olympus) was used for collecting HE data.
Morinaga H., Mohri Y., Grachtchouk M., Asakawa K., Matsumura H., Oshima M., Takayama N., Kato T., Nishimori Y., Sorimachi Y., Takubo K., Suganami T., Iwama A., Iwakura Y., Dlugosz A.A, & Nishimura E.K. (2021). Obesity accelerates hair thinning by stem cell-centric converging mechanism. Nature, 595(7866), 266-271.
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