Antiatg7 antibody

Total protein was extracted from spermatogenic cells of testicular tissue and NTERA-2 cells. Western blotting for ATG7, LC3, and Beclin-1 was performed as described in our previous work ⁸. The membrane was incubated with antiATG7 antibody (1:1000; Abcam, Cambridge, UK), antiLC3 antibody (1:1000; Abcam), antiBeclin-1 antibody (1:1000; Abcam) and antiβ-actin antibody (1:1000; Abcam) overnight at 4 ºC and incubated with secondary antibodies (1:1000; Sanying Biotechnology, Wuhan, China) for 2 h at 23 °C.

The SH-SY5Y cell line of human neuroblastoma was collected from the Stem Cell Bank of the Chinese Academy of Sciences. Semaglutide (peptide purity: 95.77%) and liraglutide (peptide purity: 95.77%) were obtained from Synpeptide Co. (Shanghai, China); 6-OHDA and the anti-LC3-II antibody were obtained from Sigma-Aldrich (St. Louis, MO, USA); the anti-P62 antibody, anti- Atg7 antibody, and anti-beclin1 antibody were obtained from Abcam (Cambridge, UK); and the β-actin antibody was obtained from Bioworld Technology Co. (Shanghai, China). The reactive oxygen species (ROS) assay kit and mitochondrial membrane potential assay kit with JC-1 were purchased from Nanjing KeyGen Biotechnology Company (Nanjing, China).