Av 400

Manufactured by Bruker

Sourced in Germany, United States, Switzerland, United Kingdom, China, Japan

The AV-400 is a nuclear magnetic resonance (NMR) spectrometer designed for analytical purposes. It provides a magnetic field of 9.4 Tesla, operating at a proton frequency of 400 MHz. The AV-400 is capable of performing standard NMR experiments to analyze the structural and chemical properties of samples.

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Lab products found in correlation

Av 500, by Bruker (30 mentions) Av 300, by Bruker (27 mentions) Av 600, by Bruker (13 mentions) Drx 500, by Bruker (12 mentions) Silica gel, by Qingdao Marine Chemical (7 mentions) Dpx 400, by Bruker (6 mentions) Av 600 spectrometer, by Bruker (6 mentions) Kieselgel 60, by Merck Group (5 mentions) P 1020, by Jasco (5 mentions) Sephadex lh 20, by Cytiva (5 mentions) 2400 series 2 chns o analyzer, by PerkinElmer (5 mentions) Q tof ultima, by Waters Corporation (4 mentions) Amx 500, by JEOL (4 mentions) Lcq xp, by Thermo Fisher Scientific (4 mentions) U 3000, by Hitachi (4 mentions) Dpx 300, by Bruker (4 mentions) Vertex 70, by Bruker (4 mentions) Silica gel 60, by Merck Group (4 mentions) Lcms it tof, by Shimadzu (4 mentions) Av 500 spectrometer, by Bruker (3 mentions)

Close spelling variants of this product

AV 400 MHz NMR spectrometer (10 citations) AV III-400 NMR spectrometer (5 citations) AV II-400 MHz spectrometer (8 citations) AV 400 MHz spectrometer (51 citations) AV‐III 400 MHz instrument (2 citations) AV II-400 spectrometer (7 citations) AV-400 instrument (18 citations) AVANCE AV III 400 (8 citations) Advance AV-400 (2 citations) AV-400 NMR spectrometer (53 citations)

279 protocols using av 400

Synthesis and Characterization of Heterocyclic Compounds

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The reagents were purchased from commercial sources and were used as received. All anhydrous solvents were dried and purified by standard techniques just before use. Matrine was purchased from Baoji Biological Development Co., Ltd. (Xi’an, China). Compound A [28 (link)], C [46 (link)] and D [46 (link)] were synthesized as referred by the literature.
The reaction progress was monitored by thin-layer chromatography on silica gel GF254 with detection by UV. Microwave reaction was conducted in a microwave synthesizer (CEM Discover SP). Melting points were determined using an X-4 binocular microscope melting point apparatus and the thermometer was uncorrected. The ¹H NMR spectra were obtained by using a Bruker AV 400 with CDCl₃ or DMSO-d₆ as a solvent. Chemical shifts (δ) were given in parts per million (ppm) and were measured downfield from internal tetramethylsilane. The ¹³C NMR spectra were recorded by using a Bruker AV 400 (100 MHz) with CDCl₃ or DMSO-d₆ as a solvent. Chemical shifts (δ) were reported in parts per million using the solvent peak as the standard. High-resolution mass spectra were obtained with an FT-ICR MS spectrometer (Ionspec, 7.0 T).

Ni W., Song H., Wang L., Liu Y, & Wang Q. (2023). Design, Synthesis and Various Bioactivity of Acylhydrazone-Containing Matrine Analogues. Molecules, 28(10), 4163.

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Isolation and Characterization of Bulleyaconitine A

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Bulleyaconitine A was
purchased from Wuhan Yuancheng Technology Development Co., Ltd. Unless
otherwise specified, the reagents and solvents used in this article
are all commercially available analytical or chemical grades and used
directly without any purification. The high-resolution mass spectrometry
(HRMS) spectrum was determined by a Waters ACQUITY UPLC/Xevo G2-S
QTOF mass spectrometer. ¹H NMR spectra were recorded on
a Bruker AV 400 nuclear magnetic resonance instrument (400 MHz). Chemical
shifts were recorded in parts per million (ppm) relative to tetramethylsilane
as the internal standard. Data were reported as follows: chemical
shift, multiplicity (s, singlet; d, doublet; t, triplet; q, quartet;
dd, doublet–doublet; dt, doublet–triplet; m, multiplet;
br, broad), coupling constants (Hz), integration. ¹³C NMR
data were collected on a Bruker AV 400 nuclear magnetic resonance
instrument (100 MHz) with complete proton decoupling. Chemical shifts
were reported in ppm with the tetramethylsilane as the internal standard.
Thin-layer chromatography silica gel GF254 and column chromatography
silica gel G and H (200–400 mesh) were produced by Qingdao
Ocean Chemical Plant.

Zhang X., Shang Y.S., Gao F., Fang D.M., Li X.H, & Zhou X.L. (2020). Synthesis and Evaluation of a Series of New Bulleyaconitine A Derivatives as Analgesics. ACS Omega, 5(33), 21211-21218.

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NMR Analysis of Larval Hemolymph Metabolites

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Hemolymph from 20 larvae of P. cochleariae or C. populi was collected and taken up in 200 μl CD₃OD for ¹H/²D-exchange. The solution was concentrated under reduced pressure and dissolved in 500 μl CD₃OD. One-dimensional ¹H NMR spectra were recorded on a Bruker AV400 using water suppression (purge). Two-dimensional double quantum-filtered (dqf)-COSY spectra with phase cycling were recorded on a Bruker AV400. A total of 32 scans were acquired using a time domain of 8 k in F2 (acquisition time of 1.2 s) and 512 increment in F1. Spectra were zero-filled to 8 k × 4 k prior to Fourier transformation and phasing using the Topspin software (Bruker). Heteronuclear HSQC and HMBC spectra were recorded using Bruker AMX500 with a cryoprobe. Samples were dissolved in 100 μl CD3OD using 2 mm NMR vials. For HSQC spectra, 40 scans were acquired using a time domain of 1 k in F2 and 256 increments in F1. For HMBC spectra, 256 scans were acquired using a time domain of 4 k in F2 and 128 increments in F1. Spectra were zero-filled to 4 k × 2 k prior to Fourier transformation and phasing using the Topspin software (Bruker).

Pauls G., Becker T., Rahfeld P., Gretscher R.R., Paetz C., Pasteels J., von Reuss S.H., Burse A, & Boland W. (2016). Two Defensive Lines in Juvenile Leaf Beetles; Esters of 3-nitropropionic Acid in the Hemolymph and Aposematic Warning. Journal of Chemical Ecology, 42, 240-248.

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Synthesis and Characterization of Mitochondria-Targeting PLLA Polymer

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Mitochondria-targeting PLLA (TPT polymer) was synthesized via ring opening polymerization and coupling reaction. The specific synthetic procedures are exhibited in the Supplementary Materials. Briefly, PLLA was synthesized by ring opening polymerization of LLA using Sn(Oct)₂ as a catalyst and 1,6-hexanediol (HI) as the initiator. Thereafter, the TPP was reacted with hydroxyl groups of PLLA to produce the TPT polymer. The structure of the synthesized polymer was characterized by ¹H NMR spectrum (AV-400, Bruker, USA) using CDCl₃ as the solvent, and 0.5% tetramethylsilane was used as the internal standard.
The synthesis of d-LND and HA-DOX was carried out using the coupling reaction method, in which the detailed synthesis procedures are shown in the Supplementary Materials. High-resolution mass spectrometer (LCMS-IT-TOF, Shimadzu, Japan) and ¹H NMR (AV-400, Bruker, America) were used for the structural verification.

He Y., Lei L., Cao J., Yang X., Cai S., Tong F., Huang D., Mei H., Luo K., Gao H., He B, & Peppas N.A. (2021). A combinational chemo-immune therapy using an enzyme-sensitive nanoplatform for dual-drug delivery to specific sites by cascade targeting. Science Advances, 7(6), eaba0776.

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NMR Characterization of POSS Compounds

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¹H spectra of Pep-1 (Nce₆POSS) and POSS-NH₂ were recorded on a Bruker AV400 instrument. The samples were dissolved in CDCl₃ at 1.0 mM. ²⁹Si NMR spectrum of Pep-1 was performed on a Bruker AV400 instrument with a higher concentration of 10.0 mM in dimethyl sulfoxide-d₆.

Wang M., Song Y., Zhang S., Zhang X., Cai X., Lin Y., De Yoreo J.J, & Chen C.L. (2021). Programmable two-dimensional nanocrystals assembled from POSS-containing peptoids as efficient artificial light-harvesting systems. Science Advances, 7(20), eabg1448.

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Synthesis of Organic Compounds

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All reactions were conducted
under an inert atmosphere of dry argon. Anhydrous 1,4-dioxane was
purchased from Aladdin and used without further purification. Unless
otherwise noted, the reagents and solvents used in this article were
all commercially available analytical or chemical grades and used
directly without any purification. The reactions were monitored by
thin-layer chromatography (TLC) on silica gel plates (GF 254) using
UV light to visualize the course of the reactions. Silica gel H (Qingdao
Sea Chemical Factory, Qingdao, People’s Republic of China)
was used for column chromatography.
¹H NMR spectra
were recorded on a Bruker AV 400 nuclear magnetic resonance instrument
(400 MHz). Chemical shifts were recorded in ppm relative to tetramethylsilane
as the internal standard. Data were reported as follows: chemical
shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet,
dd = doublet-doublet, dt = doublet-triplet, m = multiplet, br = broad),
coupling constants (Hz), and integration. ¹³C NMR data
were collected on a Bruker AV 400 nuclear magnetic resonance instrument
(100 MHz) with complete proton decoupling. Chemical shifts were reported
in ppm, with tetramethylsilane as the internal standard. The high
resolution electrospray ionization mass spectroscopy (HRESIMS) spectrum
was determined using a Waters ACQUITY UPLC/Xevo G2-S QTOF mass spectrometer.

Wan L.X., Zhen Y.Q., He Z.X., Zhang Y., Zhang L., Li X., Gao F, & Zhou X.L. (2021). Late-Stage Modification of Medicine: Pd-Catalyzed Direct Synthesis and Biological Evaluation of N-Aryltacrine Derivatives. ACS Omega, 6(14), 9960-9972.

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Inert Atmosphere Purification and NMR Characterization

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All reactions were carried out in a
glovebox under nitrogen atmosphere. Hexane, THF, toluene, and 1,4-dioxane
were dried by heating to reflux over sodium benzophenone ketyl and
then distilled under nitrogen prior to use. Chemicals were purchased
from Acros, Sigma-Aldrich, Alfa-Aesar, and Spectrochem, and used without
further purification. Mesitylene was used for the clarification of
product yield. The progress of reactions was monitored by Bruker AV-400
(¹H: 400 MHz, ¹³C: 101 MHz) using CDCl₃ as the solvent.

Xu X., Yan D., Zhu Z., Kang Z., Yao Y., Shen Q, & Xue M. (2019). Catalyst-Free Approach for Hydroboration of Carboxylic Acids under Mild Conditions. ACS Omega, 4(4), 6775-6783.

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Synthesis and Characterization of Homoserine Lactone

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All commercial materials were used without further purification. Flash chromatography was carried out using Macherey-Nagel (Hoerdt, France) Kieselgel 60 M silica. Analytical thin layer chromatography was realized using aluminum-backed plates coated with Macherey-Nagel Kieselgel 60 XtraSIL G/UV254. Compounds were visualized under UV light (at 254 nm) or stained using KMnO₄. Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker AVL300 or a Bruker AV400 or a Bruker AV500 spectrometer (Billerica, MA, USA), operating respectively at 300, 400, and 500 MHz for the proton (¹H) NMR. Carbon (¹³C) NMR spectra were recorded on a Bruker AVL300 or a Bruker AV400 spectrophotometer or a Bruker AV500 spectrometer, operating, respectively, at 75, 100, and 125 MHz. Chemical shifts were reported in parts per million (ppm) in the scale relative to residual solvent signals. Multiplicities are abbreviated as follows: s, singlet; d, doublet; t, triplet; dd, doublet of doublets; dt, doublet of triplts, m, multiplet; br, broad. Coupling constants were measured in Hertz (Hz). High-resolution mass spectra (HRMS) and low-resolution mass spectra were performed by the Centre Commun de Spectrométrie de Masse (CCSM), University of Lyon 1, Lyon, France. l- or d-homoserine lactone hydrobromide was synthesized as previously described [20 (link)].

Zhang Q., Queneau Y, & Soulère L. (2020). Biological Evaluation and Docking Studies of New Carbamate, Thiocarbamate, and Hydrazide Analogues of Acyl Homoserine Lactones as Vibrio fischeri-Quorum Sensing Modulators. Biomolecules, 10(3), 455.

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Analytical Characterization of Compounds

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All reagents were of commercial
grade and used as received unless indicated otherwise. The purity
of all tested compounds is >95% on the basis of liquid chromatography–mass
spectrometry (LC-MS) and nuclear magnetic resonance (NMR). ¹H- and ¹³C NMR spectra were recorded on a Bruker AV-400
(400 MHz), AV-600 (600 MHz), or AV-850 (850 MHz) spectrometer. Chemical
shifts are given in ppm (δ) relative to CD₃OD or
CDCl₃ as an internal standard. Coupling constants are given
in Hz, and peak assignments are based on 2D ¹H correlation
spectroscopy and ¹³C heteronuclear single quantum coherence
NMR experiments. All ¹³C attached proton test spectra are
proton-decoupled. LC-MS analysis was performed on a Finnigan Surveyor
high-performance liquid chromatography (HPLC) system with a Gemini
C18 50 × 4.60 mm column (detection at 200–600 nm) coupled
to a Finnigan LCQ Advantage Max mass spectrometer with electrospray
ionization (ESI). Methods used are: 15 min (0–0.5 min: 10%
MeCN; 0.5–10.5 min: 10–90% MeCN; 10.5–12.5 min:
90% MeCN; 12.5–15 min: 90–10% MeCN) or 12.5 min (0–0.5
min: 10% MeCN; 0.5–8.5 min: 10–90% MeCN; 8.5–10.5
min: 90% MeCN; 10.5–12.5 min: 90––10% MeCN).
HRMS was recorded on an LTQ Orbitrap (ThermoFinnigan). For reverse-phase
HPLC purification, an automated Gilson HPLC system equipped with a
C18 semiprep column (Phenomenex Gemini C18, 5 μm 250 ×
10 mm) and a GX281 fraction collector was used.

Xin B.T., Huber E.M., de Bruin G., Heinemeyer W., Maurits E., Espinal C., Du Y., Janssens M., Weyburne E.S., Kisselev A.F., Florea B.I., Driessen C., van der Marel G.A., Groll M, & Overkleeft H.S. (2019). Structure-Based Design of Inhibitors Selective for Human Proteasome β2c or β2i Subunits. Journal of Medicinal Chemistry, 62(3), 1626-1642.

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Optimized Synthesis of Heterocyclic Compounds

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All reactions were carried out employing standard chemical techniques under inert atmosphere. Solvents used for extraction, washing, and chromatography were HPLC grade. All reagents were purchased from commercial sources and were used without further purification. Analytical HPLC was performed on an Agilent 1200 LCMS with UV detection at 215 nm along with ELSD detection and electrospray ionization, with all final compounds showing >95% purity and a parent mass ion consistent with the desired structure. All NMR spectra were recorded on a 400 MHz Brüker AV-400 instrument. ¹H chemical shifts are reported as δ values in ppm relative to the residual solvent peak (CDCl₃ = 7.26). Data are reported as follows: chemical shift, multiplicity (br = broad, s = singlet, d = doublet, t = triplet, q = quartet, dd = doublet of doublets, m = multiplet), coupling constant (Hz), and integration. ¹³C chemical shifts are reported as δ values in ppm relative to the residual solvent peak (CDCl₃ = 77.16). High-resolution mass spectra were obtained on an Agilent 6540 UHD Q-TOF with ESI source. Automated flash column chromatography was performed on an Teledyne ISCO Combi-Flash system. Melting points were recorded on an OptiMelt automated melting point system by Stanford Research Systems.

Childress E.S., Garrison A.T., Sheldon J.R., Skaar E.P, & Lindsley C.W. (2019). Total Synthesis of Hinduchelins A–D, Stereochemical Revision of Hinduchelin A, and Biological Evaluation of Natural and Unnatural Analogues. The Journal of organic chemistry, 84(10), 6459-6464.

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