Penicillin streptomycin solution

Manufactured by Sangon

Sourced in China, United States

Penicillin/streptomycin solution is a sterile, liquid mixture of the antibiotics penicillin and streptomycin. It is commonly used in cell culture applications to prevent bacterial contamination.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (7 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (3 mentions) Aprepitant, by MedChemExpress (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Sangon (2 mentions) Hek293t, by American Type Culture Collection (2 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Hfdpc, by PromoCell (1 mentions) Osimertinib, by Selleck Chemicals (1 mentions) Gefitinib, by Selleck Chemicals (1 mentions) Hcc827, by American Type Culture Collection (1 mentions) Nci h226, by American Type Culture Collection (1 mentions) Nci h1975, by American Type Culture Collection (1 mentions) Nci h1944, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (1 mentions) C11995500bt, by Sartorius (1 mentions) Dulbecco modified eagle medium (dmem), by Keygen Biotech (1 mentions) Cmt93 cells, by American Type Culture Collection (1 mentions) Ln229, by Thermo Fisher Scientific (1 mentions)

20 protocols using penicillin streptomycin solution

Wnt10B Modulation of Dermal Papilla Cells

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HFDPCs isolated from human dermis originating from the lateral scalp were purchased from PromoCell GmbH. Cells were cultured in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.; 10099-14-FBS) and 1% Penicillin-Streptomycin Solution (E607011, Sangon Biotech Co., Ltd.), at 37°C and 5% CO₂. Human recombinant WNT10B protein was purchased from R&D Systems (cat. no. 7196-WN). The WNT10B treatment conditions used in the present study were as previously described (38 (link)). WNT10B protein was reconstituted in PBS containing 0.1% bovine serum albumin (BSA; Sangon Biotech Co., Ltd.; A602440) to form a solution of 10 µg/ml and used at a final concentration of 1 µg/ml. The expression of the Wnt target gene β-catenin in DPCs was increased following treatment with 1 µg/ml WNT10B protein for 3 days at 37°C, which was in agreement with the results of a previous study (25 (link)) indicating that the culture conditions were appropriate.
For RNA-sequencing (RNA-seq), the DPCs were divided into two groups: i) The experimental group, in which DPCs were cultured in DMEM supplemented with 10% FBS and 1 µg/ml recombinant WNT10B for 3 days at 37°C; and ii) the control group, in which DPCs were cultured in DMEM containing 10% FBS and 1 µg/ml BSA for 3 days at 37°C.

Zhou Q., Song Y., Zheng Q., Han R, & Cheng H. (2019). Expression profile analysis of dermal papilla cells mRNA in response to WNT10B treatment. Experimental and Therapeutic Medicine, 19(2), 1017-1023.

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NSCLC Cell Line Characterization

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Human NSCLC cell lines NCI-H1975, NCI-H1944, NCI-H226, A549, HCC827 were purchased from American Type culture collection (ATCC, Manassas, VA, USA) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (ThermoFisher, Waltham, USA) and 1% penicillin/streptomycin solution (Sangon, Shanghai, China). The cells were cultured in a humidified incubator at 37 °C with 5% CO₂. EGFR genotyping was performed in each cell line according to the instruction of the manufacture (Yihe, Shanghai, China). Aprepitant was obtained from MedChemExpress (NJ, USA). Osimertinib and gefitinib were from Selleck Chemicals (Houston, TX, USA). All chemicals were dissolved in dimethyl sulfoxide at the concentration of 100 mM and aliquoted and stored at −20 °C until use. Human hemokinin-1 (hHK-1) peptide (TGKASQFFGLM-NH₂) and substance P (SP) (RPKPQQFFGLM-NH2) were synthesized by Fmoc solid-phase synthesis system as described before [28 (link)]. The molecular weight of the peptide was confirmed by ESI-TOF mass spectrometry. The purity of peptide was quantified to be >95% using reversed-phase HPLC by a C18 column as the solid phase and a H₂O:acetonitrile gradient as the liquid phase.

Zhang X.W., Li L., Hu W.Q., Hu M.N., Tao Y., Hu H., Miao X.K., Yang W.L., Zhu Q, & Mou L.Y. (2022). Neurokinin-1 receptor promotes non-small cell lung cancer progression through transactivation of EGFR. Cell Death & Disease, 13(1), 41.

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Senescence model in NIH/3T3 cells

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Mouse embryonic fibroblast NIH/3T3 cells were provided by our laboratory and used to build the senescence model. NIH/3T3 cells were cultured in DMEM (Dulbecco’s modified Eagle’s medium) (ATCC, Cambridge, MA, USA) supplemented with 10% foetal bovine serum (ThermoFisher Scientific, Waltham, MA, USA) and 1% penicillin/streptomycin solution (Sangon Biotech, Shanghai, China) in a 37 °C, 5% CO₂ incubator. Cell transfection was performed according to the LipoMaxTM transfection reagent (Sudgen, Nanjing, China) instruction manual, and the transfection concentrations of mimics, inhibitors, and negative control were all 20 pmol/μL. NIH/3T3 cells in their exponential growth phase were treated with 0.5 μM adriamycin (ADR) (Merck, Darmstadt, Germany). After 72 h, the cells were collected and used to confirm cellular senescence. The mimics, inhibitors, and negative control of tRF-Trp-CCA-014 were designed and synthesized by Sangon Biotech Co., Ltd. Shanghai, China.

Yang D., Xiao F., Yuan Y., Li J., Wang S., Fan X., Ni Q., Li Y., Zhang M., Gu X., Yan T., Yang M, & He Z. (2023). The Expression Pattern of tRNA-Derived Small RNAs in Adult Drosophila and the Function of tRF-Trp-CCA-014-H3C4 Network Analysis. International Journal of Molecular Sciences, 24(7), 6169.

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Engineered HEK293F Cell Lines for DNA Methylation Studies

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All the HEK293F-derived cells are maintained in Dulbecco’s Modified Eagle Medium (Gibco C11995500BT) with 10% fetal bovine serum (Biological Industries 04-010-1ACS 500 mL) and 1× penicillin streptomycin solution (Sangon Biotech, China E607011-0100) using a cell culture incubator at 37°C and 5% CO₂. The HEK293F cells stably inserted with a DNA methylation silenced CMV reporter (Figure 1A) and its TET1 KO, TET2 KO, TET3 KO, TET TKO, and RELA KO cells are previously established and described (Li et al., 2018b (link); Zhao et al., 2019 (link)). The MER11B-left KO, MER11B-right KO, MER11B-left+right KO, and ERV KO cells generated in this study are also derived from the HEK293F cells stably inserted with the DNA methylation silenced CMV reporter. The cell lines used in this study are free from mycoplasma contamination and authenticated: The HEK293F cells stably inserted with a DNA methylation silenced CMV reporter is established in Dr. Bing Zhu’s lab and widely used in our previous studies (Dong et al., 2018 (link); Du et al., 2019 (link); Li et al., 2018a (link); Li et al., 2018b (link); Zhao et al., 2019 (link)); the flow cytometry results (Figure 1C) can confirm the insertion of DNA methylation-silenced CMV reporter.

Zhao Z., Zhang Z., Li J., Dong Q., Xiong J., Li Y., Lan M., Li G, & Zhu B. (2020). Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness. eLife, 9, e61965.

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CMT93 Cell Culture Conditions

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CMT93 cells were originally obtained from American Type Culture Collection (Virginia, USA). The cells were maintained in DMEM (KeyGENBioTECH, Nanjing, China) containing 10% fetal bovine serum and 1% penicillin-streptomycin solution (Sangon Biotech, Shanghai, China), and cultured at 37°C with 5% CO₂.

Fu L., Lin W., Wang C, & Wang Y. (2022). Establishment of a 3-Dimensional Intestinal Cell Model to Simulate the Intestinal Mucosal Immune System for Food Allergy Investigations. Frontiers in Immunology, 13, 853443.

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Glioma Cell Lines Transfection Protocol

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Human glioma cell lines (LN229, SHG44, U251, and T98G) and normal glial cell line (HEB) were obtained from Biovector National Typical Culture Center (NTCC, Beijing, China). Glioma cell lines were cultured in DMEM medium and HEB cells were grown in RPMI-1640 medium (all from Gibco, Grand Island, NJ, USA). Both mediums were supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% Penicillin-Streptomycin Solution (Sangon Biotech, Shanghai, China). All cells were cultured in an incubator at 37°C with 5% CO₂.
For cell transfection, all oligonucleotides and vectors were synthesized by Ribobio (Guangzhou, China), including the small interference RNA (siRNA) for circ_0030018 (si-circ_0030018: 5′-GAATGAAATTAGTTGTCACTG-3′) and TRIM44 (si-TRIM44: 5′-AAUAGGUACUCAAAUCAAGCC-3′), or circ_0030018 lentiviral short hairpin RNA (sh-circ_0030018: 5′-CCGGAAATTAGTTGTCACTGTTAATCTCGAGATTAACAGTGACAACTAATTTTTTTTG-3′), miR-194-5p mimic or inhibitor (miR-194-5p or in-miR-194-5p), the pcDNA overexpression vector of TRIM44, and their controls (si-circ-NC, si-NC, sh-NC, miR-NC, in-miR-NC, or vector). Lipofectamine 3000 Reagent (Invitrogen, Carlsbad, CA, USA) was used to transfect them into LN229 and U251 cells.

Shao Y., Yang Z., Miao W., Yu X., Wu Y, & Pu Y. (2021). circ_0030018 promotes glioma proliferation and metastasis. Translational Neuroscience, 12(1), 260-272.

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Human VSMC Culture Protocol

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Human primary VSMCs were purchased from Bluefcell Bio and maintained in DMEM supplemented with 10% FBS (both from Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin solution (Sangon Biotech Co. Ltd.) at 37˚C and 5% CO₂. The use of VSMCs was approved by the Ethics Committee of Affiliated Hospital of Inner Mongolia Medical University with approval number KY (2020015).

Zheng H, & Bai L. (2023). MALT1 accelerates proatherogenic vascular smooth muscle cell growth, invasion and synthetic phenotype switching via nuclear factor‑κB signaling‑dependent way. Experimental and Therapeutic Medicine, 26(1), 337.

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Transfection of HEK293 and HeLa Cells

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HEK293 [American Type Culture Collection (ATCC)] and Hela (ATCC) cells were cultured in DMEM (Sangon Biotech) supplemented with 10% (v/v) FBS (Sangon Biotech) and 1% (v/v) penicillin-streptomycin solution (Sangon Biotech) in a 5% CO₂ atmosphere at 37 °C and passaged every 3 days. At 70–80% confluence, cells were detached with 1 mL of 0.25% trypsin solution (Sangon Biotech) followed by addition of 5 mL of DMEM to neutralize the trypsin. Around 20,000 cells were further cultured for 36 h before transfection. Transfection was accomplished using the Lipofectamine 3000 reagent (Thermo Fisher) mixed with 1 μg of the plasmid, pCAGGS::RGG-mfp-3-RGG-mCherry or pcDNA::RGG-gfp-RGG, per dish, according to the manufacturer’s transfection protocol. Transfected cells were further incubated under the standard culturing condition for 12–16 h before imaging.

Li M., Park B.M., Dai X., Xu Y., Huang J, & Sun F. (2022). Controlling synthetic membraneless organelles by a red-light-dependent singlet oxygen-generating protein. Nature Communications, 13, 3197.

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Cell Culture Protocol for Cell Lines

Cited 1 time

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Human fibroblasts (HFBS) were a gift from Prof. Shichu Xiao [42 (link)]. L929 and RAW 264.7 macrophage cell lines were from Meilian Biotech Company (China) and National Collection of Authenticated Cell Cultures (China), respectively. Human umbilical vein endothelial cells (HUVECs) were from the China Center for Type Culture Collection (China). All cell lines were cultured with high glucose DMEM medium supplemented with 10% FBS (Gibco, USA) and 100 units/ml Penicillin/Streptomycin Solution (Sangon Biotech, China) under 5% CO₂ at 37°C in a humidified incubator (ThermoFisher, USA).

Wu C., Zhou Z., You X., Guo Y., Chen P., Li H, & Tong X. (2022). Tannic acid-loaded hydrogel coating endues polypropylene mesh with hemostatic and anti-inflammatory capacity for facilitating pelvic floor repair. Regenerative Biomaterials, 9, rbac074.

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Neuroinflammation Inhibition Assay

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The agonist PNU282987 and antagonist MLA were purchased from Tocris Bioscience (Bristol, UK), and LPS was purchased from Sigma–Aldrich (St. Louis, MO, USA). The [A10L]PnIA linear crude peptide was synthesized by Bankpeptide Biological Technology Co., Ltd. (Hefei, China) and purified in our laboratory.
A CCK-8 kit, DMEM-H medium, Penicillin/Streptomycin Solution, and PBS buffer were purchased from Sangon Biotech (Shanghai, China). FBS was purchased from Procell (Wuhan, China). A FastPure Cell/Tissue Total RNA Isolation Kit, HiScript II Q Select RT SuperMix for qPCR (+gDNA wiper), 2 × Taq Plus Master Mix Ⅱ, and ChamQ Universal SYBR qPCR Master Mix were obtained from Vazyme (Nanjing, China). A TNF-α ELISA kit, IL-6 ELISA kit, and IL-1β ELISA kit were purchased from Thermo Scientific (Waltham, MA, USA).

Tan Y., Chu Z., Shan H., Zhangsun D., Zhu X, & Luo S. (2022). Inflammation Regulation via an Agonist and Antagonists of α7 Nicotinic Acetylcholine Receptors in RAW264.7 Macrophages. Marine Drugs, 20(3), 200.

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