Sw22 shaking water bath

Manufactured by Julabo

Sourced in Germany

The Julabo SW22 Shaking Water Bath is a laboratory equipment designed for temperature-controlled incubation, shaking, and mixing applications. It features a stainless steel bath with a temperature range of ambient +5°C to 99.9°C, controlled by a microprocessor-based temperature control system. The shaking mechanism provides adjustable speeds from 20 to 200 rpm, allowing for efficient sample agitation. The SW22 Shaking Water Bath is suitable for a variety of laboratory tasks that require precise temperature regulation and gentle mixing.

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Lab products found in correlation

Elmasonic s100h, by Elma Schmidbauer (1 mentions) Whatman, by Cytiva (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Ammonium formate, by Merck Group (1 mentions) Milli q plus, by Merck Group (1 mentions) Agilent 1200 hplc, by Agilent Technologies (1 mentions) Formic acid, by Merck Group (1 mentions) Agilent 6410 triple quadrupole, by Agilent Technologies (1 mentions) 0.2 m syringe filter, by Avantor (1 mentions) Lambda 35 uv vis spectrophotometer, by PerkinElmer (1 mentions)

Spelling variants of this product

Water bath (25 citations) Shaking water bath (6 citations)

7 protocols using sw22 shaking water bath

Chromium Adsorption Isotherms Protocol

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Adsorption isotherms were obtained from batch tests conducted in 500 mL Erlenmeyer flask using a JULABO’s digital SW22 Shaking Water Bath (JULABO GmbH, Seelbach, Germany). The sorbent weight was 2 g (i.e., m/V = 4 g L⁻¹), the temperature was 23 °C, and the initial Cr(VI) concentration varied from 15 to 700 mg L⁻¹. K₂Cr₂O₇ was used as the Cr(VI) source.
The pH of the solutions was set to two by utilizing diluted H₂SO₄. The jugs were fixed and mechanically tumbled for seven days. This timeframe was chosen following the initial investigations to accomplish the equilibrium conditions.
The utilization of adsorption isotherms is extremely helpful in investigating the interaction between the adsorbate and adsorbent in any framework. The parameters received from diverse models provide vital information on the surface properties and affinities of the adsorbent. There are several conditions for breaking down exploratory adsorption equilibrium information, and the best known surface adsorption models for single solute frameworks are the Langmuir and Freundlich models.

Politi D, & Sidiras D. (2020). Modified Spruce Sawdust for Sorption of Hexavalent Chromium in Batch Systems and Fixed-Bed Columns. Molecules, 25(21), 5156.

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Comparative Evaluation of NADES and Conventional Solvents for Plant Extraction

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The plant material was extracted in 100 mL Erlenmeyer flasks at 50 °C using two distinct techniques—maceration (M) and ultrasound-assisted extraction (UAE)—and different NADES combinations (Table 1) with a plant/solvent proportion of 0.25:10 (w/v). With the aim to compare extraction efficiency of NADES and conventional solvents, water, ethanol 80% (EtOH 80) and methanol (MeOH) were also tested as extractant solvents. For maceration, the extraction was performed in a SW22 Shaking Water Bath (Julabo, Seelbach, Germany) at 200 rpm for 60 min. Regarding UAE, an Elmasonic S 100 H (220–240 V, 550 W) ultrasound bath (Elma Hans Schmidbauer GmbH & Co. KG, Singen, Germany) with 9 L of water was used at a frequency of 37 kHz (in sweep-function) at different extraction periods (15, 30 and 60 min). Since flask positioning in the ultrasound bath has been shown to affect the extraction efficiency [19 (link)], during extraction procedure all Erlenmeyer flasks were kept in the same position and the water was kept above the level of the solvent in the flasks. All extracts were filtered through Whatman nº. 1 filter paper (Whatman Int. Ltd., Maidstone, England) and the filtrates were stored at −20 °C until use.

Mansinhos I., Gonçalves S., Rodríguez-Solana R., Ordóñez-Díaz J.L., Moreno-Rojas J.M, & Romano A. (2021). Ultrasonic-Assisted Extraction and Natural Deep Eutectic Solvents Combination: A Green Strategy to Improve the Recovery of Phenolic Compounds from Lavandula pedunculata subsp. lusitanica (Chaytor) Franco. Antioxidants, 10(4), 582.

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In Vitro Release of Paclitaxel from Polymeric Micelles

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Release of PTX
from MM-PCL₁₅, MM-PCL₂₂, or MM-PBCL₂₂ and micelles prepared from individual PEO-PCLs or PEO-PBCL or PEG-DSPE
polymer was determined in 0.01 M phosphate buffer (pH 7.4) containing
10% fetal bovine serum (FBS). The micellar solution (3 mL) was transferred
into a dialysis bag (Spectrapor, MW cut-off 3500 Da) and placed into
500 mL of 0.01 M phosphate buffer (pH 7.4) with 10% FBS. The release
study was performed at 37 °C in a Julabo SW 22 shaking water
bath (Germany). At selected time intervals, the whole release media
has been replaced with a fresh one and aliquots of 200 μL were
withdrawn from the inside of the dialysis bag for HPLC analysis and
replaced with double distilled water. The amount of PTX released was
calculated by subtracting the amount of PTX remaining in the dialysis
bag from the initial amount of PTX. The release profiles were compared
using the similarity factor, f₂, and the
profiles were considered significantly different if f₂ < 50. where n is the sampling
number
and R_j and T_j are the percent released of the reference and test
formulations at each time point “j”.

Saqr A., Vakili M.R., Huang Y.H., Lai R, & Lavasanifar A. (2019). Development of Traceable Rituximab-Modified PEO-Polyester Micelles by Postinsertion of PEG-phospholipids for Targeting of B-cell Lymphoma. ACS Omega, 4(20), 18867-18879.

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Comparative Release Kinetics of Cisplatin Formulations

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The release of free cisplatin and its micellar formulations (plain and GE11 cisplatin micelles) was measured using equilibrium dialysis method in PBS (pH = 7) and acetate buffer saline (pH = 5). Briefly, free cisplatin, plain or GE11 cisplatin micelles (4 mL) containing 30 µg/mL cisplatin were placed into a dialysis bag (Spectrapor, MWCO 3500) in a beaker containing 500 mL PBS or acetate buffer saline. The release study was performed at 37 °C in a Julabo SW 22 shaking water bath (Seelbach, Germany). At selected time intervals, 100 μL samples were withdrawn from the inside of dialysis bag and replaced with fresh medium for ICP-MS analysis. The percent cumulative amount of cisplatin released was calculated and plotted as a function of time. The release profiles of plain and GE11 cisplatin micelles were compared using the similarity factor, f₂, and the profiles were considered significantly different if f₂ < 50 [37 (link)]. The similarity factor, f₂, was calculated using the following equation [38 (link)].

f_{2} = 50 \times \log ({[1 + (\frac{1}{n}) \sum_{j = 1}^{n} {| R j - T j |}^{2}]}^{- 0.5} \times 100)

where n is the sampling number, R_j and T_j are the percents released of the reference and test formulations at each time point j.

Soleymani Abyaneh H., Soleimani A.H., Vakili M.R., Soudy R., Kaur K., Cuda F., Tavassoli A, & Lavasanifar A. (2018). Modulation of Hypoxia-Induced Chemoresistance to Polymeric Micellar Cisplatin: The Effect of Ligand Modification of Micellar Carrier Versus Inhibition of the Mediators of Drug Resistance. Pharmaceutics, 10(4), 196.

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In Vitro Metabolism of Ellagic Acid Sulfate

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HLMs (product number: M0567), collected from various male human livers, were obtained from Sigma-Aldrich (St. Louis, Missouri, USA) and stored at −70°C until use. Organic solvents and reference powders were of HPLC grade and analytical grade (AR), respectively. HPLC grade water was prepared in-house by a Milli-Q Plus filtration instrument (Millipore, USA). ESB (98.51%) and NADPH (99.99%) were obtained from MedChemExpress (USA). Acetonitrile, formic acid, ammonium formate, GSH, and KCN were acquired from Sigma-Aldrich (USA). LC-MS/MS analysis was performed using Agilent 1200 HPLC connected to Agilent 6410 triple quadrupole. Electrospray ionization (ESI) was used as an interface. Incubation of ESB with HLMs was performed in the SW22 Shaking Water Bath (JULABO, Seelbach, Germany) adjusted at 37^°C.

Abdelhameed A.S., Attwa M.W, & Kadi A.A. (2020). Characterization of Stable and Reactive Metabolites of the Anticancer Drug, Ensartinib, in Human Liver Microsomes Using LC-MS/MS: An in silico and Practical Bioactivation Approach. Drug Design, Development and Therapy, 14, 5259-5273.

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Solubility Determination of SLM and LNT

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An excess amount of pure SLM and optimized LNT were dispersed in the dissolution medium and placed on a shaker (Shaking Water Bath SW22, JULABO GmbH, Germany) at 37 •C ± 0.5
•C. the shaking was hold on till the solubility equilibrium had been reached. Samples were filtered through 0.2 µm syringe filter (VWR, Leuven). Then the concentration of SLM was spectrophotometerly analyzed (Lambda 35 UV/VIS Spectrophotometer, PerkinElmer, Singapore) at 288 nm. The measurements were repeated three times. In addition, one-way ANOVA test was carried out to detect the significant difference (p value) between pure drug and optimized LNT.

Ibrahim A.H., Rosqvist E., Smått J.H., Ibrahim H.M., Ismael H.R., Afouna M.I., Samy A.M, & Rosenholm J.M. (2019). Formulation and optimization of lyophilized nanosuspension tablets to improve the physicochemical properties and provide immediate release of silymarin. International journal of pharmaceutics, 563.

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Kinetics of PR and Hemoglobin Release

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To exploit the release kinetics of PR–CNG–ER, and hemoglobin from carrier erythrocytes, 0.5 ml of packed PR–CNG–ER was diluted to 5 ml using ringer solution. The suspension was mixed thoroughly by several gentle inversions and, then, placed in Shaking Water Bath: SW22 (Julabo Labortechnik GmbH, Seelbach, Germany) set at 150 rpm and 37 °C. After 0.25, 0.5, 1, 2, 4, 6, 8, 12, 24 and 48 h incubation, one of the aliquots was harvested, 200 μl of the supernatants were extracted by 500 μl of methanol through vortexing and centrifuging at 13,000 rpm for 30 min, then, injected directly to chromatograph for PR assay. In addition, the absorbance of another 200 μl portion of the supernatant diluted with 1 ml of PBS was determined at 540 nm using a UV/visible spectrophotometer to monitor the hemoglobin release. The initial volume of release medium was maintained by refilling 200 μl of the ringer solution after each withdrawal. These experiments were carried out in triplicate (Hamidi et al., 2007 (link); Hamidi et al., 2011 (link)).

Harisa G.I., Badran M.M., AlQahtani S.A., Alanazi F.K, & Attia S.M. (2015). Pravastatin chitosan nanogels-loaded erythrocytes as a new delivery strategy for targeting liver cancer. Saudi Pharmaceutical Journal : SPJ, 24(1), 74-81.

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