Heart tissues were fixed with 4% paraformaldehyde overnight and then embedded in paraffin. Expressions of nitrotyrosine, TNF-α, TGF-β1, and collagen I were examined on the tissue section using immunohistochemical (IHC) analyses as described previously (Tian et al., 2009 (link)). The dilution concentration of nitrotyrosine antibody (Millipore, Burlington, MA, United States), tumor necrosis factor α (TNF-α) antibody (Abcam, Cambs, United Kingdom), transforming growth factor β1 (TGF-β1) antibody (Abcam, Cambs, United Kingdom), collagen I antibody (Abcam, Cambs, United Kingdom), and CD31 (Abcam, Cambs, United Kingdom) was 1:100, 1:50, 1:200, and 1:300, 1:500, respectively. For IHC analysis, each section was captured in at least 10 pictures and all pictures were quantified. The protein expression intensity was assessed by estimating the area of the objects and the medium pixel intensity per object, as the integrated optical density (IOD). IOD to area ratio was used to present the results. All images were acquired and processed in TIFF format, analysis was done using Image Pro Plus 6.0 software (Media Cybernetics, Rockville, MD, United States). Capillary density was assessed by capillaries/myocyte nucleus (C/M) values. C/M values were counted in 5 fields randomly selected from each slice.
Transforming growth factor β1 tgf β1 antibody
Manufactured by Abcam
Sourced in United Kingdom
Transforming growth factor β1 (TGF-β1) antibody is a laboratory reagent used to detect and measure the TGF-β1 protein in various biological samples. TGF-β1 is a multifunctional cytokine that plays a role in cell growth, differentiation, and immune function.
Automatically generated - may contain errors
2 protocols using transforming growth factor β1 tgf β1 antibody
1
Cardiac Tissue Immunohistochemistry Analysis
2
Western Blotting of TGF-β1 in Tissue Homogenates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CC tissue homogenates were used for Western blotting performed according to standard procedures. Briefly, homogenates were lysed in lysis buffer containing 50mM Tris–HCl (pH 8.0). These homogenates were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis, transferred onto a polyvinylidene fluoride membrane blocked with 5% skim milk, and hybridized with primary antibodies. Transforming growth factor-β1 (TGF-β1) antibody and monoclonal β-actin antibody were purchased from Abcam. After incubation with horseradish-peroxidase-conjugated secondary antibody at room temperature, immunoreactive proteins were detected using a chemiluminescent electrogenerated chemiluminescence assay kit (Abcam) according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!