Rj nmri nude mice

Four-week-old female Rj:NMRI-nude mice (Janvier, Le Genest Saint Isle, France) were maintained under pathogen-free conditions at the Experimental Therapy Unit (Faculty of Medicine, Nantes, France) in accordance with the institutional guidelines of the French Ethical Committee (CEEA Pays de la Loire n°06: project authorization 8405). Mice received an intramuscular injection of 1.10⁶ K-HOS parental or mutant cells in close proximity to the tibia. Five days after cell injections, groups of mice received or not the molecules verteporfin (20 mg/kg), CA3 (10 mg/kg) or control vehicle twice a week by intraperitoneal injection. The tumor volume (V) was calculated from the measurement of three perpendicular diameters using a caliper according to the following formula: V = (length × height × depth)/2.

Four-week-old Rj:NMRI-nude mice (Janvier labs—Le Genest-Saint-Isle, France) were housed under pathogen-free conditions at the Experimental Therapeutics Unit (Medical school, Nantes, France). The mice were anesthetized by inhalation of an isoflurane/air mixture before receiving an intramuscular injection of 1 × 10⁶ TC-71 Ewing sarcoma cells or K-HOS osteosarcoma cells in close proximity to the tibia. The tumor volume (V) was calculated from the measurement of three perpendicular diameters using a caliper, according to the following formula: V = 0.5 × length × width × height. Mice were sacrificed when tumor volume reached 2000 mm³ for ethical reason. For consistency across the experiments shown here, the arbitrary limit of 1200 mm³ was assigned for Kaplan–Meier estimator plots.