We studied 19 patients who were diagnosed with urothelial BC who received TURBT (13 NMIBC and six MIBC; 18 males; and 3 females). Informed consent was obtained from all patients. This study was approved by the Institutional Review Board of clinical research and genome research committee at Yamanashi Central Hospital (G-2018-1) and complied with Declaration of Helsinki principles. Peripheral blood samples were obtained and centrifuged to separate buffy coats (12 (link)). Urine was also centrifuged and separate into urine precipitate and supernatant. Buffy coat, urine precipitate, and urine supernatants were stored at −80°C until DNA extraction. Buffy coat was used as a control to detect somatic mutations in tumor tissues. DNA from buffy coat and urine precipitate was extracted with the QIAamp DNA Blood Mini QIAcube Kit (Qiagen, Hilden, Germany) and the DNA concentration was determined using a Nano Drop 2000 (Thermo Fisher Scientific, Waltham, MA, United States). DNA was extracted from the urine supernatant with the MagMax Cell Free DNA extraction kit and KingFisher Duo Prime (Thermo Fisher Scientific). DNA from urine supernatant was determined using the Qubit dsDNA HS Assay Kit and Qubit 3.0 fluorometer (Thermo Fisher Scientific) according to the manufacturer’s instructions.
Magmax cell free dna extraction kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MagMax Cell Free DNA extraction kit is a lab equipment product designed for the isolation and purification of cell-free DNA (cfDNA) from various sample types. It utilizes magnetic bead-based technology to efficiently capture and extract cfDNA, providing a reliable and consistent method for obtaining this analyte.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop 2000, by Thermo Fisher Scientific (6 mentions)
Qubit 3.0 fluorometer, by Thermo Fisher Scientific (6 mentions)
Qubit dsdna hs assay kit, by Thermo Fisher Scientific (4 mentions)
Qiaamp dna blood mini qiacube kit, by Qiagen (3 mentions)
Kingfisher duo prime, by Thermo Fisher Scientific (3 mentions)
Qubit 3 fluorometer, by Thermo Fisher Scientific (1 mentions)
Magmax ffpe dna rna ultra kit, by Thermo Fisher Scientific (1 mentions)
Generead dna ffpe kit, by Qiagen (1 mentions)
Qiaamp dna ffpe tissue kit, by Qiagen (1 mentions)
4 protocols using magmax cell free dna extraction kit
1
Urothelial Bladder Cancer DNA Analysis
2
Urine and Blood DNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This study included 25 patients who were diagnosed with BC by transurethral resection of bladder tumor (TURBT), four patients with cystitis and one patient with inverted papilloma (25 males and 5 females; age 50‐90 years) (Table S1 ). Informed consent was obtained from all patients. This study was approved by the Institutional Review Board at Yamanashi Central Hospital. Urine and blood samples were obtained before TURBT. Urine precipitate (cellular fraction) and urine supernatant (non‐cellular fraction) were collected after centrifugation (Figure 1 ).18 DNA was extracted from the buffy coat and urine precipitation with the QIAamp DNA Blood Mini QIAcube Kit (Qiagen, Hilden, Germany) and DNA concentration was determined using Nano Drop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). DNA was extracted from the urine supernatant and plasma with the MagMax Cell Free DNA extraction kit and the KingFisher Duo Prime (Thermo Fisher Scientific).
3
Cell-free DNA Isolation from Liquid Biopsy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Isolation of cell-free DNA was performed using the bead-based MagMAX™ Cell-free DNA extraction kit (catalog no.: A36716, Thermo Fisher Scientific, Waltham, MA, USA), specialized for high-quality isolation and specific enrichment of nucleic acids from liquid biopsies, as previously reported [11 (link)].
4
DNA Extraction from Blood and Tumor Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood samples were collected and centrifuged to separate buffy coats and plasma. Buffy coats were stored at − 80 °C until DNA extraction. Buffy coat DNA was extracted with the QIAamp DNA Blood Mini QIAcube Kit (Qiagen, Hilden, Germany). The DNA concentration of buffy coats was determined using a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA). Plasma DNA was extracted with the MagMax Cell-Free DNA extraction kit on the KingFisher Duo Prime (Thermo Fisher Scientific). Concentration of plasma DNA was determined using the Qubit dsDNA HS Assay Kit and Qubit 3.0 fluorometer (Thermo Fisher Scientific) according to the manufacturer’s instructions.
Tumor tissues were fixed using 10% buffered formalin29 (link). Serial 10 μm sections were prepared from formalin-fixed paraffin-embedded (FFPE) tissues, and sections were stained with hematoxylin–eosin and reviewed by a pathologist to check the tumor area. Laser capture microdissection was performed using an Arcturus XT laser microdissection system (Thermo Fisher Scientific). FFPE DNA was extracted using the QIAamp DNA FFPE Tissue Kit (Qiagen), the GeneRead DNA FFPE Kit (Qiagen), and the MagMAX™ FFPE DNA/RNA Ultra Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. FFPE DNA concentrations were determined using the Qubit® dsDNA HS Assay Kit on a Qubit Fluorometer 3.0 (Thermo Fisher Scientific).
Tumor tissues were fixed using 10% buffered formalin29 (link). Serial 10 μm sections were prepared from formalin-fixed paraffin-embedded (FFPE) tissues, and sections were stained with hematoxylin–eosin and reviewed by a pathologist to check the tumor area. Laser capture microdissection was performed using an Arcturus XT laser microdissection system (Thermo Fisher Scientific). FFPE DNA was extracted using the QIAamp DNA FFPE Tissue Kit (Qiagen), the GeneRead DNA FFPE Kit (Qiagen), and the MagMAX™ FFPE DNA/RNA Ultra Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. FFPE DNA concentrations were determined using the Qubit® dsDNA HS Assay Kit on a Qubit Fluorometer 3.0 (Thermo Fisher Scientific).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!