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Vascular cell basal medium

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Vascular Cell Basal Medium is a cell culture medium designed to support the growth and maintenance of vascular cells, such as endothelial and smooth muscle cells. It provides the necessary nutrients and growth factors required for these cell types to thrive in an in vitro environment.

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Lab products found in correlation

Endothelial cell growth kit vegf, by American Type Culture Collection (6 mentions) Endothelial cell growth kit bbe, by American Type Culture Collection (5 mentions) Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (4 mentions) Microvascular endothelial cell growth kit vegf, by American Type Culture Collection (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Vascular smooth muscle cell growth kit, by American Type Culture Collection (3 mentions) Endothelial cell growth kit, by American Type Culture Collection (3 mentions) β actin, by Cell Signaling Technology (3 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (3 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Time cells, by American Type Culture Collection (2 mentions) Human anti scarf1, by R&D Systems (2 mentions) Maf2409, by R&D Systems (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Rpmi 1640 medium, by American Type Culture Collection (2 mentions) L glutamine, by American Type Culture Collection (2 mentions) Thp 1, by American Type Culture Collection (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (2 mentions)

40 protocols using vascular cell basal medium

1

Vascular Cells Senescence Model

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Human primary VSMC and EC were purchased from ATCC or from Lonza. VSMCs were cultured in Vascular Cell Basal Medium (ATCC, LGC, Manassass, USA) supplemented as defined by the manufacturer. ECs were cultured in Vascular Cell Basal Medium (ATCC, LGC, Manassass, USA) supplemented as defined by the manufacturer. All cells were kept in humidified atmosphere (37°C and 5% CO2 in the air). The cells were passaged every 3-4 days and were seeded 24 hours before treatment at a density of 3-3,5×103 cells/cm2 (VSMCs) and 10×103 cells/cm2 (ECs). Cells at early passages (5-9) was described as young cells, at passages 10-14 as middle passage, at late passages (15-19) as senescent cells and cells at passages 20 or more as extremely senescent. Cell morphology was analyzed in an inverted light microscope Nikon. Curcumin was dissolved in DMSO and the final concentration of DMSO in cell culture did not exceed 0.1%. Doxorubicin was dissolved in the medium.
Grabowska W., Suszek M., Wnuk M., Lewinska A., Wasiak E., Sikora E, & Bielak-Zmijewska A. (2016). Curcumin elevates sirtuin level but does not postpone in vitro senescence of human cells building the vasculature. Oncotarget, 7(15), 19201-19213.
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2

Cell Spiking of HUVEC and HPAEC

Cited 2 times
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HPAEC (cat #PCS-100-022) and HUVEC (cat#PCS-100-013) cells were purchased from ATCC and cultured in Vascular Cell Basal Medium (cat#PCS-100-030) supplemented with VEGF (Endothelial Cell Growth Kit-VEGF, cat#PCS-100-041). Normal blood donor (NBD) peripheral blood samples were collected in Streck cfDNA blood collection tubes at the Scripps NBD Service and processed as previously published [28 (link),29 (link)]. Cell-line cells were spiked into the NBD sample at 100 cell/mL (HUVEC) and 430 cells/mL (HPAECs).
Welter L., Zheng S., Setayesh S.M., Morikado M., Agrawal A., Nevarez R., Naghdloo A., Pore M., Higa N., Kolatkar A., Thiele J.A., Sharma P., Moore H.C., Richer J.K., Elias A., Pienta K.J., Zurita A.J., Gross M.E., Shishido S.N., Hicks J., Velasco C.R, & Kuhn P. (2023). Cell State and Cell Type: Deconvoluting Circulating Tumor Cell Populations in Liquid Biopsies by Multi-Omics. Cancers, 15(15), 3949.
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3

Vascular Smooth Muscle Cell Culture

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Human aortic VSMCs, vascular cell basal medium and smooth muscle growth supplement (SMGS) were purchased from ATCC (Manassas, VA). SMGS constituents and their final concentrations after addition to vascular cell basal medium were as follows: 5% FBS (vol/vol), 5 ng/ml human basic fibroblast growth factor, 5 ng/ml human epidermal growth factor, 5 μg/ml insulin, 50 μg/mL ascorbic acid, 10 mM L-Glutamine. VSMCs (passages 3-5) were incubated in vascular cell basal medium containing SMGS (complete medium) and 5.5 mM D-glucose along with antibiotic/antimycotic solution in a humidified atmosphere of 95% air and 5% CO2 at 37°C. After the attainment of confluence (~6-7 days), VSMCs were trypsinized, centrifuged, and seeded onto petri dishes or multiwell plates. Subconfluent VSMCs were maintained under SMGS (serum)-deprived conditions for 48 hours to achieve quiescence, and then subjected to treatments as described in the legends to the respective figures. DMSO (0.1%) was used as the vehicle control for PIO or ROSI treatment.
Osman I, & Segar L. (2015). Pioglitazone, a PPARγ agonist, attenuates PDGF-induced vascular smooth muscle cell proliferation through AMPK-dependent and AMPK-independent inhibition of mTOR/p70S6K and ERK signaling. Biochemical pharmacology, 101, 54-70.
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4

Culturing HUVECs in Endothelial Growth Medium

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HUVECs were obtained from the Department of Cardiology of The Second Hospital of Jilin University and cultured in a humidified incubator at 5% CO2 and 37°C. The complete growth medium for this cell line consisted of the vascular cell basal medium (ATCC, USA) with the endothelial cell growth kit-BBE (ATCC, USA) and contained the following components: 2% fetal bovine serum, 0.2% bovine brain extract, 10 mmol/L L-glutamine, 1 μg/mL hydrocortisone hemisuccinate, 5 ng/mL rhEGF, 50 μg/mL ascorbic acid, and 0.75 units/mL heparin sulfate.
Li T., Song X., Zhang J., Zhao L., Shi Y., Li Z., Liu J., Liu N., Yan Y., Xiao Y., Tian X., Sun W., Guan Y, & Liu B. (2017). Protection of Human Umbilical Vein Endothelial Cells against Oxidative Stress by MicroRNA-210. Oxidative Medicine and Cellular Longevity, 2017, 3565613.
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5

HUVEC Oxidative Stress Response

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HUVECs were purchased from the China Center for Type Culture Collection (Wuhan, China) and cultured in a humidified 5% CO2, 37 °C incubator. The vascular cell basal medium (ATCC, USA) added with the endothelial cell growth kit-BBE (ATCC, USA) was used as the complete growth medium for this cell line, and contained the following components: 0.2% bovine brain extract; 5 ng/mL rh EGF; 10 mmol/L l-glutamine; 0.75 units/mL heparin sulfate; 1 µg/mL hydrocortisone hemisuccinate; 2% fetal bovine serum, and 50 µg/mL ascorbic acid. HUVECs were cultured with different concentrations of H2O2 (0, 500, 1000, 2000, 3000, 4000, 5000, or 6000 µmol/L) for 24 h. Each experiment was performed in triplicate.
Li T., Diao H., Zhao L., Xing Y., Zhang J., Liu N., Yan Y., Tian X., Sun W, & Liu B. (2017). Identification of suitable reference genes for real-time quantitative PCR analysis of hydrogen peroxide-treated human umbilical vein endothelial cells. BMC Molecular Biology, 18, 10.
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6

Culturing Human Aortic Smooth Muscle Cells

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Human aortic smooth muscle cells (American Type Culture Collection, Cat. No. ATCC PCS-100-012, Manassas, VA) were cultured and proliferated in Vascular Cell Basal Medium (ATCC PCS-100-030) supplemented with the components of the Vascular Smooth Muscle Cell Growth Kit (ATCC PCS-100-042. Experiments were performed with SMCs at the fifth passage.
McCormick S., He Q., Stern J., Khodarev N., Weichselbaum R, & Skelly C.L. (2015). Evidence for the Use of Multiple Mechanisms by Herpes Simplex Virus-1 R7020 to Inhibit Intimal Hyperplasia. PLoS ONE, 10(7), e0130264.
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7

Culturing Human Umbilical Vein Endothelial Cells

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Pooled human umbilical vein endothelial cells (Lonza, C2519A or Sigma, 200P-05N) were cultured at 37 °C and 5% CO2 and per manufacture recommendations. EBM-2 media (Lonza, CC-3156) supplemented with EGM-2 SingleQuots (Lonza, CC-4176) or Vascular Cell Basal Medium (ATCC, PCS-100–030) supplemented with Endothelial Cell Growth Kit-VEGF (ATCC, PCS-100–041) were used. Cells between passages 3–6 were used for the experiments.
Nawara T.J., Sztul E, & Mattheyses A.L. (2024). Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells. bioRxiv.
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8

Detailed Cell Culture Protocol

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Reagents were purchased from Sigma-Aldrich unless otherwise stated. Complete media consisted of RPMI-1640 (Gibco, Thermo-Fisher, Grand Island, NY) supplemented with 100 U/mL penicillin, 100 U/mL streptomycin, 2 mM Glutamax and 10% fetal bovine serum (FBS, Hyclone GE Lifescience, Pittsburgh, PA). TIME cells (Cat#: CRL-4025) were obtained from the ATCC (Manassas, VA). Culture of the cell line required Vascular Cell Basal Medium (ATCC, Cat#: PCS-100-030), supplemented with microvascular endothelial cell growth kit-VEGF (ATCC, Cat#: PCS-110-041). Mouse embryonic fibroblasts (MEFs; Cat# SCRC-1008) were obtained from the ATCC. Flow cytometry antibodies were obtained from BD Biosciences. Human anti-SCARF1 (Cat#: AF2409 and MAF2409) antibodies were purchased from R&D Systems.
Jorge A.M., Lao T., Kim R., Licciardi S., ElKhoury J., Luster A., Means T.K, & Ramirez-Ortiz Z.G. (2022). SCARF1-induced efferocytosis plays an immunomodulatory role in humans, and autoantibodies targeting SCARF1 are produced in patients with systemic lupus erythematosus. Journal of immunology (Baltimore, Md. : 1950), 208(4), 955-967.
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9

Cell Culture Conditions for Human Cell Lines

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Human colon cancer cell lines (HCT116 and SNU-C1), dermal fibroblasts (HDFs), and keratinocytes (HaCaTs) were maintained in Dulbecco’s modified eagle medium-high glucose (Gibco, NY, USA) supplemented with 10% fetal bovine serum (Millipore, MA, USA), 100 units/mL penicillin, and 100 μg/mL streptomycin (Gibco, NY, USA). Human umbilical vein endothelial cells (HUVECs) were cultured in vascular cell basal medium (ATCC, VA, USA) supplemented with endothelial cell growth kit-VEGF (ATCC, VA, USA). Human monocytes (THP-1 cell line) were incubated with Roswell Park Memorial Institute-1640 (Gibco, NY, USA) supplemented with 10% fetal bovine serum (Millipore, MA, USA), 100 units/mL penicillin, and 100 μg/mL streptomycin (Gibco, NY, USA). All cells were maintained at 37°C in 5% CO2.
Kim M.S., Yang S.J., Jung S.Y., Lee T.Y., Park J.K., Park Y.G., Woo S.Y., Kim S.E, & Lee R.A. (2024). Combination of phytochemicals, including ginsenoside and curcumin, shows a synergistic effect on the recovery of radiation-induced toxicity. PLOS ONE, 19(1), e0293974.
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10

Vascular Cell Signaling Pathway Analysis

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Vascular cell basal medium (ATCC PCS-100-030), endothelial cell growth kit-BBE (ATCC PCS-100-040), Penicillin-Streptomycin-Amphotericin B Solution (ATCC PCS-999-002), and Phenol red (ATCC PCS-999-001) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Gibco (Gibco BRL, Life Technologies, Inc., NY, USA). D-glucose, Mannitol, polyvinylidenedifluoride (PVDF) membrane, and enhanced chemiluminescence (ECL) were purchased from Merck Millipore (Merck, Darmstadt, Germany). Primary antibodies against phosphorylated-PI3K, phosphorylated-Akt, total-Akt, phosphorylated-ERK1/2, total-ERK1/2, p53, caspase 3, caspase 9, Bax, Bcl 2, and β-Actin were purchased from Cell Signaling Technology (Cell Signaling Technology, Inc., Danvers, MA, USA). Other chemicals and reagents were purchased from Sigma Aldrich (Sigma, St. Louis, MO, USA).
Nensat C., Songjang W., Tohtong R., Suthiphongchai T., Phimsen S., Rattanasinganchan P., Metheenukul P., Kumphune S, & Jiraviriyakul A. (2021). Porcine placenta extract improves high-glucose-induced angiogenesis impairment. BMC Complementary Medicine and Therapies, 21, 66.
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