Animal experiments were approved by approved by The West China Hospital of Sichuan University Biomedical Research Ethics Committee (No. 2018039A) and all the procedures were conducted as previously described,31 (link) following the guidelines for animal care and use of laboratory animals. Six-week-old female Sprague–Dawley rats (260–280 g) were anesthetized with ketamine/xylazine (100 and 5 mg/kg body weight, respectively) by intraperitoneal injection. An access opening was made on the occlusal surface of the left mandible first molars. Then, 0.1 mL of log-phased E. faecalis V583 and ASwalR bacterial suspensions were inoculated into pulp chamber and covered with light-cure flowable resin. Four weeks post-operation, the rats were scarified and the imaging of the rats was taken using the Quantum GX Micro-CT System (PerkinElmer, Waltham, MA). Rats were sedated using isoflurane (1–5%) and oxygen (2 L/min) mixture during imaging procedures. The scanning conditions were used as following: kV = 90; CT μA = 72; 360° scan time = 8 s. We analyzed the reconstructed images with Analyze 12.0 (PerkinElmer, Waltham, MA). The values of relative periapical cavity were calculated when compared to the control groups.
Quantum gx micro ct system
Manufactured by PerkinElmer
Sourced in United States
The Quantum GX micro-CT system is a high-resolution, non-invasive 3D imaging solution designed for small animal research. The system utilizes X-ray technology to generate detailed three-dimensional images of small sample volumes, enabling researchers to visualize and analyze the internal structures and properties of their specimens.
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Lab products found in correlation
6 protocols using quantum gx micro ct system
1
Rat Model of Endodontic Infection
2
Quantifying Femur Bone Microarchitecture
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Femur samples were scanned with Quantum GX micro-CT system (PerkinElmer, Massachusetts, USA) at a voxel size of 10 µm. Quantum and Caliper Analyze software provided with
the instrument were used for scan and analysis. BMD, ratio of bone volume to total volume (BV/TV) and trabecular number (Tb.N) were calculated.
the instrument were used for scan and analysis. BMD, ratio of bone volume to total volume (BV/TV) and trabecular number (Tb.N) were calculated.
3
Quantitative 3D Microstructural Analysis
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3D images of the rat tibias were taken using the Quantum GX Micro-CT System (PerkinElmer, Waltham, MA) as previously described [20 (link)]. The rats were sedated using an isoflurane (1–5%) and oxygen (2 L/min) mixture during the imaging procedures and placed in the supine position inside the cabinet. The scanning conditions were used as follows: kV = 90, CT μA = 72, and 360° scan time = 8 s. We analyzed the three-dimensional reconstruction images with Analyze 12.0 (PerkinElmer, Waltham, MA). The bone density around the infective sites was calculated by the percent bone volume (BV) divided by total volume (TV, %).
4
Femur Microstructure Analysis by microCT and Histology
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Left femurs were fixed with 4% PFA for 24 h, and scanned by micro-CT using a Quantum GX microCT system (PerkinElmer, Waltham, MA, USA). The bone mineral density (BMD), bone volume/total volume (BV/TV), and trabecular number (Tb.N), trabecular thickness (Tb.th) and decreased trabecular separation/spacing (Tb.Sp) were measured.
Right femurs were demineralized in 10% ethylenediaminetetraacetic acid for 2 weeks. They were then embedded in paraffin, cut into 5 μm-thick sections, and stained with H&E and TRAP. Staining was visualized using a high-quality microscope (RVL-110-G; ECHO, San Diego, CA, USA).
Right femurs were demineralized in 10% ethylenediaminetetraacetic acid for 2 weeks. They were then embedded in paraffin, cut into 5 μm-thick sections, and stained with H&E and TRAP. Staining was visualized using a high-quality microscope (RVL-110-G; ECHO, San Diego, CA, USA).
5
Micro-CT Analysis of Transplanted Grafts
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All samples were scanned using a Quantum GX micro-CT system (PerkinElmer, USA). The samples were scanned under the following conditions: acquisition: 36; recon: 10; voxel size: 10.0 μm; scan mode: high resolution for imaging of the transplanted drafts or maxillary bone. Morphologic observation and measurements were assessed using Quantum GX software. Three-dimensional (3D) reconstruction was done with Mimics. We carefully defined the root length methods, which started from enamel-cementum junction and ended at apical foramen in sagittal section. All measurements were performed by one analyst.
6
Micro-CT and Histopathological Analysis of Rat Ankle Joints
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The hind limb of each rat was harvested after sacrifice and scanned using a PerkinElmer Quantum GX micro-CT system (Norwalk, CT, USA) with the following parameters: X-ray, 90 kV, and 80 μA; field of view (FOV), 72 mm; voxel size, 45-90; scan mode, high resolution; and scan time, 4 min. The 3D reconstruction images were analyzed using the PerkinElmer Analyze software (version 12.0, Norwalk, CT, USA). For histopathological analysis, ankle joints were fixed in 10% formalin after removing the skin and excess tissue. After decalcified in 10% ethylenediaminetetraacetic acid (EDTA) for one month, the samples were embedded in paraffin, sliced into 5 μm sections, stained with H&E, and examined under Nikon TS2 light microscope and high-resolution digital camera system (Nikon TS2, Tokyo, Japan).
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