Odyssey equipment

Manufactured by LI COR

Sourced in United States

The Odyssey equipment by LI-COR is a suite of products designed for high-performance fluorescence imaging and detection. The Odyssey systems utilize near-infrared fluorescence technology to enable sensitive and quantitative analysis of proteins, nucleic acids, and other biomolecules.

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Lab products found in correlation

Nitrocellulose membrane, by Thermo Fisher Scientific (1 mentions) Anti pgk1, by Abcam (1 mentions) Image studio lite ver 5, by LI COR (1 mentions) Anti cbp, by Merck Group (1 mentions) Anti gfp, by Merck Group (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Ab133303, by Abcam (1 mentions) Ab41787, by Abcam (1 mentions) Iblot2 protein transfer system, by Thermo Fisher Scientific (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Image studio software, by LI COR (1 mentions) Rabbit anti gapdh antibody, by Cell Signaling Technology (1 mentions) P1046, by Beyotime (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions)

Spelling variants of this product

Odyssey® CLx equipment (9 citations) Odyssey infrared imaging equipment (5 citations) Odyssey® CLx imaging equipment (3 citations) LI-COR Odyssey Fc equipment (2 citations)

5 protocols using odyssey equipment

Immunoblot Analysis of Yeast Complexes

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Yeast whole-cell lysates, the fractions from the glycerol gradient sedimentation, and the coimmunoprecipitated proteins were resuspended in SDS sample buffer, resolved on 8 or 10% SDS-PAGE (Tris-glycine running buffer), transferred to nitrocellulose membranes (Ambion) and incubated with anti-CBP (Millipore), anti-GFP (Sigma-Aldrich), anti-uL18 (gift from Dr Cleslei F. Zanelli, UNESP), anti-Nog1 (gift from Dr John L. Woolford Jr., Carnegie Mellon University) and anti-Pgk1 (Abcam). Secondary antibodies conjugated to IR700dye (anti-rabbit IgG, LI-COR) or IR800dye (anti-mouse IgG, LI-COR) were employed, and near-infrared Western blot detection was carried out using ChemiDoc MP Imaging System (BioRad) or Odyssey equipment (LI-COR). Images were processed using Image Studio™ Lite (ver. 5.2) software (LI-COR).

Bagatelli F.F., de Luna Vitorino F.N., da Cunha J.P, & Oliveira C.C. (2021). The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events. Nucleic Acids Research, 49(12), 7053-7074.

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Western Blotting of Cellular Proteins

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Adherent cells were briefly washed twice with PBS and lysed with 1% SDS lysis buffer containing 10 mM EDTA and 50 mM Tris-HCl, pH 8.0. Lysates were collected, briefly sonicated, then incubated at 95°C for 10 min and the protein concentrations were determined by BCA Protein Assay kit (Pierce). Calibrated samples were diluted with 4x LDS sample buffer (Novus), separated by SDS-PAGE using NuPAGE 4-12% Bis-Tris protein gels (Novus), and transferred to nitrocellulose or PVDF membranes by iBlot2 protein-transfer system (Thermo Fisher Scientific). Membranes were blocked with 50% Odyssey blocking buffer (LiCor) diluted with 0.1% Tween-20-containing TBS (TBST) and immunoblotted with antibodies against POR (Abcam, ab133303, rabbit monoclonal antibody, clone number UOTR1B493, Lot #GR290350-7, used at 1:1000 dilution), GPX4 (Abcam, ab41787, rabbit polyclonal antibody, Lot #GR56784-1, used at 1:1000 dilution), and β-Actin (8H10D10, #3700 and 13E5, #4970, Cell Signaling Technologies, used at 1:5000 dilution). Membranes were then washed with TBST and incubated with IRDye 800CW goat-anti-Rabbit or 680RD donkey-anti-Mouse secondary antibodies (LiCor). Immunoblotting images were acquired on an Odyssey equipment (LiCor) according to the manufacturer’s instructions, and analyzed in the ImageStudio software (LiCor). β-Actin was used as loading controls.

Zou Y., Li H., Graham E.T., Deik A.A., Eaton J.K., Wang W., Sandoval-Gomez G., Clish C.B., Doench J.G, & Schreiber S.L. (2020). Cytochrome P450 oxidoreductase contributes to phospholipid peroxidation in ferroptosis. Nature chemical biology, 16(3), 302-309.

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Western Blot Analysis of IL-21 Receptor

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The cells were harvested and centrifuged at 700 x g for 10 min at 4°C. The supernatant was removed, and the pellet was treated with denaturing buffer (160 mM Tris-HCl pH 6.8, 4% SDS, 10% b-mercaptoethanol, 24% glycerol 24%, and 0.02% bromophenol blue). Lysates were separated by 13% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto a nitrocellulose membrane. The rabbit anti-IL21R antibody (1 : 200; Proteintech), rabbit anti-GAPDH antibody (1 : 1000; Cell signaling), and IRDye® 800CW Goat anti-Rabbit IgG Secondary Antibody (1 : 10000) were used as secondary antibodies. The infrared signal was captured with LI-COR Odyssey equipment. ImageJ software (https://imagej.nih.gov/ij/) was used for the quantitative analyses, and the gel images were quantified based on the linear signal ranges.

Falavinha B.C., Barisón M.J., Rebelatto C.L., Marcon B.H., de Melo Aguiar A., da Silva E.B., Stimamiglio M.A, & Shigunov P. (2022). Interleukin 21 Receptor Affects Adipogenesis of Human Adipose-Derived Stem/Stromal Cells. Stem Cells International, 2022, 4930932.

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Renal Angiotensin II Receptor Activation

Cited 1 time

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To determine whether renal angiotensin II receptors are activated by long term dietary sodium intake, conformation state-sensitive anti-AT₁R and anti-AT₂R antibodies^{24 (link),25 (link)} (Proteimax Biotechnology, Cotia, São Paulo, Brazil) were used. These antibodies are sensitive to activity-mediated conformational changes in the receptors being able to specifically recognize the activated state of the receptor, as previously documented^{26 (link)–29 (link)}. Deparaffinized and rehydrated sections were incubated with blocking solution (1% bovine serum albumin + 5% sucrose in PBS) and then with conformation-specific antibodies against the angiotensin II receptors 1 and 2 (AT₁R and AT₂R), conjugated with DY-682 (red) and DY-800 (green) fluorophores, respectively. Sections were then washed with PBS, and fluorescence intensity measured using Odyssey equipment (LI-COR Biosciences) as described by us in previous studies^{24 (link),25 (link)}. The intensity of each sample was normalized to negative control (i.e. sections treated only with secondary antibody labelled with fluorescent Alexa Fluor dyes DY-682 and DY-800).

Katayama I.A., Huang Y., Garza A., Brooks D.L., Williams J.S., Nascimento M.M., Heimann J.C, & Pojoga L.H. (2023). Longitudinal Changes in Blood Pressure are Preceded by Changes in Albuminuria and Accelerated by Increasing Dietary Sodium Intake. Experimental gerontology, 173, 112114.

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Protein Extraction and Western Blot Analysis

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RIPA lysis buffer (P0013B, Beyotime, Jiangsu, China) mixed with phosphatase and protease inhibitors (P1046, Beyotime, Jiangsu, China) was used to lyse the cells on ice. Afterwards, sodium dodecyl sulfate (SDS) was added to the total protein after it had been measured. Then, 40 µg of protein was transferred to nitrocellulose membranes (HATF85R, Millipore, Bedford, MA, USA) after being separated on SDS polyacrylamide gel electrophoresis. The membranes were then blocked and treated with primary antibodies at 4 °C for an overnight period. The membranes were then cleaned and treated with secondary antibodies that had been colored fluorescently (Licor, USA). Finally, an Odyssey equipment (Licor, USA) was used to see the bands on the membranes. The Appendix 1 contains a collection of information about primary antibodies.

Xie Y., Cui Z., Fang S., Zhu G., Zhen N., Zhu J., Mao S., Sun F., Pan Q, & Ma J. (2023). Anti-ferroptotic PRKAA2 serves as a potential diagnostic and prognostic marker for hepatoblastoma. Journal of Gastrointestinal Oncology, 14(4), 1788-1805.

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