Monoclonal mouse anti gapdh

Western immunoblot analysis was performed using whole-cell lysates derived from HGSOC cell lines as previously described with minor alterations.^{15 (link)} To lyse cells grown as spheroids, 1% sodium dodecyl sulfate was added to the lysis buffer. Approximately 40 μg of protein lysate per cell line was analyzed. Equal loading was verified by incubating the membranes with anti-GAPDH antibody. Primary antibodies used include monoclonal mouse anti-GAPDH (GeneTex, Irvine, CA, USA; cat. no. GTX627408), monoclonal rabbit anti-E-cadherin (Cell Signaling, Danvers, MA, USA; cat. no. 3195), polyclonal rabbit anti-SUSD2 (Prestige Antibodies Sigma-Aldrich Corp.; cat. no. HPA004117) and polyclonal rabbit anti-N-cadherin (Cell Signaling; cat. no. 4061). Secondary antibodies used were Pierce (Thermo-Fisher Scientific, Waltham, MA, USA; cat. no. 31329) for GAPDH. Pierce cat. no. 31345 was used for N-cadherin, E-cadherin and SUSD2. Experiments were performed using three biological replicates for each cell line.

Western immunoblot analysis was performed as previously described [24 (link)]. Equal loading was verified using anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody. Primary antibodies include: monoclonal mouse anti-MCP-1 (R&D Systems, Minneapolis, MN) and monoclonal mouse anti-GAPDH (GeneTex, Irvine, CA).

Western immunoblot analysis was performed using whole cell lysates derived from HGSOC cell lines as previously described with minor alterations [6 (link)]. Forty μg of protein lysate/cell line was analyzed. Equal loading was verified by incubating the membranes with anti-GAPDH antibody. Primary antibodies used include monoclonal mouse anti-GAPDH (GeneTex, Irvine, CA, USA), Cat #GTX627408, polyclonal rabbit anti-SUSD2 (C-term. fragment; Prestige Antibodies Sigma-Aldrich Corp.), monoclonal mouse anti-SUSD2 (C-term. fragment; Bio-Techne Corp.) Cat MAB9056, polyclonal rabbit anti-SUSD2 (N-term. fragment; AbCam technologies, San Francisco, CA, USA) Cat ab182147.
For immunoblotting performed in the characterization of SUSD2 expression in stable HGSOC cells, detection was achieved with IRDye-conjugated secondary antibodies (LI-COR Biosciences) and imaged using the infrared Odyssey system; 800CW donkey anti-mouse antibody, and a 680RD donkey anti-rabbit antibody. Experiments were performed using three biological replicates for each cell line.