Ezradp 62k

Manufactured by Merck Group

Sourced in United States

The EZRADP-62K is a laboratory equipment product from Merck Group. It is a multi-channel pipette that can accurately and precisely dispense liquids across a range of volumes. The core function of the EZRADP-62K is to facilitate liquid handling tasks in various laboratory settings.

Automatically generated - may contain errors

Lab products found in correlation

Ezrl 83k, by Merck Group (3 mentions) Ezrmi 13k, by Merck Group (3 mentions) Non esterified free fatty acids nefa colorimetric kit, by PSC Biotech (2 mentions) Sypro ruby blot stain, by Thermo Fisher Scientific (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions)

5 protocols using ezradp 62k

Quantitative Biomarker Measurements in Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quantitative estimations of leptin (cat. EZRL-83K; Millipore, St. Charles, MO, USA), adiponectin (cat. EZRADP-62K, Millipore, MO, USA), C-reactive protein (cat. CYT294; Millipore, St. Charles, MO, USA) and PAI-1 serum levels (cat. Ab198509, Abcam, Cambrige, UK) were performed following the manufacture’s protocols using specific ELISA kits.

Grasa-López A., Miliar-García Á., Quevedo-Corona L., Paniagua-Castro N., Escalona-Cardoso G., Reyes-Maldonado E, & Jaramillo-Flores M.E. (2016). Undaria pinnatifida and Fucoxanthin Ameliorate Lipogenesis and Markers of Both Inflammation and Cardiovascular Dysfunction in an Animal Model of Diet-Induced Obesity. Marine Drugs, 14(8), 148.

+ Open protocol

+ Expand

Plasma Biomarker Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma adiponectin, insulin, and leptin concentrations were determined using specific enzyme-linked immunosorbent assay kits (EZRADP-62K, EZRMI-13K, and EZRL-83K, respectively) from Millipore (Billerica, MA, USA). Insulin growth factor 1 (IGF1) determination was carried out using enzymatic kit (ab213902, Abcam, Cambridge, UK). Non-esterified fatty acid (NEFA) colorimetric kit was obtained from BiooScientific (Austin, TX, USA) and Uric acid kit from SpinReact (Girona, Spain) (Ref. no. 1001010). Plasma samples were assayed in duplicate.

Roglans N., Baena M., Sangüesa G., Velázquez A.M., Griñán-Ferré C., Pallàs M., Sánchez R.M., Alegret M, & Laguna J.C. (2021). Chronic liquid fructose supplementation does not cause liver tumorigenesis but elicits clear sex differences in the metabolic response in Sprague–Dawley rats. Food & Nutrition Research, 65, 10.29219/fnr.v65.7670.

+ Open protocol

+ Expand

Western Blot Analysis of Adiponectin Receptors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein lysates were subjected to separation using sodium dodecyl sulfate-polyacrylamidegel electrophoresis, transferred to polyvinylidene fluoride membranes and blocked in 5% non-fat dry milk for 2 hours at room temperature as described, previously ^{30 (link), 31 (link)}. Membranes were probed overnight at 4°C with primary antibodies at a dilution of 1:1000 for AdipoR1 (42 kDa, AdipoR1-2; Alpha Diagnostics), 1:2000 for AdipoR2 (42 kDa, AdipoR2-1; Alpha Diagnostics), 1:500 for phosphorylated AMPK (phospho-AMPK, Thr-172; 62kDa; 2535; Cell Signaling), and 1:1000 for AMPK (63 kDa, 07-350; Millipore). Membranes were then incubated with HRP-linked anti-rabbit secondary antibody at a dilution of 1:20,000 for 1 h at 28°C and visualized using ECL (GE Healthcare). Densitometry was performed using Scion Image (NIH). To correct for potential protein loading errors all membranes were stained with Sypro Ruby blot stain (Invitrogen) and densitometry was performed as previously described ^{30 (link), 31 (link)}.Rat serum was assessed for total APN concentration utilizing a kit (EZRADP-62K; Millipore) per manufacturer instructions.

Tomicek N.J., Hunter J.C., Machikas A.M., Lopez V, & Korzick D.H. (2014). Acute Adiponectin Delivery is Cardioprotective in the Aged Female Rat Heart. Geriatrics & gerontology international, 15(5), 636-646.

+ Open protocol

+ Expand

Metabolic Biomarkers in Blood Assessment

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum adiponectin, insulin, and leptin concentrations were determined using specific ELISA kits (EZRADP-62K; EZRMI-13K; and mEZRL-83K, respectively) from Millipore (Billerica, MA, USA). The non-esterified free fatty acids (NEFA) colorimetric kit was from Bioo Scientific (Austin, TX, USA), and the kits for alanine (ALT) and aspartate aminotransferase (AST) kinetics were from SpinReact (Girona, Spain). The insulin sensitivity index (ISI) was calculated as 2/[insulin (nM) × blood glucose (µM) + 1].

Velázquez A.M., Bentanachs R., Sala-Vila A., Lázaro I., Rodríguez-Morató J., Sánchez R.M., Laguna J.C., Roglans N, & Alegret M. (2022). KHK, PNPLA3 and PPAR as Novel Targets for the Anti-Steatotic Action of Bempedoic Acid. Biomedicines, 10(7), 1517.

+ Open protocol

+ Expand

Adiponectin, Insulin, and Leptin Biomarkers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum adiponectin, insulin, and leptin concentrations were determined using specific ELISA kits (EZRADP‐62K; EZRMI‐13K; EZRL‐83K, respectively) from Millipore (Billerica, MA, USA). The non‐esterified free fatty acids (NEFA) colorimetric kit was from BiooScientific (Austin, TX, USA) and the kits for alanine (ALT) and aspartate aminotransferase (AST) kinetics were from SpinReact (Girona, Spain). The insulin sensitivity index (ISI) was calculated as 2/ [insulin (nM) X blood glucose (µM) + 1].^[⁴⁴^]

Velázquez A.M., Bentanachs R., Sala‐Vila A., Lázaro I., Rodríguez‐Morató J., Sánchez R.M., Alegret M., Roglans N, & Laguna J.C. (2022). ChREBP‐driven DNL and PNPLA3 Expression Induced by Liquid Fructose are Essential in the Production of Fatty Liver and Hypertriglyceridemia in a High‐Fat Diet‐Fed Rat Model. Molecular Nutrition & Food Research, 66(7), 2101115.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!