Anti srebp 2 sc 13552

The tissue was harvested from the kidney cortex of the sacrificed animals. Protein was extracted for western blotting from the cortex. The primary antibodies used in this study were Fatty Acid and Lipid Metabolism Antibody Sampler Kit (#8335), anti-TNFR1 (#13377), anti-AMPK (#5832), and anti-p-AMPK (#2535), from Cell Signaling Technology (Danvers, MA, USA); anti-SREBP-1 (sc-13551) and anti-SREBP-2 (sc-13552), from Santa Cruz Biotechnology (Dallas, TX, USA); anti-CD36 (NB400-144ss), anti-β actin (NB600-501), and anti-α tubulin (NB100-690), from Novus Biologicals (Littleton, CO, USA); anti-PPARα (GTX01098), anti-HDAC1 (GTX100513), and anti-histone H3 (GTX122148), from GeneTex (Irvine, CA, USA); and anti-KIM 1 (ab190696, Abcam, Cambridge, UK). The membrane was then subjected to the secondary antibody, either anti-mouse IgG or anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, USA), which was dissolved in 5% skim milk in TBST for 1 h. Next, the membrane was incubated for 1–2 min in enhanced chemiluminescence mixture (JT96-K004M, T-Pro Biotechnology, Zhonghe, New Taipei City, Taiwan) for visualization. The western blotting assay was repeated at least three times.

A standard Western blotting protocol was used as described previously [41 (link),42 (link)]. Subcellular fractionation experiments to estimate expression of these molecules in the nuclear fraction including Lipin-1, SREBP-1/2, PPARα using The primary antibodies used in this study included the Fatty Acid and Lipid Metabolism Antibody Sampler Kit (#8335), anti-AMPK (#5832), and anti-p-AMPK (#2535) from Cell Signaling Technology (Danvers, MA, USA); anti-SREBP-1 (sc-13551) and anti-SREBP-2 (sc-13552) (Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-CD36 (NB400-144ss), Anti-Lipin 1 antibody (ab70138), anti-β actin (NB600-501), and anti-α tubulin (NB100-690, Novus Biologicals, Littleton, CO, USA); anti-PPARα (GTX01098), anti-HDAC1 (GTX100513), anti-lamin B1 antibody (ab65986), and anti-histone H3 (GTX122148) (GeneTex, Irvine, CA, USA); and anti-KIM 1 (ab190696, Abcam, Cambridge, MA, USA). The secondary antibody used was either anti-mouse IgG or anti-rabbit IgG (Jackson ImmunoResearch, West Grove, PA, USA), which was dissolved in 5% skim milk in TBST for 1 h, followed by incubation for 1–2 min in enhanced chemiluminescence mixture (JT96-K004M, T-Pro Biotechnology, Zhonghe, New Taipei City, Taiwan) for visualization. The Western blot was repeated at least three times.

Anti SREBP1 (ab3259), and LAMP1 (ab24170) antibodies were purchased from Abcam, Cambridge, UK. Anti SREBP2 (sc13552) and β-actin (sc47778) were purchased from Santa Cruz Biotechnology. Anti ACC1 (4190), phospho-ACC1 Ser79 (3661), AMPKα1 (5832), phospho-AMPKα Thr172 (2535), LC3B (2775), Nibrin (14956) and α-Tubulin (2125) were purchased from Cell Signaling Technology. Anti SQSTM1/p62 Ab (P0067), metformin (PHR1084), dorsomorphin (P5499), Lovastatin (PHR1285), T0901317 (T2320) and Bafilomycin A1 (B1793) were purchased from Merck Life Science. Risperidone (S1615), olanzapine (S2493), ziprasidone (S1444) and U18666A (S9669) were purchased from DivBioScience.