Male Zucker lean and Zucker diabetic fatty rats were purchased from Charles River Laboratories (Wilmington, MA). Rats were housed in a temperature-controlled room with a 12:12-h light-dark cycle and free access to Purina 5008 rat chow and water. Urine was collected over a 24-h period in metabolic cages and stored at -80°C until use. Blood glucose was monitored using the Accu-chek glucometer by tail-vein blood sampling. This study was carried out in strict accordance with the recommendations in the Guide of the Care and Use of Laboratory Animals of the National Institutes of Health. All animal protocols were approved by the Institutional Animal Care and Use Committee of the Morehouse School of Medicine (approval number 12–27). All surgery was performed under sodium pentobarbital anesthesia, and all efforts were made to minimize suffering.
Zucker diabetic fatty rats
Manufactured by Charles River Laboratories
Sourced in United States, Morocco
The Zucker Diabetic Fatty (ZDF) rat is a laboratory animal model used in research. It is a genetic model of type 2 diabetes mellitus. The ZDF rat exhibits hyperglycemia, hyperinsulinemia, and dyslipidemia, making it a valuable tool for studying the development and progression of diabetes and associated metabolic disorders.
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6 protocols using zucker diabetic fatty rats
1
Urine Collection and Blood Glucose Monitoring in Zucker Rats
2
Zucker Diabetic Fatty Rat Model Study
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The Harvard Medical Area Standing Committee on Animals prospectively approved all studies. Nine-week-old Sprague Dawley (Harlan Laboratories, Frederick, MD) and obese (fa/fa) Zucker Diabetic Fatty rats (Charles River Laboratories, Raleigh, NC) were acclimatized to a constant temperature and humidity (22°C and 70% respectively) for 5 days, with a fixed 12:12 light: dark cycle (lights on at 7am). All animals were male. The initial average body weights for SD and ZDF rats were 285.6±2.4g and 314.3±4.6g, respectively. A formula diet (Purina 5008: Carbohydrate 56.5%, Protein 26.8%, Fat 16.7%) was provided ad libitum except for the five days following surgery (as described below). Animals were caged in pairs pre-operatively and individually after surgery.
3
Metabolic Disorders in Obese and Diabetic Rats
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Obese male Zucker fatty (fa/fa—8 weeks of age) and Zucker Diabetic Fatty rats (ZDF—12 weeks of age), and Koletsky spontaneous hypertensive rats (f/f—16 weeks of age) and their age-matched lean controls were purchased from Charles River Laboratories. Rats were fed ad libitum a regular chow diet and kept in a 12-h dark–light cycle. All procedures were approved by the Institutional Animal Care and Utilization Committee at the University of Toledo College of Medicine and Life Sciences (formerly known as the Medical College of Ohio). All experiments were conducted in accordance with the recommendations of the committee, confirming to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85-23, revised 1996).
4
Zucker Diabetic Fatty Rat Model Experiments
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All the experimental procedures on animals were performed according to protocols approved by the Animal Care and Use Committee of the Benemerita Universidad Autonoma de Puebla, identification code: BERRSAL71, 18-05-2017. Every effort was made to minimize the number of animals used and to ensure minimal pain and/or discomfort. Experiments were carried out in male Zucker Diabetic Fatty (ZDF) rats (2–3 months old) from Charles River Laboratories, California, USA Throughout the text, diabetic-obese ZDF rats (ZDF-Leprfa/fa) will be designated as OZDF rats, and lean controls, non-obese non-diabetic ZDF (ZDF-Lepr+/+) as LZDF. The rats were kept at the University Animal Core Facilities under controlled environmental temperature and, exposed to light-dark cycles of 12 h, with ad libitum consumption of water and Purina 5008 chow.
5
Zucker Diabetic Fatty Rat Model for T2DM
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For T2DM 7-week-old Zucker Diabetic Fatty (ZDF) rats (Charles River) were used [6 (link)]. Animals develop T2DM due to a genetic mutation of the leptin receptor. Homozygous recessive males (fa/fa) develop obesity, fasting hyperglycemia and T2DM. Homozygous dominant (+/+) and heterozygous (fa/+) lean genotypes remain normoglycemic. Animals were fed a special diet (Purina #5008) and water ad libitum. Animals were assigned to the following groups: non-diabetic control lean animals (T2DM Co; n = 8) and animals with type-2 DM (T2DM; n = 7). Experimental procedures were performed at the age of 32 weeks (after 25 weeks of diabetes duration).
6
Zucker Diabetic Fatty Rat Model
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5 week old male Zucker Diabetic Fatty (ZDF) rats weighing about 200-220 g were purchased from Charles River (Wilmington, MA) and allowed 2 days for environmental and trainer handling acclimation. The rats were maintained under standard housing conditions at 22 ± 2°C with 12/12-h light/dark cycles and fed a high-calorie Purina 5008 lab chow diet (Charles River). At 14 weeks of age the ZDF rats became diabetic (blood glucose >300 mg/dL) (T2D) after which they were fasted overnight and euthanized by exposure to isoflurane (Webster Veterinary Supply Inc., Devens, MA).
In both T1D and T2D animals blood glucose levels were assessed from blood obtained via tail incision using an Advantage Accu-Chek glucometer (Boehringer Mannheim Corp., Indianapolis, IN). After the experiment blood was collected via heart puncture with a 19 1/2 -gauge needle into EDTA Vacutainer tubes. Plasma was isolated after centrifuging the blood in a 4°C centrifuge at 1500 rpm for 10 min. An aliquot of blood from rats in each group was sent to the clinical laboratory of our LSUHSC-Shreveport facility for clinical tests to determine blood glucose levels, liver function, and kidney function.
In both T1D and T2D animals blood glucose levels were assessed from blood obtained via tail incision using an Advantage Accu-Chek glucometer (Boehringer Mannheim Corp., Indianapolis, IN). After the experiment blood was collected via heart puncture with a 19 1/2 -gauge needle into EDTA Vacutainer tubes. Plasma was isolated after centrifuging the blood in a 4°C centrifuge at 1500 rpm for 10 min. An aliquot of blood from rats in each group was sent to the clinical laboratory of our LSUHSC-Shreveport facility for clinical tests to determine blood glucose levels, liver function, and kidney function.
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