Log In Sign up
The largest database of trusted experimental protocols

Mts assay

Manufactured by Merck Group
Visit Supplier
Sourced in United States

The MTS assay is a colorimetric method for determining the number of viable cells in proliferation or cytotoxicity experiments. It utilizes the tetrazolium compound MTS, which is reduced by living cells into a colored formazan product that can be measured spectrophotometrically.

Automatically generated - may contain errors

Lab products found in correlation

Mts assay, by Promega (2 mentions) Emetine, by Merck Group (1 mentions) Dihydrotestosterone dht, by Merck Group (1 mentions) Rpmi medium, by Merck Group (1 mentions) Enzalutamide, by Selleck Chemicals (1 mentions) Concanamycin a, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Merck Group (1 mentions) Expire technology, by PerkinElmer (1 mentions) Prism software, by GraphPad (1 mentions) Brdu assay, by Abcam (1 mentions) Elx808iu microplate reader, by Agilent Technologies (1 mentions) Mtt assay, by Merck Group (1 mentions) Pure nitrogen, by Airgas (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Victor x3 2030, by PerkinElmer (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Basic fibroblast growth factor (bfgf), by Merck Group (1 mentions) Ebm 2, by Lonza (1 mentions) Mscgm, by Lonza (1 mentions)

Close spelling variants of this product

MTS Assay Kit (3 citations)

20 protocols using mts assay

1

Cell Viability Assay with Emetine

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (5 × 103) were seeded in 96-well plates and allowed to adhere overnight at 37 °C. These cells were treated with distilled water or emetine (Sigma-Aldrich) at the indicated concentrations for 48 hours. Cell viability was measured by an MTS assay (Sigma-Aldrich). All experiments were performed in triplicate.
Wu T.H., Chang S.Y., Shih Y.L., Huang T.W., Chang H, & Lin Y.W. (2019). Emetine Synergizes with Cisplatin to Enhance Anti-Cancer Efficacy against Lung Cancer Cells. International Journal of Molecular Sciences, 20(23), 5914.
+ Open protocol
+ Expand
2

MTS Assay for Cell Growth Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
A (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay (Sigma-Aldrich, St Louis, MO, USA) was used to measure the growth rates of cells. The cells which were collected after transfection with indicated siRNAs and recombinant lentiviruses were plated into 96-well plates in triplicate at 1 × 103 per well. After 24 h, 20 μL of MTS (5 mg/ml) was added to cells for quantifying cell proliferation from 1 to 7 days. The cells were incubated with MTS for 3 h in 5% CO2 at 37°C. Finally, optical absorbance of each well was measured at 490 nm using a microplate reader. Cell growth curves were made by plotting the absorbance (ordinate) against time (abscissa). Three independent experiments were performed to analyze the cell growth.
Zhang H.X., Jiang S.S., Zhang X.F., Zhou Z.Q., Pan Q.Z., Chen C.L., Zhao J.J., Tang Y., Xia J.C, & Weng D.S. (2015). Protein kinase CK2α catalytic subunit is overexpressed and serves as an unfavorable prognostic marker in primary hepatocellular carcinoma. Oncotarget, 6(33), 34800-34817.
+ Open protocol
+ Expand
3

Cell Proliferation Assay with MTS

Check if the same lab product or an alternative is used in the 5 most similar protocols
The (3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium) (MTS) assay (Sigma-Aldrich, St Louis, MO, USA) was used to measure the growth rates of cells. The cells that were collected after transfection with the indicated siRNAs were plated onto 96-well plates in triplicate at 1 × 103 cells per well. After 24 h, 20 μL of MTS (5 mg/ml) was added to the cells to quantify cell proliferation from 1 to 6 days. The cells were incubated with MTS for 3 h in 5% CO2 at 37°C. Finally, the optical absorbance of each well was measured at 490 nm using a microplate reader. Cell growth curves were made by plotting the absorbance (ordinate) against time (abscissa). One independent experiment in triplicate was performed to analyze cell growth.
Ma Z., Wang X., He J., Xia J, & Li Y. (2017). Increased expression of protein kinase CK2α correlates with poor patient prognosis in epithelial ovarian cancer. PLoS ONE, 12(3), e0174037.
+ Open protocol
+ Expand
4

MTS Assay for Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell survival was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Sigma). For the MTS assay (Promega), 1 × 104 cells/well were seeded in triplicate in a 96-well plate for each cell line. At 12, 24, and 48 h, 20 μL of MTS reagent was added to each well and incubated for 1 h at 37 °C; the results were analyzed by a plate reader at 490 nm. The sample data were normalized to the background readings of media only.
Yao T., Lu R., Li Y., Peng Y., Ding M., Xie X, & Lin Z. (2015). ALDH1 might influence the metastatic capability of HeLa cells. Tumour Biology, 36(9), 7045-7051.
+ Open protocol
+ Expand
5

Cytotoxicity Assessment of Antibody-Drug Conjugates

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells lines were plated at 1–3 × 103 cells per well in complete growth medium containing 10% FCS in 96-well plates and allowed to adhere overnight. Cells were treated with cetuximab, cisplatin or ADCs in a 1:3 dilution to a maximum dose of 100 μg/mL. Proliferation was assessed with the MTS assay (Sigma-Aldrich, St. Louis, MO, USA), as previously described [45 (link)].
Chia P.L., Parakh S., Tsao M.S., Pham N.A., Gan H.K., Cao D., Burvenich I.J., Rigopoulos A., Reilly E.B., John T, & Scott A.M. (2020). Targeting and Efficacy of Novel mAb806-Antibody-Drug Conjugates in Malignant Mesothelioma. Pharmaceuticals, 13(10), 289.
+ Open protocol
+ Expand
6

MTS Assay for Cell Viability

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cell survival was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)- 2(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Sigma). For the MTS assay (Promega), 1 × 104 cells per well from each cell line were seeded triplicately in a 96-well plate. At 1st d, 2nd d,3rd d, 4th d and 5th day, 20 μL of MTS was added to each well and incubated for 1 h at 37 °C; the results were analyzed by a plate reader at 490 nm. The sample data were normalized to the background readings of media only [16 (link)].
Yao T., Weng X., Yao Y., Huang C., Li J., Peng Y., Lin R, & Lin Z. (2020). ALDH-1-positive cells exhibited a radioresistant phenotype that was enhanced with hypoxia in cervical cancer. BMC Cancer, 20, 891.
+ Open protocol
+ Expand
7

Cell Viability Assay with YC-1

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded at a density of 1 × 104 cells per well in a 96-well plate and cultured overnight. These cells were treated with DMSO or YC-1 (Sigma-Aldrich) at the indicated concentrations for 24 and 48 h. The cell viability was measured by an MTS assay (Sigma-Aldrich). All experiments were performed in triplicate.
Wu J.Y., Shih Y.L., Lin S.P., Hsieh T.Y, & Lin Y.W. (2019). YC-1 Antagonizes Wnt/β-Catenin Signaling Through the EBP1 p42 Isoform in Hepatocellular Carcinoma. Cancers, 11(5), 661.
+ Open protocol
+ Expand
8

Evaluating Cell Viability by MTS Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell viability was analyzed by MTS assay (Sigma). Cells (1500–3000) were plated in each well of a 96-well tissue culture plate with 100 μL of medium. The next day, the medium was replaced with 100 μL of fresh medium containing 10 μM Olaparib or 0.5 μM gamma secretase inhibitor, as indicated, and the cells were grown for 7 days. Stock Olaparib and gamma secretase inhibitor were prepared in DMSO. At the end of the treatment period, 10 μL of MTS solution was added to each well, the cells were incubated at 37°C for 1 to 2 h, and absorbance was read at 490 nm. Data are presented as a percentage of the control cells cultivated under the same conditions or the absorbance of the wells.
Decker J.T., Hobson E.C., Zhang Y., Shin S., Thomas A.L., Jeruss J.S., Arnold K.B, & Shea L.D. (2017). Systems Analysis of Dynamic Transcription Factor Activity Identifies Targets for Treatment in Olaparib Resistant Cancer Cells. Biotechnology and bioengineering, 114(9), 2085-2095.
+ Open protocol
+ Expand
9

Cell Viability Assay for HEK-293 and Prostate Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK-293 and LNCaP/DuCaP/22Rv1 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma) and Roswell Park Memorial Institute (RPMI) medium (Sigma) respectively, supplemented with 10% fetal bovine serum (FBS) with 4 mM or 2 mM L-glutamine respectively and 1 mM pyruvate. Where indicated, media was changed at 24 hours to phenol-red free RPMI-1640 (Life Technologies) supplemented with 10% charcoal stripped FBS (CSS-RPMI) (Life Technologies). LNCaP F877L/T878A and LNCaP control empty plasmid cells were a gift from Novartis (5 (link)). All cell lines were regularly mycoplasma tested and passaged between 15-30 times for all experiments. Enzalutamide was purchased from Selleckchem, bafilomycin-A1 (baf-A1) from Melford and dihydro-testosterone (DHT) and concanamycin-A from Sigma Aldrich. Compounds were dissolved in dimethyl sulfoxide (DMSO). Cell viability was assessed using the MTS assay (Sigma) as per the manufacturer’s instructions.
Whitton B., Okamoto H., Rose-Zerilli M., Packham G, & Crabb S.J. (2021). V-ATPase inhibition decreases mutant androgen receptor activity in castrate-resistant prostate cancer. Molecular cancer therapeutics, 20(4), 739-748.
+ Open protocol
+ Expand
10

Cell Proliferation Assay of HR-8348 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
The proliferation of HR-8348 cells, LV10N-hsa-miR-96-5p-inhibitor HR-8348 (HR-8348-IN) cells, and LV10N-hsa-miR-96-5p-NC HR-8348 (HR-8348-NC) cells were examined by MTS assay (Sigma-Aldrich, USA) at the indicated time points, accordingly to the descriptions of the MTS assays of Cory (27 (link)) and McCauley et al. (28 (link)). Cells were seeded at a density of 1.0×103 cells/well in 96-well culture plates, and cultured for 0/24/48/72/96h. The absorbance values were determined on a microtiter plate reader (Expire Technology, Perkin Elmer) at 492 nm. Three independent experiments were done in triplicate.
Wu F., Wu B., Zhang X., Yang C., Zhou C., Ren S., Wang J., Yang Y, & Wang G. (2021). Screening of MicroRNA Related to Irradiation Response and the Regulation Mechanism of miRNA-96-5p in Rectal Cancer Cells. Frontiers in Oncology, 11, 699475.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!